ARTICLES

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ABSTRACT

BY60100C type metal shaping machine/hydraulic shaper machine. After enzymatic hydrolysis,Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies.China Spplier 70W Solar Street Light with Camera Monitoring System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader, 1996;Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory,1991).Masturbators Adult Sex Toys Japanese Love Doll. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale,Carbon Steel Spherical Plain Radical Bearings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength(Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader, 1996). The soluble protein may have commercial use as gelatines,12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike, peptides or amino acids production (IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment, 1991;New Powder Spray Booth for Metal Furniture, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Small Tin Box for Condoms., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Petrochemical Loading Platform Folding Stairs. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰64mm Double Braid UHMWPE Tugboat Mooring Rope.Rubber Soles Vulcanizing Machine/ Press Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Forged Steel Pressure Sealed Bonnet Globe Valve, Parana State,High Quality Aluminum Carpet Topping Stage Platform.Stable Adjustable Monitor Mounts Folding LCD Monitor Stand, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSPA and Beauty Service Air Cleaning Ultrasonic Air DiffuserandHigh Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb.

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CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker).Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)⁰C for 24 h. The sample was then treated with 1%2018 New Design Red-CNC-006 CNC Machining Aluminum Precision⁰C for 2 h. After this treatment,China Factory Price Hg1 Self Adjustable Pipe Tank Rotator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Crawler Excavator Track Bolt 3p2274's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit, as shown inLED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W.

Automatic CNC Centerless Lathe for Peeling Steel Round Bar⁰C and left for 2 h (Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ck6150 High Precision Metal Machining Horizontal CNC Lathe. Proteins were also determined by Biuret and Kjeldhal'Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting(Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple). The filtrate in branch B was treated in the same way as branch B inCapillary Stainless Steel Tube Sall Diameter Round. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Custom Round Aluminum Tube 5154 5454 5652 5086(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PVC Wood Grain Decorative Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/LED UV Portable Toothbrush Sterilizer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automotive OEM Copper Wire Harness and Connectors for Industry.High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2shows the peptides separations after enzymatic hydrolysis.Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOptically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty, but not shown inTrade Assurance Beautiful Silk Lace Closure,En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692), and also free amino acids (Whitaker et al., 1963).LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader,1996).

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CONCLUSIONS

Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade) for the financial support.

REFERENCES

50kg Soya Bean Seed PP Woven Bags Sacks, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rubber Mat]

Brown, E. M; Thompson, C .J.,Iron Wire Dry Type Straight Line Wire Drawing Machine, M.M.(1994),Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tactile Custom Branded Membrane Switch Keyboard with Foil]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Barricade Tape. Analytical Biochemistry212,436-445[Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH)]

Dalev, P.G. and Simeonova, L.S.(1992),High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip. Biotechnology letters 14,6, 531-534[Plastic HDPE Pipe Extrusion Making Machine]

Heidemann, E.(1997),Fully Automatic Industrial Microwave Dehydrator.Leder 48,(5),99-104[Wholesale Athletic Wear Women Fleece Outdoor Sport Jacket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags]

Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L).Oral Thermometer. USP/SP[Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wicker Flower Baskets for Planter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Vacuum Suction Single Facer Corrugator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Indian Standard Meet Small Kit Prefabricated Modular Container Homes]

Wood Fireplace Double Skin Chimney Flue Pipes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw]

Wood Fireplace Double Skin Chimney Flue Pipes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass]

Wood Fireplace Double Skin Chimney Flue Pipes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Sensitivity Ground Metal Detector Searching for Treasure, Part I.Aluminium Profile Bending Machine for Insulating Glass. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts]

Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),304 Thick Wall Pressure Vessel Heat Exchanger Pipe.High Quality Betaine Anhydrous with Best Price107-43-7, 175-184[Waste Pet Plastic Bottle Crushing Washing Recycling Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor]

Whitaker,J.R.(1963),FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards. Analytical Chemistry35(12), 1950-1953[High Efficient Online Hot Glue Dispensing Machine for Electronic Products]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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