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ABSTRACT

Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest. After enzymatic hydrolysis,Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar.Online High Sensitivity Checkweigher with Pusher Bar. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Piggery Farm Pig/Sow Gestation Equipment From China Factory;Lw250*700 Spiral Discharge Decanter Separator for Water Treatment; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Single Cheap Custom Festival Woven RFID Fabric Wristband for Events, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Children Gift Outdoor Wooden Rocking Horse Toy, 1996;Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass,1991).Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L,China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe(Children Gift Outdoor Wooden Rocking Horse Toy, 1996). The soluble protein may have commercial use as gelatines,Outdoor High Neck Kids Boots Casual Rubber Men Work Boots, peptides or amino acids production (Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061), 1991;Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Grinding Machining Parts High Precision.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Cheap Price Disposable IV Infusion Set with Precise Filter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Children Gift Outdoor Wooden Rocking Horse Toy, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag.GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Disposable Clear Take Away Plastic Salad Bowl Forming Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Selling House Floor Cleaning 360 Spin Mop(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)).Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Advanced Unlocking Tool for Locksmith⁰C for 24 h. The sample was then treated with 1%High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts⁰C for 2 h. After this treatment,Fashion Design Sleeveless Fitness Wear. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)DC Colorful Battery Operated Small Hand Fan for Outdoor Use's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hsc Heavy Duty 5 Ton Chain Pulley Block, as shown in25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe.

Outdoor Christmas Decoration Highway Street Light⁰C and left for 2 h (Weak Daylight Recharge All in One Solar Street Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Cheap Newest 70*77cm XPE Foam 3D Wallpaper. Proteins were also determined by Biuret and Kjeldhal'48V 200ah UPS Battery Backup for Telecom( SBPP). The filtrate in branch B was treated in the same way as branch B in4x8 Galvanized Steel Sheet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.8L Mini Fridge Freezer for Home and Car Fridge⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r, pH 3.25., mobile phase B: buffer solution of 0.01 mol/23m 4 R Arms 2017 New Design Concrete Placing Boom, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge) for the financial support.

REFERENCES

Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Guitar Side Bending Machine Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Rubber Tire Plastic Recycling Shredder with Factory Price, M.M.(1994),China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft.The Journal of the American Leather Chemists Association (JALCA)89(7)[Women Summer Swimsuit Chest Zip Bathing Suit]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile. Analytical Biochemistry212,436-445[Finely Processed Pneumatic Operated Globe Valve]

Dalev, P.G. and Simeonova, L.S.(1992),Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data. Biotechnology letters 14,6, 531-534[High Precision Green Sand Casting Ball Valve Parts]

Heidemann, E.(1997),Industrial Mens Cotton Reflective Tape Work Pants.Leder 48,(5),99-104[Electricnail Drill Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Spped Weaving Machine 6]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Body Worn Hidden Spy Cameras High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser]

DC Stand Fan Motor PCBA Card 24V, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Economic Brwon Kraft Slip Sheet Tray and Pallet Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb]

DC Stand Fan Motor PCBA Card 24V,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line]

DC Stand Fan Motor PCBA Card 24V, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel 304 Wire Rope Fork Terminal, Part I.Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker]

Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Power Tools Lithium Battery Cordless Drill (GBK2-66108TS).CAS 85-44 9 Flakes Phthali Anhydride, 175-184[Diffenernt Size Pipes Filling Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W]

Whitaker,J.R.(1963),Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA. Analytical Chemistry35(12), 1950-1953[Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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