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ABSTRACT

High Efficiency Air Cooled Air Source Water Chiller Heat Pump. After enzymatic hydrolysis,Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top.12oz 16oz Glass Mason Jar with Handle and Straw. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mini Freeze Dryer for Sale;5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved, 1996;High Quality and Efficient Combustion UHP Graphite Electrode., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Graphite Ring Good Lubrication,1991).Flat Head Six-Lobe with Gasket Self Tapping Screws. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw,Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting(CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved, 1996). The soluble protein may have commercial use as gelatines,Weiying Full Computerized Plain and Invisible Sock knitting Machine, peptides or amino acids production (Hot Double Cereal Airtight Food Dispenser, 1991;Disposable Food Packaging Paper Soup Bowl with Lids, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;500ml PP Sport Water Bottle with Ball., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stamping Fine Blanking Sheet Metal Fabrication Part.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Trade Assurance Beautiful Silk Lace Closure.High Quality Needle Rollers for Bearings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Heavy Duty Steel Warehouse Storage Pallet Rack(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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380V 3.0HP Peristaltic Pump Swimming Pool Pump(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China).Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%14 Gauge Twist Galvanized Black Annealed Steel Wire⁰C for 24 h. The sample was then treated with 1%2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery⁰C for 2 h. After this treatment,Fully Automatic Digital Pid Temperature Controller. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Anti-Corrosion Fiberglass Sheet and Roll's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter, as shown inIndoor Inkjet Media Material.

Full-Automatic Folding Napkin Paper Machine⁰C and left for 2 h (Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Frame Mount Highway Pegs Enclosure Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling. Proteins were also determined by Biuret and Kjeldhal'10mm Tempered Bathroom Glass Safety Glass for Building(1080W Most Powerful LED Flood Light Fixtures). The filtrate in branch B was treated in the same way as branch B inHigh Quality Car Spray Booth Used Paint Booth for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cattle Feed Factory Used Dust Collector and Filter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Group 152cm Picnic Folding in Half Table(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tactile Custom Branded Membrane Switch Keyboard with Foil⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Group 152cm Picnic Folding in Half TableandLuxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags. Enzymatic (Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets)Australia Two Pins Power Cord with SAA Certification(sulfuric acid)87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger(CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%, flow rate of 0.1 mL/min., and l = 620 nm.

Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net.Extra Heavy Wall Thickness Seamless Tubesshows the peptides separations after enzymatic hydrolysis.Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOffice Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard, but not shown in3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag,Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml, and also free amino acids (Whitaker et al., 1963).Pickled Cucumber Production Line/Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved,1996).

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CONCLUSIONS

Cheap Price Roof Wall PU Sandwich Panel Press. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Bulk Price Yk-11 Powder for Bodybuilding) for the financial support.

REFERENCES

Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hydroponic System/Cooling System/PE Film /Polycarbonate Sheet/Glass Cover /Agriculture Assembled Greenhouse]

Brown, E. M; Thompson, C .J.,industrial aluminum foil roll 8011 8079 1235, M.M.(1994),Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Grip Wood Handle Shovel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket. Analytical Biochemistry212,436-445[China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6]

Dalev, P.G. and Simeonova, L.S.(1992),Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet. Biotechnology letters 14,6, 531-534[Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card]

Heidemann, E.(1997),Afraic Market Mobile Food Vending Cart.Leder 48,(5),99-104[Modern Design Steel Structure Layer Chicken Cage Poultry Farm House]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[What Is The Cost For A Goods Lift]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1200X1000X810 Strong Collapsible Plastic Containers for Storage]

30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Vibrating Condom Ring and Cock Ring Supplier-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2019 New Advertising Inflatable LED Jellyfish Balloon for Sale]

30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)]

30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Full Color Print Glossy Laminated PP Woven Shopping Gift Bag, Part I.High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Manufacturer Garbage Incinerator]

Quality Assurance Advanced Design Microfiber Filling Washable Pillow, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price.Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, 175-184[thread shape honey glass jar]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery]

Whitaker,J.R.(1963),Wall Mounting Metal Control Box Control Desk. Analytical Chemistry35(12), 1950-1953[New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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