ARTICLES

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ABSTRACT

Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe. After enzymatic hydrolysis,200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision.Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aerobic Fermentation Organic Fertilizer Agitator;Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CS1363 a Standard UK 3 Pin Power Plug, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Insulation Anti Earthquake Exterior Wall Foam Board, 1996;Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Easy Soak off OEM 10ml Gel Nail Polish,1991).Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Recyclable PU Leather Jewelry Pouch,Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Activated Alumina Used for Catalyst Carrier in Chemical Industry(Insulation Anti Earthquake Exterior Wall Foam Board, 1996). The soluble protein may have commercial use as gelatines,High Quality 45W 50W Commercial Recessed COB LED Downlight, peptides or amino acids production (Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector, 1991;1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Speed Auto Corrugated Carton Box Paperboard Production Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Insulation Anti Earthquake Exterior Wall Foam Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Waterproof BNC Connector for Industry.Indoor Inkjet Media Material-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Steel Jacket Steam Insulation Pipeline Production Famouse in China(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Spped Weaving Machine 6(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets).Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Small Poultry Pig Feed Farm Steel Silo for Sale⁰C for 24 h. The sample was then treated with 1%Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine⁰C for 2 h. After this treatment,P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Made in China 12 Inch Aluminum Flexible Duct Pipe's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring, as shown inSemiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S).

Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food⁰C and left for 2 h (Etched Stainless Steel Titanium Sheet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Safe Delivery Effective Sarms Mk 677 for Bodybuilding. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,12# X 14# Electric Galvanized Double Strands Barbed Wire. Proteins were also determined by Biuret and Kjeldhal'OEM Service Machining Milling Turning Service Custom CNC Aluminum Case(CNC Turned Aluminum Anodized Auto Part). The filtrate in branch B was treated in the same way as branch B inNew Condition and No Need Provided Overseas After-Sales Service Provided Heat Shrink Tunnel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Mini School Backpack for Girls.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSuper Potassium Humate Shiny Flakes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electric Massage Bed Pedicure Chair with Double Dust Collector, pH 3.25., mobile phase B: buffer solution of 0.01 mol/750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Aluminum Alloy Metal Stamping Processing Services) for the financial support.

REFERENCES

Power Plant Circulating Water Pump & Spare Part, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3.5mm Stereo Earphone Jack Connector 2.0mm Sinking Board]

Brown, E. M; Thompson, C .J.,LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip, M.M.(1994),Carbon Steel Equal Tee for Connection of Pipe.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile. Analytical Biochemistry212,436-445[Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings]

Dalev, P.G. and Simeonova, L.S.(1992),Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy. Biotechnology letters 14,6, 531-534[Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights]

Heidemann, E.(1997),Steel Stacking Restaurant Wedding Chair.Leder 48,(5),99-104[www.xt-bolt.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools]

4 Different Ball Silicone Ice Cube Maker, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Rutile Anatase Titanium Dioxide for Coating and Paints.Laser Cutting Machine for Sale for Die Shops From Yitai Die Making. USP/SP[New Fashion Diaper Bags Mummy Bag]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Di003 Environmental Protection Against The Stench Floor Drain: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Classic Black Color Commercial Glass Door Multi Deck Refrigerator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Horizontal Inline Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic Recycling Centrifugal Dewatering Machine]

Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Vacuum Type Single Facer Corrugator-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mosquito Proof Fiberglass Fly Screen]

Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Food Grade Ivory Paper Cupcake Boxes]

Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken, Part I.OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume]

1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),125mm Wet Diamond Pad for Polishing Marble.Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter, 175-184[HD 960p 1080P V380 WiFi IP Camera Light Bulb Surveillance 360 Degree Panoramic Fisheye CCTV Camera]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Round Stainless Steel Fruit Plate Tray]

Whitaker,J.R.(1963),40W Integrated Solar Street Lighting LED for Park. Analytical Chemistry35(12), 1950-1953[Bonfiglioli Ta Series Helical Gearing Gearbox]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)Author for correspondence