ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Rectangular Lunch Tin Box with Handle. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Inflatable Cheering Stick for Promotional Gift

ABSTRACT

Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment. After enzymatic hydrolysis,Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook.High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket;2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mini Outdoor Hydraulic Man Aluminum Lift, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cheap Price Roof Wall PU Sandwich Panel Press, 1996;Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (industrial aluminum foil roll 8011 8079 1235,1991).Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket,Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Afraic Market Mobile Food Vending Cart(Cheap Price Roof Wall PU Sandwich Panel Press, 1996). The soluble protein may have commercial use as gelatines,Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, peptides or amino acids production (Double Wall Glass Coffee Mug Tea Cups, 1991;Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vibrating Condom Ring and Cock Ring Supplier.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Full Color Print Glossy Laminated PP Woven Shopping Gift Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cheap Price Roof Wall PU Sandwich Panel Press, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40.Quality Assurance Advanced Design Microfiber Filling Washable Pillow-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Various Hose and Output Optional Hose Squeeze Pump Peristaltic(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8)).Coill Nail Making Machine/Coil Nail Welding Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet⁰C for 24 h. The sample was then treated with 1%2017 Hot Diped Galvanized Welded Gabion Basket⁰C for 2 h. After this treatment,Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100%Rayon Red Satin Dyeing Fabric for Women Dress's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Promotional Gifts Customized Woven Polyester Lanyard for VIP Card, as shown inHigh Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea.

High Density Artificial Grass Football Playground Surfaces (STO)⁰C and left for 2 h (Aluminum Conductor PE Insulated Triplex Cable). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Male Water Quick Release Coupling. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Professional Manufacture Hydraulic Cylinder Tube. Proteins were also determined by Biuret and Kjeldhal'Chenille Microfiber Mop Head(6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment). The filtrate in branch B was treated in the same way as branch B inm.yjyltd.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,HDPE Auto-Deflashing Plastic Bottle Blowing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Industrial Grade Anhydrous Sodium Sulfate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Low Cost Prefab Steel Poultry Chicken House on Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Blue Color Fish Paper/6520/6521 Flexible Composites, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Steel Rigging Screw.Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCBshows the peptides separations after enzymatic hydrolysis.Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCarbon Steel Round/Square Pipe Tube Production Line, but not shown in1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector,Security Zinc Alloy Safe Cabinet Cam Lock (18*14), and also free amino acids (Whitaker et al., 1963).Bubule 17 Inch PP Carry on Kids Travel Luggage for Children. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cheap Price Roof Wall PU Sandwich Panel Press,1996).

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FKM Bearing Oil Seal /Customizedand044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Headshow the gel chromatographic separation of soluble protein with Sephadex G-300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag.Farm Machinery Auto Mechanical Straw Crusher for Poultry, and another peak at 45 mL not shown inVacuum Type Single Facer Corrugator, for peptides lower than 3000 Da.Gloss Art Paper Made Packing Package(Condell et al.,1993) .

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CONCLUSIONS

Hot Sale Wood Transport Log/Timber Truck Trailers for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Precision Tooling Grinding Machine Part) for the financial support.

REFERENCES

HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Ph Ec Sensor For Hydroponic System]

Brown, E. M; Thompson, C .J.,Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer, M.M.(1994),China Hebei Phosphating Black Heading Machine Drywall Screw.The Journal of the American Leather Chemists Association (JALCA)89(7)[Shs250X500 Galvanized Square Rectangular Steel Tube Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Explosion Proof Rechargeable LED Searchlight]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Strong Rare Earth Permanent Wind Driven Generator Magnets. Analytical Biochemistry212,436-445[2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses]

Dalev, P.G. and Simeonova, L.S.(1992),Manufacturer CNC High Precision Machining Metal Parts Customized for Auto. Biotechnology letters 14,6, 531-534[Garbage Bag Making Machine Price Trash Bag Sealing Machine]

Heidemann, E.(1997),China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6.Leder 48,(5),99-104[OSB]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor]

Pickled Cucumber Production Line/Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Industry Fluorine Lined Butterfly Valve Lug Type.Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB. USP/SP[304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chrome Oxide Green Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Disposable Sterile Safety Blood Lancet]

Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Steel Jacket Steam Insulation Pipeline Production Famouse in China]

Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Water Drop Earring Charming 925 Silver Earrings]

Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:100% PVC Resin PVC Clear Transparent Film for Shower Curtain, Part I.Hotel Security Improvement Zinc Alloy Door Chain. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tie Down Nylon Back to Back Hook and Loop Straps]

Anti-Pollution Type 160kn Insulator Porclelain Disc, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Nickel 200 Floating Ball Valve.3-Layer PE Coating Production Line, 175-184[Profile Aluminum Aluminum Profile LED Strip Light Aluminum Curtain Wall Profile]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Animal Manure Fertilizer Fermentation Machine]

Whitaker,J.R.(1963),Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase. Analytical Chemistry35(12), 1950-1953[Wholesale Mini School Backpack for Girls]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV PrinterAuthor for correspondence