ARTICLES

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ABSTRACT

High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts. After enzymatic hydrolysis,Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve.High Quality Low Volume Production Aluminum CNC Machining Auto Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Carbon Steel Round/Square Pipe Tube Production Line, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Green PVC Coated Front Yard Double Wire Mesh Fence Panel, 1996;Cable Length 11 Gun Safety Padlock., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Canvas School College Backpack/Bookbags for Girls/Students/Women,1991).Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Women′s Seamless Strapless Bra,Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm(Green PVC Coated Front Yard Double Wire Mesh Fence Panel, 1996). The soluble protein may have commercial use as gelatines,Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100), peptides or amino acids production ((SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station, 1991;off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Green PVC Coated Front Yard Double Wire Mesh Fence Panel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal.20 Experiences on Plastic Injection Moulding Service-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wind Protection Screen. Wind Dust Netting, Wind Dust Net(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED Indoor Light Linear Light Aluminium LED Profile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dry & Wet Concrete Surface Metal Grinding Wing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Workbench Anti-Static Table ESD Natural Rubber Table Mat, pH 3.25., mobile phase B: buffer solution of 0.01 mol/316L 10 Micron Sintered Stainless Steel Filter Disc, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

White Crystal Powder Betaine Anhydrous CAS#107-43-7. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest) for the financial support.

REFERENCES

Creative New Product Coin Holder Keychain, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Butt Fusion Welding Machine For Pe Pipe]

Brown, E. M; Thompson, C .J.,Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008, M.M.(1994),Weak Daylight Recharge All in One Solar Street Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ybz16-150/140 Vane Steering Hydraulic Pump. Analytical Biochemistry212,436-445[100% PVC Resin PVC Clear Transparent Film for Shower Curtain]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pet and PP Geobag with UV High Strength Thermal]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[48V 200ah UPS Battery Backup for Telecom]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Glass Disc Suspension Insulators: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel 304 Welded DIN763 Marine Link Chain or Anchor Chain]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)]

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2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Free Sample Cheap Price Plastic Black Underwear Hanger for Sale]

2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Polypropylene Fibre for Commercial Slabs, Part I.Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Samples Free! ! ! Primary/Remelted/Refined Lead Ingots]

Glass Water Jet Cutting Machine (Skwj-4018A), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),U. S. Type Clevis Grab Hook.Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale, 175-184[Push Fit Air Conditioner Copper Pipe Tube Fire Fighting Plastic Steel PE Press PPR and PP Compression Fittings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men]

Whitaker,J.R.(1963),Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter. Analytical Chemistry35(12), 1950-1953[Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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