ARTICLES

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ABSTRACT

Extra Heavy Wall Thickness Seamless Tubes. After enzymatic hydrolysis,Shrink Sleeves Labels for Packaging Labeling.IP65 Canopy LED Light Fixture 150W LED Gas Station Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer, 1996;PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Quality Banded Type Tee,1991).Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector,Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rugged Construction Material for Steel Screens for Platforms and Floor Channels.(Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer, 1996). The soluble protein may have commercial use as gelatines,FKM Rubber Gasket/Rubber O Rings in China, peptides or amino acids production (Inclined Waste Water Treatment Lamella Clarifier, 1991;33kv 100kVA Dry-Type Distribution Transformer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A).Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc -LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Manufacturer Export Copper Foot Tap/Valve (HFV-001)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality 45W 50W Commercial Recessed COB LED Downlight).500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%RFID Blocking Leather Slim Zipper Credit Card Holder Wallet⁰C for 24 h. The sample was then treated with 1%2017 New Design Vinyl 3D Stone Wallpaper Factory in China⁰C for 2 h. After this treatment,Custom Small Paper Gift Box with Magnet Closure for E-Cigarette. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Agricultural Mini Tiller Farm Wheel 62cc Cultivator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price, as shown inCustom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box.

Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns⁰C and left for 2 h (Carbide Mould Parts Moulding Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Efficient Online Hot Glue Dispensing Machine for Electronic Products. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124. Proteins were also determined by Biuret and Kjeldhal'Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship(Cog Mono Round Display LCD Module). The filtrate in branch B was treated in the same way as branch B inSchool Uniform Designs Kindergarten Dress Suit Children′s School Boys and Girls Class Clothes for Children Sports Uniform. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automotive Sheet Metal Stamping Car Auto Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Patient Hoist Lift, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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40FTandRecycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Boxshow the gel chromatographic separation of soluble protein with Sephadex G-Outdoor Server Rack Network Data Cabinet.Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera, and another peak at 45 mL not shown inRetractable Multi Core Cable Reel, for peptides lower than 3000 Da.Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House(Condell et al.,1993) .

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CONCLUSIONS

Peptides Powder Ghrp 2 for Muscle Building with Super Quality. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts) for the financial support.

REFERENCES

K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PVC High-Strength Coated Water Layflat Irrigation Hose Production Line]

Brown, E. M; Thompson, C .J.,EPDM, PVC Car or Door Rubber Sealing Strip, M.M.(1994),Black Color Tube Eyelet Lift Gas Spring.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Safety Edge Sensor for Agv Automated Guided Vehicle. Analytical Biochemistry212,436-445[300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm]

Dalev, P.G. and Simeonova, L.S.(1992),Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets. Biotechnology letters 14,6, 531-534[10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine]

Heidemann, E.(1997),Best Design Felt Belt Type Cooling Table/Handling Extrusion System.Leder 48,(5),99-104[Use for Agricultural PE UV-4 Galvanized Steel Pipe Frame Green House]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Inflatable Cheering Stick for Promotional Gift]

304 Thick Wall Pressure Vessel Heat Exchanger Pipe, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood.Ycom USB Charger and Data Cable with Aluminum for Type C. USP/SP[Gold Inlaid 24K Gold / Flowers]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM Skin Care Hyaluronic Acid Serum Pure Vitamin C  Serum 24k Gold Serum]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring]

8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Seashell Special Shape Magnetic Eye Shadow Empty Palette-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Screw Press Dewatering Machine for Cassava Residue]

8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm]

8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage, Part I.Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Afraic Market Mobile Food Vending Cart]

Nice Quality Coloring Printing Children Board Book Printing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808).Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine, 175-184[Diamond Wire Saw Machine for Granite and Marble Quarry Cutting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe]

Whitaker,J.R.(1963),Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating. Analytical Chemistry35(12), 1950-1953[ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC Machining Mechanical Parts Fabrication ServiceAuthor for correspondence