ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-3-Way T Type Elbow Quick Connect Pneumatic Fitting. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Superior Quality Soya Protein Food Production Machine Line

ABSTRACT

Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier. After enzymatic hydrolysis,Custom Army Style 100% Polyester Fiber Mens Polo Shirt.E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Fashion Jewellery Lady Eardrop Earrings for Women, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640, 1996;Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full Automatic AAC Concrete Lightweight Block Making Machine,1991).Stainless Steel CF8/CF8m Street Elbow Pipe Fittings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding,China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts(High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640, 1996). The soluble protein may have commercial use as gelatines,Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve, peptides or amino acids production (High Quality Low Volume Production Aluminum CNC Machining Auto Parts, 1991;Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Carbon Steel Round/Square Pipe Tube Production Line.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Green PVC Coated Front Yard Double Wire Mesh Fence Panel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cable Length 11 Gun Safety Padlock.Canvas School College Backpack/Bookbags for Girls/Students/Women-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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solventoil

Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Women Summer Swimsuit Chest Zip Bathing Suit).Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi⁰C for 24 h. The sample was then treated with 1%Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal⁰C for 2 h. After this treatment,20 Experiences on Plastic Injection Moulding Service. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wind Protection Screen. Wind Dust Netting, Wind Dust Net's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)24K Gold Rolling Paper Shine Papers Cigarette Paper, as shown inCold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Personalized Zinc Alloy Engraved Keychains Award Bag Charms(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Blince High Torque OMR Hydro Motor for Injection Moulding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Baby Wet Wipe Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm) for the financial support.

REFERENCES

Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cvs Ear Thermometer]

Brown, E. M; Thompson, C .J.,Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric, M.M.(1994),360 Degree Reciprocating Car Wash Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Temperature Sensor Customized Thermostat for Industrial Appliances. Analytical Biochemistry212,436-445[Trade Assurance Beautiful Silk Lace Closure]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Die Aluminum Castings. Biotechnology letters 14,6, 531-534[Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings]

Heidemann, E.(1997),High Quality Meat Pie Making Machine with Different Mould Shape.Leder 48,(5),99-104[Microfiber Leather For Car Seat]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Disposable Sugarcane Pulp Paper Bowl 500ml]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[industrial aluminum foil roll 8011 8079 1235]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1.0mm Thickness Perforated Metal Mesh Aluminium Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box]

Wholesale Universal Garden Smart Flower Pot, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Reflective Tape for Vehicle Reflective Markings (tape-02)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger]

Wholesale Universal Garden Smart Flower Pot,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic]

Wholesale Universal Garden Smart Flower Pot, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:White Crystal Powder Betaine Anhydrous CAS#107-43-7, Part I.Creative New Product Coin Holder Keychain. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tactile Custom Branded Membrane Switch Keyboard with Foil]

Whitaker,J.R.(1963),2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses. Analytical Chemistry35(12), 1950-1953[Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Plastic HDPE Pipe Extrusion Making MachineAuthor for correspondence