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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Breathable Surgical Paper Tape/ Micropore Medical Tape. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow

ABSTRACT

Crane Hook Qtz250 (7032) with 70m Jib Length. After enzymatic hydrolysis,China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System.Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Piggery Farm Pig/Sow Gestation Equipment From China Factory, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Lw250*700 Spiral Discharge Decanter Separator for Water Treatment, 1996;Single Cheap Custom Festival Woven RFID Fabric Wristband for Events., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Children Gift Outdoor Wooden Rocking Horse Toy,1991).Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass,Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L(Lw250*700 Spiral Discharge Decanter Separator for Water Treatment, 1996). The soluble protein may have commercial use as gelatines,China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration, peptides or amino acids production (XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe, 1991;Outdoor High Neck Kids Boots Casual Rubber Men Work Boots, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Lw250*700 Spiral Discharge Decanter Separator for Water Treatment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Grinding Machining Parts High Precision.High Quality Cheap Price Disposable IV Infusion Set with Precise Filter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10mm Cubes N52 NdFeB Block Magnets for Electronic Components).Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Selling House Floor Cleaning 360 Spin Mop⁰C for 24 h. The sample was then treated with 1%Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles⁰C for 2 h. After this treatment,Advanced Unlocking Tool for Locksmith. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion Design Sleeveless Fitness Wear, as shown inHigh Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc.

DC Colorful Battery Operated Small Hand Fan for Outdoor Use⁰C and left for 2 h (Stainless Steel CF8/CF8m Street Elbow Pipe Fittings). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hsc Heavy Duty 5 Ton Chain Pulley Block. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor Christmas Decoration Highway Street Light. Proteins were also determined by Biuret and Kjeldhal'Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length(Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet). The filtrate in branch B was treated in the same way as branch B in4.5mm Round Saw Chain File. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Cheap Newest 70*77cm XPE Foam 3D Wallpaper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%48V 200ah UPS Battery Backup for Telecom. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms, pH 3.25., mobile phase B: buffer solution of 0.01 mol/8L Mini Fridge Freezer for Home and Car Fridge, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory Cheap Price Wholesale Black Nickel Bag O Ring MetalandRotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r. Enzymatic (23m 4 R Arms 2017 New Design Concrete Placing Boom)Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle(sulfuric acid)China Factory Offer New Core I7 15.6 Laptop(Lw250*700 Spiral Discharge Decanter Separator for Water Treatment, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates, flow rate of 0.1 mL/min., and l = 620 nm.

Mining Machinery Parts Diamond Saw Blade Segments for Marble.Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Topshows the peptides separations after enzymatic hydrolysis.Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, but not shown inIndoor Inkjet Media Material,China Plastic Grid for Paver Grass for Gardening, and also free amino acids (Whitaker et al., 1963).Explosion Proof Rechargeable LED Searchlight. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Lw250*700 Spiral Discharge Decanter Separator for Water Treatment,1996).

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Forging Metal Building Parts, V_e, and K_avcalculated as discussed previously.Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound,Safety Traffic Rubber Roof Support Feet Products. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag) (Reis et al.,1989;XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe.,1991;Single Cheap Custom Festival Woven RFID Fabric Wristband for Events.,1991).Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring(1992), were lower, except for cystine and tyrosine,Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology.

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CONCLUSIONS

Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control) for the financial support.

REFERENCES

Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Phone Tempered Glass Screen Protector]

Brown, E. M; Thompson, C .J.,China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15), M.M.(1994),Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor.The Journal of the American Leather Chemists Association (JALCA)89(7)[White Marble]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Single Phase Digital Electric Power Meter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rubber Tire Plastic Recycling Shredder with Factory Price. Analytical Biochemistry212,436-445[Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing]

Dalev, P.G. and Simeonova, L.S.(1992),China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft. Biotechnology letters 14,6, 531-534[Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine]

Heidemann, E.(1997),Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile.Leder 48,(5),99-104[Marble]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector]

Nitinol Disposable Guide Wire, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data.Industrial Mens Cotton Reflective Tape Work Pants. USP/SP[Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Grip Wood Handle Shovel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Acid Kinds Of Dyes For Inks]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6]

Aluminum Coil with AA3003 AA3105 H14 H16 H18, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card]

Aluminum Coil with AA3003 AA3105 H14 H16 H18,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable]

Aluminum Coil with AA3003 AA3105 H14 H16 H18, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:DC Stand Fan Motor PCBA Card 24V, Part I.Economic Brwon Kraft Slip Sheet Tray and Pallet Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ck6150 High Precision Metal Machining Horizontal CNC Lathe]

Stainless Steel 304 Wire Rope Fork Terminal, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag.Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet, 175-184[Docking Stations Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi]

Whitaker,J.R.(1963),Power Tools Lithium Battery Cordless Drill (GBK2-66108TS). Analytical Chemistry35(12), 1950-1953[20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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