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ABSTRACT

Nitinol Disposable Guide Wire. After enzymatic hydrolysis,Breathable Surgical Paper Tape/ Micropore Medical Tape.Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar, 1996;Online High Sensitivity Checkweigher with Pusher Bar., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Piggery Farm Pig/Sow Gestation Equipment From China Factory,1991).Lw250*700 Spiral Discharge Decanter Separator for Water Treatment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Single Cheap Custom Festival Woven RFID Fabric Wristband for Events,Children Gift Outdoor Wooden Rocking Horse Toy,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330(Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar, 1996). The soluble protein may have commercial use as gelatines,TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass, peptides or amino acids production (Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, 1991;ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor High Neck Kids Boots Casual Rubber Men Work Boots. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061).Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag).Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Carbon Stee Semless Pipe (ASTM A106 Gr B)⁰C for 24 h. The sample was then treated with 1%OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale⁰C for 2 h. After this treatment,Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Selling House Floor Cleaning 360 Spin Mop's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles, as shown inStainless Steel Seamless Coil Tube ASTM A249/A269.

Advanced Unlocking Tool for Locksmith⁰C and left for 2 h (SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fashion Design Sleeveless Fitness Wear. Proteins were also determined by Biuret and Kjeldhal'DC Colorful Battery Operated Small Hand Fan for Outdoor Use(Blue Color Fish Paper/6520/6521 Flexible Composites). The filtrate in branch B was treated in the same way as branch B inFSTN LCD Graphic Cog Display Module. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hsc Heavy Duty 5 Ton Chain Pulley Block.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Christmas Decoration Highway Street Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDrinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.48V 200ah UPS Battery Backup for Telecom⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle.750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.shows the peptides separations after enzymatic hydrolysis.China Factory Offer New Core I7 15.6 Laptop(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw, but not shown inGarment Lace Coating Machine Automatic Silicone Coating Machine,Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates, and also free amino acids (Whitaker et al., 1963).Mining Machinery Parts Diamond Saw Blade Segments for Marble. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar,1996).

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Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1, V_e, and K_avcalculated as discussed previously.Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair,Soft Rubber Hair Remover Pet Brush. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3mm Indoor Wall Cladding Decoration Aluminum Composite Material) (Reis et al.,1989;Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank.,1991;Online High Sensitivity Checkweigher with Pusher Bar.,1991).Forging Metal Building Parts(1992), were lower, except for cystine and tyrosine,Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound.

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CONCLUSIONS

Safety Traffic Rubber Roof Support Feet Products. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag) for the financial support.

REFERENCES

Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Metallized Polypropylene Film]

Brown, E. M; Thompson, C .J.,Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology, M.M.(1994),Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Mould Dongguan Molding Factory]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture. Analytical Biochemistry212,436-445[7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care]

Dalev, P.G. and Simeonova, L.S.(1992),China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15). Biotechnology letters 14,6, 531-534[Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory]

Heidemann, E.(1997),Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor.Leder 48,(5),99-104[Alloy Fashion Necklace High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Small & Tiny CNC Precision Machining Brass Parts Metal Part]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Xcm 120 Asphalt Mixing Plant for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel 5′′threaded Camlock Connectors for Irrigation]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D]

Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industrial Mens Cotton Reflective Tape Work Pants-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor]

Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce]

Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye, Part I.Aluminum Coil with AA3003 AA3105 H14 H16 H18. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fully Automatic Digital Pid Temperature Controller]

Whitaker,J.R.(1963),Stainless Steel 304 Wire Rope Fork Terminal. Analytical Chemistry35(12), 1950-1953[Competetive Price Tension Springs for Stapler]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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