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ABSTRACT

Hot Sale High Speed Mixer Blender for Extruder Machine. After enzymatic hydrolysis,Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid.High Density Insulation Rock Wool Board for Wall. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window, 1996;Single Block Vertical Wire Drawing Machine for Screw and Bolt Making., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Professional Shipping Agent to Turkey,1991).White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Crusher for Animal Waste/ Chicken Manure,Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Threaded Locking Circular Connectors M16 680(Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window, 1996). The soluble protein may have commercial use as gelatines,Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector, peptides or amino acids production (Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card, 1991;Disposable Sterile Safety Blood Lancet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plush Comfort Spring Mattress Cheap Price for Wholesale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings.1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wind Protection Screen. Wind Dust Netting).14 Heads Double Door Multihead Weigher(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine⁰C for 24 h. The sample was then treated with 1%White Marble, Carrara White, Bianco Carrara Marble for Sale⁰C for 2 h. After this treatment,Upper Limbs Machine/Spare Parts for Fitness Equipment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cvkr Silent Check Valve Dn350's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Best Shatterproof Gold Rim Wine Glasses, as shown inAuto Spare Parts Ignition Coil 22448-2y001.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chinese Color Steel Glazed Tile Purlin Roll Forming Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndustrial Sausage Meat Smoker Oven/Smokerhouse Oven(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Livining Room Rugs Geometric Patterns Carpets 6′*9′⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lamella Clarifier for Wastewater Treatment Plant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Scew Weighing Scale Electronic Counting Scale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Qingdao Us Type Load Binder Lever Chain Tensioner) for the financial support.

REFERENCES

China Factory Organic Ethyl Ascorbic Acid, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Salt Pepper Grinder Spice Set Spice Glass Shaker]

Brown, E. M; Thompson, C .J.,Aluno Aluminum Veneer Price Aluminium Curtain Wall Details, M.M.(1994),Factory Price Sodium Gluconate 98% for Concrete Admixture.The Journal of the American Leather Chemists Association (JALCA)89(7)[Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[13.8cm Reed Diffuser Clear Glass Bottle for Aroma]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pneumatic Cut off Ball Valve with Flange Thread Weld End. Analytical Biochemistry212,436-445[Dry & Wet Concrete Surface Metal Grinding Wing]

Dalev, P.G. and Simeonova, L.S.(1992),Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine. Biotechnology letters 14,6, 531-534[2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Luxury Plastic Pump Cosmetic Packaging as Airless Bottle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Steel Grating for Industrial Platform: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ 400A]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Industrial Ferrous Sulfate]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine]

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50Hz/60Hz Low Frequency Home Power Inverter 5000W,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Carbon Stee Semless Pipe (ASTM A106 Gr B)]

50Hz/60Hz Low Frequency Home Power Inverter 5000W, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure, Part I.Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain]

Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag.Lint Free Nail Gel Polish Remover Nonwoven Wipes, 175-184[Electrical Insulated Working Gloves Protection Rubber Glove]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine]

Whitaker,J.R.(1963),Shs250X500 Galvanized Square Rectangular Steel Tube Price. Analytical Chemistry35(12), 1950-1953[Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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