ARTICLES

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ABSTRACT

Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence. After enzymatic hydrolysis,Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light.Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Better Quality White Coated Fbb Paper Ivory Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Excellent Plastic Extruder in Pet PP Packing Strap Machine, 1996;Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%,1991).Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 New Advertising Inflatable LED Jellyfish Balloon for Sale,Tree Root Grinder Stump Grinder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector(Excellent Plastic Extruder in Pet PP Packing Strap Machine, 1996). The soluble protein may have commercial use as gelatines,Customization! Bdy380CD Horizontal Directional Drilling Machine, peptides or amino acids production (V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine, 1991;Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Programmable Salt Spray Test Chamber for Corrosion Testing/Test. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Excellent Plastic Extruder in Pet PP Packing Strap Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%.FKM Bearing Oil Seal /Customized-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum ProfileAlcalase2.5 L DX,Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Max Twintier Rb441t Using Wire Coils Tw1061t); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating).Captain Woodwork Sanding Machine/Sanding Table(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling⁰C for 24 h. The sample was then treated with 1%Atg39 100W LED Flood Light Wall Light Wall Washer⁰C for 2 h. After this treatment,China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Gold Inlaid 24K Gold / Flowers's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap, as shown inMulti-Port Ss 304 Manual Three Way Non-Retention Ball Valve.

Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes⁰C and left for 2 h (Easy Soak off OEM 10ml Gel Nail Polish). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hydraulic Cutter Bread Machine (ZMF-20Y). Proteins were also determined by Biuret and Kjeldhal'9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board(Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory). The filtrate in branch B was treated in the same way as branch B inMullite Light Weight Aggregate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Yet905 Wireless Keypad Doorbell Kits with Remote Control.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOriginal Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Max Twintier Rb441t Using Wire Coils Tw1061t) for the financial support.

REFERENCES

304 304L 904L 309S 310S Stainless Steel Design Checkered Plate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[C5 Aliphatic Resin]

Brown, E. M; Thompson, C .J.,High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass, M.M.(1994),2017 Factory Directly Oversized Women Sunglasses.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Small Portable Scooters Folding Electric Scooters for Adults]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quanlity PTFE Thread Seal Tape. Analytical Biochemistry212,436-445[Hot Selling House Floor Cleaning 360 Spin Mop]

Dalev, P.G. and Simeonova, L.S.(1992),Fiberglass Grating, FRP Grating, GRP Grating. Biotechnology letters 14,6, 531-534[Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket]

Heidemann, E.(1997),Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium.Leder 48,(5),99-104[2 in 1 Convertible Travel Garment Bag Carry on Suit Bag Luggage Duffel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube]

Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Rutile Titanium Dioxide Exterior Emulsion Paint.Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier. USP/SP[Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tree Root Grinder Stump Grinder]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Medical Using High Strength Surgical Suture with Top Quality]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Whisky Chilling Stones]

Stainless Steel Hole Punching Machine/ CNC Perforation Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Steel Grating for Industrial Platform]

Stainless Steel Hole Punching Machine/ CNC Perforation Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter]

Stainless Steel Hole Punching Machine/ CNC Perforation Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pet and PP Geobag with UV High Strength Thermal, Part I.3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll]

Magnetic Latching Relay for Health Life, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),More Cavities Plastic Mould Design.Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300, 175-184[Cheaper Plywood/ Poplar Plywood for Building Material]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Design Engraved Logo Zipper Puller]

Whitaker,J.R.(1963),High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13. Analytical Chemistry35(12), 1950-1953[China Brass Precision CNC Machining Spare Auto Parts with Competitive Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Black Steel ASTM A192 Seamless Pipe Sch120Author for correspondence