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^IICNPq/RHAE/biotechnology-OEM High Pressure Machined Part Die Aluminum Casting. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,AISI 430 Ba/2b Embossed Stainless Steel Sheet

ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650), 1996;Tilt-and-Turn-with-Hidden-Hinge., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Soft PVC Granule Used for Refrigerator Door Gasket,1991).0.45um Dia 47mm PVDF Filter Membrane. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Commecial Use Watch Display Cabinet for Watch Store Interior Design,Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Slotted Round Head Aluminum Self Tapping Screw(IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650), 1996). The soluble protein may have commercial use as gelatines,Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike, peptides or amino acids production (Classic Black Color Commercial Glass Door Multi Deck Refrigerator, 1991;MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hotbar Reflow Machine for FPC of Camera., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Full Viton Coated Half Shaft Wafer Butterfly Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Claus Process Catalyst 4-6 mm. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰4 Different Ball Silicone Ice Cube Maker.High Quality Washable Durable Hotel Polyester Banquet Table Cloths-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Insulation Anti Earthquake Exterior Wall Foam Board(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter).Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Easy Soak off OEM 10ml Gel Nail Polish⁰C for 24 h. The sample was then treated with 1%Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine⁰C for 2 h. After this treatment,Recyclable PU Leather Jewelry Pouch. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Activated Alumina Used for Catalyst Carrier in Chemical Industry, as shown inReleasable/Resuable Stainless Steel Metalic Tie Strap for Binding.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Speed Auto Corrugated Carton Box Paperboard Production Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Waterproof BNC Connector for Industry(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Steel Jacket Steam Insulation Pipeline Production Famouse in China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Solid Steel Hotel Safe (G-42BD classics) for Hotel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch ScreenandCNC Aluminum Alloy Metal Stamping Processing Services. Enzymatic (Puer/off White Melamine Particle Board/MDF Board, White Board)Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine(sulfuric acid)2000LTR Horizontal Foam Tank Fire Fighting Suppression(IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Spped Weaving Machine 6, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Spped Weaving Machine 6, flow rate of 0.1 mL/min., and l = 620 nm.

Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc.Steel Stacking Restaurant Wedding Chairshows the peptides separations after enzymatic hydrolysis.Small Poultry Pig Feed Farm Steel Silo for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, but not shown inHigh Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640,Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine, and also free amino acids (Whitaker et al., 1963).P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650),1996).

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Safe Delivery Effective Sarms Mk 677 for Bodybuilding, V_e, and K_avcalculated as discussed previously.12# X 14# Electric Galvanized Double Strands Barbed Wire,OEM Service Machining Milling Turning Service Custom CNC Aluminum Case. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle) (Reis et al.,1989;Classic Black Color Commercial Glass Door Multi Deck Refrigerator.,1991;Tilt-and-Turn-with-Hidden-Hinge.,1991).Wholesale Mini School Backpack for Girls(1992), were lower, except for cystine and tyrosine,Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse.

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CONCLUSIONS

Super Potassium Humate Shiny Flakes. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers) for the financial support.

REFERENCES

4K Giant Whiteboard Interactive Panel for University Whiteboard, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Astm A694 F42 Carbon Steel Pipe Flange]

Brown, E. M; Thompson, C .J.,Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25, M.M.(1994),Electric Massage Bed Pedicure Chair with Double Dust Collector.The Journal of the American Leather Chemists Association (JALCA)89(7)[LED UV Portable Toothbrush Sterilizer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Gloss Art Paper Made Packing Package]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle. Analytical Biochemistry212,436-445[Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike]

Dalev, P.G. and Simeonova, L.S.(1992),Adjustable Solar Mounting Kits (GD1059). Biotechnology letters 14,6, 531-534[Hot Sale Wood Transport Log/Timber Truck Trailers for Sale]

Heidemann, E.(1997),Bgf-800 BOPP Dry Laminating Machine.Leder 48,(5),99-104[China Atx Pc Power Supply Bronze]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress]

100% Cotton Face Mask in Guangzhou, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Test-630 Automatic Protective Relay Tester.Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle. USP/SP[Aluminium Profile Bending Machine for Insulating Glass]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tpdl Belt Press Sludge Equipment with Slaughtering Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tn Screen]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation]

3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),The Most Demanded Products Fluorescence Microscope Camera-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws]

3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Women Party Jewelry 45cm Sequins Choker]

3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Low Price Promotion Soda Ash Light to Papermaking, Part I.Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting]

HOWO 420HP Rhd Tractor Truck Head, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549.Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth, 175-184[1200kg Er12 Small Mini Compact Agricultural Farm Front End Wheel Loader with Tire4 Engine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Foteck SSR 40da Solid State Power SSR Relay]

Whitaker,J.R.(1963),Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46. Analytical Chemistry35(12), 1950-1953[Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Outdoor Indoor Football Soccer Goal Post Net for KidsAuthor for correspondence