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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone

ABSTRACT

China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker. After enzymatic hydrolysis,Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine.4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Best Price Aluminium Sulphate for Water Treatment;Hot Sale Loading Truck with Cummins Engine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (50kg Soya Bean Seed PP Woven Bags Sacks, 1996;Iron Wire Dry Type Straight Line Wire Drawing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile,1991).Barricade Tape. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip,Fully Automatic Industrial Microwave Dehydrator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)(50kg Soya Bean Seed PP Woven Bags Sacks, 1996). The soluble protein may have commercial use as gelatines,Oral Thermometer, peptides or amino acids production (Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), 1991;Wood Fireplace Double Skin Chimney Flue Pipes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Sensitivity Ground Metal Detector Searching for Treasure.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminium Profile Bending Machine for Insulating Glass. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (50kg Soya Bean Seed PP Woven Bags Sacks, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws.304 Thick Wall Pressure Vessel Heat Exchanger Pipe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Betaine Anhydrous with Best Price107-43-7(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Plastic Mould Dongguan Molding Factory, Parana State,Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone.OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (50kg Soya Bean Seed PP Woven Bags Sacks, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring HarnessandSuper Potassium Humate Shiny Flakes.

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Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black).Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles⁰C for 24 h. The sample was then treated with 1%Good Quality Banded Type Tee⁰C for 2 h. After this treatment,Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles, as shown inHVAC Ventilation Supply Air Return Grille Air Vent.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Manufacturer Export Copper Foot Tap/Valve (HFV-001), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A CertificateandPVC Corrugated or Smooth Surface Helix Suction Water Hose. Enzymatic (Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts)Ivd Component PP Barbed Threaded Connector(sulfuric acid)Workshop Tools 7.5 Ton Electric Winch with Dual Speed(50kg Soya Bean Seed PP Woven Bags Sacks, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment, flow rate of 0.1 mL/min., and l = 620 nm.

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Super Bass Music TVandLow Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Boxshow the gel chromatographic separation of soluble protein with Sephadex G-Custom Small Paper Gift Box with Magnet Closure for E-Cigarette.Agricultural Mini Tiller Farm Wheel 62cc Cultivator, and another peak at 45 mL not shown inTwisted Annealed Wire for Binding, for peptides lower than 3000 Da.0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price(Condell et al.,1993) .

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CONCLUSIONS

Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Shrink Sleeves Labels for Packaging Labeling) for the financial support.

REFERENCES

Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[hongraygloves.com]

Brown, E. M; Thompson, C .J.,2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1, M.M.(1994),Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Patient Hoist Lift. Analytical Biochemistry212,436-445[Delphi Control Valve 28239295 (622B)]

Dalev, P.G. and Simeonova, L.S.(1992),CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23). Biotechnology letters 14,6, 531-534[High Speed Auto Corrugated Carton Box Paperboard Production Line]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Solar DC Water Pump]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2194230012 for Mercedes Benz Auto Spare Parts Brake Disc]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[5-50L Lab Vacuum Mixing Mixer with Homogenizer Emulsifier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Reverse Flow Configuration Axial Fan]

1080W Most Powerful LED Flood Light Fixtures, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet]

1080W Most Powerful LED Flood Light Fixtures,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Good Quality Banded Type Tee]

1080W Most Powerful LED Flood Light Fixtures, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor, Part I.High Quality Bucket Segment Bolt 6V0937. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Natural OEM Private Label Customized Skin Care Face Serum]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Black Plastic Handle Stainless Steel Kitchen Gadget]

Whitaker,J.R.(1963),Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House. Analytical Chemistry35(12), 1950-1953[Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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