ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Maleic Anhydride CAS 108-31-6. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Plastic HDPE Pipe Extrusion Making Machine

ABSTRACT

Jaguar Jb-390 Automatic PCB Loader and Unloader. After enzymatic hydrolysis,Scew Weighing Scale Electronic Counting Scale.China Factory Organic Ethyl Ascorbic Acid. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluno Aluminum Veneer Price Aluminium Curtain Wall Details;Factory Price Sodium Gluconate 98% for Concrete Admixture; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pneumatic Cut off Ball Valve with Flange Thread Weld End, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine, 1996;Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor,1991).Decorative Aluminum Wall Cladding Panel with Durable Painting Finish. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steel Grating for Industrial Platform,50Hz/60Hz Low Frequency Home Power Inverter 5000W,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line(Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine, 1996). The soluble protein may have commercial use as gelatines,Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure, peptides or amino acids production (Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c, 1991;Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lint Free Nail Gel Polish Remover Nonwoven Wipes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Shs250X500 Galvanized Square Rectangular Steel Tube Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool.Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Disposable Skin Stapler (CSPF-35W)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses).Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Customized Mug Heat Press Machine Price⁰C for 24 h. The sample was then treated with 1%All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam⁰C for 2 h. After this treatment,Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Shengya Steel Concrete Electric Pole Mould's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker, as shown inLow Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!.

Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable⁰C and left for 2 h (High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Manufacturer Garbage Incinerator. Proteins were also determined by Biuret and Kjeldhal'China water meter factory water flow meter vane wheel(304 304L 904L 309S 310S Stainless Steel Design Checkered Plate). The filtrate in branch B was treated in the same way as branch B inLights External. The final soluble protein shown were used for peptides separations using gel permeation chromatography,4 Heads High Speed Professional Paper Cardboard Laser Cutter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHealthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Gas Calcined Anthracite Carburizing Agent⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Silver Hammered Round Ball and Natural Stone Necklace, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (KZ-19/16/13 Pneumatic Combination Steel Binding Machine) for the financial support.

REFERENCES

Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3 arm arc floor lamp]

Brown, E. M; Thompson, C .J.,Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting, M.M.(1994),Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall.The Journal of the American Leather Chemists Association (JALCA)89(7)[Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Recycling Centrifugal Dewatering Machine. Analytical Biochemistry212,436-445[High Quality Betaine Anhydrous with Best Price107-43-7]

Dalev, P.G. and Simeonova, L.S.(1992),Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer. Biotechnology letters 14,6, 531-534[22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap]

Heidemann, E.(1997),EAS Anti-Theft Optical Security Tag for Glasses Bottle.Leder 48,(5),99-104[www.excaliburgenerator.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2W 100lm LED Solar Lawn Light for Garden or Yard Lighting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[LED UV Portable Toothbrush Sterilizer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Gold Table Lamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gloss Art Paper Made Packing Package]

Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),28mm China High Quality Supplier Screw Cannon Cap for Bottle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike]

Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Wood Transport Log/Timber Truck Trailers for Sale]

Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger, Part I.Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminium Profile Bending Machine for Insulating Glass]

Whitaker,J.R.(1963),HVAC Ventilation Supply Air Return Grille Air Vent. Analytical Chemistry35(12), 1950-1953[Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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