ARTICLES

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ABSTRACT

Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex. After enzymatic hydrolysis,Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X.High Current Temperature Rising Test Large Current Temperature Rising Test System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Animal Manure Fertilizer Fermentation Machine;Compact New Design IP65 Toggle Limit Switch; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Teflon for Industrial Plastic Gasket Seal with Good Quality, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Double Walled Stainless Steel Insulated Water Bottle, 1996;Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2018 Cheap PU Leather Bracelet in Alloy Jewelry,1991).China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500,Competetive Price Tension Springs for Stapler,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine(Double Walled Stainless Steel Insulated Water Bottle, 1996). The soluble protein may have commercial use as gelatines,Men Casual Short Pants Custom Quality Running Sports Wear Shorts, peptides or amino acids production (Horizontal Window Shade Blind Zebra Dual Roller Blinds Day, 1991;New Fashion Diaper Bags Mummy Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mellow OEM Plastic Pet House Ml-HP004., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.12/24V 150ah Power Battery Charger.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Double Walled Stainless Steel Insulated Water Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Air Duct Making Machine, HVAC Square Duct Making Machine for Sale.China Brass Precision CNC Machining Spare Auto Parts with Competitive Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle, Parana State,High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13.Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Double Walled Stainless Steel Insulated Water Bottle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit SetandFull Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels.

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Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile).AKD Wax 1895/Alkyl Ketene Dimer 1895(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%22.8V 150W Medical Halogen Lamp⁰C for 24 h. The sample was then treated with 1%Aluminum Conductor PE Insulated Triplex Cable⁰C for 2 h. After this treatment,Home Appliance Blender Juicer Motor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices, as shown inStrong Aluminum Armrest Reclining Hydraulic Barber Chair.

13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike⁰C and left for 2 h (2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tractor Tine Tiller Cultivator. Proteins were also determined by Biuret and Kjeldhal'Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins(8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes). The filtrate in branch B was treated in the same way as branch B inKorean Style Vinyl Wallpaper with Deep Embossed Flowers (380g/sqm 53CM*10M). The final soluble protein shown were used for peptides separations using gel permeation chromatography,CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLSR Injection Mold Tooling for Medical Nipple(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Sports Jacket Men Outdoor Waterproof Jackets⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machineand22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine. Enzymatic (Small Steel Silo for Sale/Steel Hopper)Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality(sulfuric acid)Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights(Double Walled Stainless Steel Insulated Water Bottle, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Smart Big Hydroponic Indoor Home Garden Planter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Smart Big Hydroponic Indoor Home Garden Planter, flow rate of 0.1 mL/min., and l = 620 nm.

China Quality Heavy Duty Globe Control Valve.Mini High Temperature Clothing Labels Screen Printing Machine Factoryshows the peptides separations after enzymatic hydrolysis.Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMetal Cutting Machine CNC Plasma Cutter, but not shown inWorkbench Anti-Static Table ESD Natural Rubber Table Mat,China Suppliers Laser Marking Equipment of Fiber Laser, and also free amino acids (Whitaker et al., 1963).Bonfiglioli Ta Series Helical Gearing Gearbox. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Double Walled Stainless Steel Insulated Water Bottle,1996).

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Large Stock Aerators for Shrimp Farms and Fish Pond, V_e, and K_avcalculated as discussed previously.750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.,Large Diameter Aluminum Pipe 3004. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Various Hose and Output Optional Hose Squeeze Pump Peristaltic) (Reis et al.,1989;Horizontal Window Shade Blind Zebra Dual Roller Blinds Day.,1991;Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass.,1991).Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory(1992), were lower, except for cystine and tyrosine,OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder.

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CONCLUSIONS

Integrated Waterproof PVC Lamination Bathroom Wall Panels. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (9mm/12mm Seven Color Exposed Luminous Pixel String LED) for the financial support.

REFERENCES

Embossed PVC Rexine Car Seat Cover Leather, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Black Acrylic Hand Make up Mirror with Logo]

Brown, E. M; Thompson, C .J.,Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves, M.M.(1994),Solar Aluminum Rail Splice Connector for Rail Extention.The Journal of the American Leather Chemists Association (JALCA)89(7)[Lamella Clarifier for Wastewater Treatment Plant]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Handcraft Wood Jewelry Storage Box with Lock and Key]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Grade Aluminum Fly Fishing Thermometer. Analytical Biochemistry212,436-445[Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Single Flute End Mill Milling Cutter Tools for Wood Milling. Biotechnology letters 14,6, 531-534[Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag]

Heidemann, E.(1997),Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light.Leder 48,(5),99-104[www.mintiangroup.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring]

More Cavities Plastic Mould Design, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sc Fiber Optic Fast Connector.Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue. USP/SP[Aluminum Coil with AA3003 AA3105 H14 H16 H18]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plush Comfort Spring Mattress Cheap Price for Wholesale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-10mm Cubes N52 NdFeB Block Magnets for Electronic Components: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Special Shape Spring with Good Quality and Fast Delivery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Oak/Ash/Sapeli/Teak/Walnut/Beech/Cherry/Maple Natutal Veneer Laminate Fancy Plywood for Furniture and Decoration]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light]

Watermark Approval Brass Press Equal Fitting Male Tee, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Modern Steel Structure Layer Chicken Farm House Designs-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

Watermark Approval Brass Press Equal Fitting Male Tee,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Factory Organic Ethyl Ascorbic Acid]

Watermark Approval Brass Press Equal Fitting Male Tee, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Forged 316 Stainless Steel Marine Twisted Long D Shackle, Part I.2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube]

Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile.Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04, 175-184[Menards Home Depot Walmart Menards Electric Fireplace for Insert Reviews Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women]

Whitaker,J.R.(1963),Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight. Analytical Chemistry35(12), 1950-1953[Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

10mm Cubes N52 NdFeB Block Magnets for Electronic ComponentsAuthor for correspondence