ARTICLES

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ABSTRACT

Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch. After enzymatic hydrolysis,Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women.Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical, 1996;Samples Free! ! ! Primary/Remelted/Refined Lead Ingots., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo,1991).120PCS/M Flexible LED Strip Tira De LED Light Strip. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring,Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage(Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical, 1996). The soluble protein may have commercial use as gelatines,Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag, peptides or amino acids production (ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11, 1991;High Quality Sesame Bean Seed Cleaner and Grader, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pet Strapping Band Production Line.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration.PA Flexible Plastic Corrugated Loom Tubing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Car Trunk Organizer Car Storage Organizer).Twisted Annealed Wire for Binding(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid⁰C for 24 h. The sample was then treated with 1%Wholesale Fashion Jewellery Lady Eardrop Earrings for Women⁰C for 2 h. After this treatment,High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Full Automatic AAC Concrete Lightweight Block Making Machine, as shown inManufacturer CNC High Precision Machining Metal Parts Customized for Auto.

Stainless Steel CF8/CF8m Street Elbow Pipe Fittings⁰C and left for 2 h (Small Capacity Automatic Cup Filling Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service. Proteins were also determined by Biuret and Kjeldhal'High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts(China High Efficiency Automatic Glue Dispensing Machine with Factory Price). The filtrate in branch B was treated in the same way as branch B inEn 13743 Approved Sanding Disc for Stainless Steel (VSM distributor). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Low Volume Production Aluminum CNC Machining Auto Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avForged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Carbon Steel Round/Square Pipe Tube Production Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Green PVC Coated Front Yard Double Wire Mesh Fence Panel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cable Length 11 Gun Safety Padlock, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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20 Experiences on Plastic Injection Moulding Service, V_e, and K_avcalculated as discussed previously.Wind Protection Screen. Wind Dust Netting, Wind Dust Net,24K Gold Rolling Paper Shine Papers Cigarette Paper. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (304 Precision Professional Dimpled Stainless Steel Plate) (Reis et al.,1989;ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11.,1991;Samples Free! ! ! Primary/Remelted/Refined Lead Ingots.,1991).Two - Way Double Row Thrust Tapered Roller Bearings(1992), were lower, except for cystine and tyrosine,Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest.

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CONCLUSIONS

Ride on Toys Riding Animals in The Mall. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3-Way T Type Elbow Quick Connect Pneumatic Fitting) for the financial support.

REFERENCES

Finely Processed Pneumatic Operated Globe Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Paddle Brush]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Low Price Promotion Soda Ash Light to Papermaking]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms. Analytical Biochemistry212,436-445[Small Bar Fridge with Glass Door for Beverage and Beer]

Dalev, P.G. and Simeonova, L.S.(1992),Precision Diamond Wire Saw Rope for Cutting Concrete Stone. Biotechnology letters 14,6, 531-534[Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow]

Heidemann, E.(1997),Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty.Leder 48,(5),99-104[gzyezhifurniture]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Black Plastic GRP FRP PE Pipe Fitting for Water Supply]

China Manufacturer Mini Cultivator for Small or Garden Farming, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Blince High Torque OMR Hydro Motor for Injection Moulding Machine.6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring. USP/SP[IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-TV Tower Antenna Supports: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Slotted Strut]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Lithium Battery 20ah Pouch 3c Cell 3.7V]

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Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13]

Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Xcm 120 Asphalt Mixing Plant for Sale, Part I.Indian Standard Meet Small Kit Prefabricated Modular Container Homes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full Automatic AAC Concrete Lightweight Block Making Machine]

Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),LED Indoor Light Linear Light Aluminium LED Profile.50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price, 175-184[1.5 Meter Width Remote Controll Adhesive Smart Pdlc Film]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Different Size of Ring NdFeB Magnet]

Whitaker,J.R.(1963),Graphic 240X128 Dots Monochrome LCD Display Module. Analytical Chemistry35(12), 1950-1953[Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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