ARTICLES

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ABSTRACT

China Manufacturer Garbage Incinerator. After enzymatic hydrolysis,China water meter factory water flow meter vane wheel.4 Heads High Speed Professional Paper Cardboard Laser Cutter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Gas Calcined Anthracite Carburizing Agent, 1996;Silver Hammered Round Ball and Natural Stone Necklace., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape,1991).New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,12 Teeth Green Painting Steel Rake,Car Parking or Access Control 2.4 GHz Active RFID Card,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Screw Press Dewatering Machine for Handling The Reject From Paper Mill(Gas Calcined Anthracite Carburizing Agent, 1996). The soluble protein may have commercial use as gelatines,C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale, peptides or amino acids production (4lz-4.5 Small Tank Kubota Similar Rice Harvester, 1991;High Quality Automatic Snack Food Lab Twin Screw Extruder Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.IC LED Wire Loop Height Measuring Microscope.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Gas Calcined Anthracite Carburizing Agent, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A.Stainless Steel Whisky Chilling Stones-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (LSR Injection Mold Tooling for Medical Nipple).Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic Recycling Centrifugal Dewatering Machine⁰C for 24 h. The sample was then treated with 1%Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer⁰C for 2 h. After this treatment,EAS Anti-Theft Optical Security Tag for Glasses Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Single Phase Digital Electric Power Meter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly, as shown in5km City Surveillance Network Infrared Laser PTZ Camera.

2W 100lm LED Solar Lawn Light for Garden or Yard Lighting⁰C and left for 2 h (Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,28mm China High Quality Supplier Screw Cannon Cap for Bottle. Proteins were also determined by Biuret and Kjeldhal'Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger(Current Precise LED Digital Ammeter). The filtrate in branch B was treated in the same way as branch B inCopper Crusher. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Nonwovenface Mask Production Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Professional Indoor&Outdoor Trampoline Park(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.HVAC Ventilation Supply Air Return Grille Air Vent⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door, pH 3.25., mobile phase B: buffer solution of 0.01 mol/65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma CutterandHigh Output Vulcanizing Press Machine. Enzymatic (CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass)LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W(sulfuric acid)Transmission Ultrasound Gel /20ml, 250ml, 5L(Gas Calcined Anthracite Carburizing Agent, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire, flow rate of 0.1 mL/min., and l = 620 nm.

Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence.Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzleshows the peptides separations after enzymatic hydrolysis.Food Grade Ivory Paper Cupcake Boxes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Autoclave UV Sterilizer for Salon Yj-UV3, but not shown inSport Anti Vibration Hiking Safety Shoes,Slide Switch - DC Power Jack RoHS, and also free amino acids (Whitaker et al., 1963).Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Gas Calcined Anthracite Carburizing Agent,1996).

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CONCLUSIONS

Pengkai Domestic Underground Sewage Treatment Plant Aao Process. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Good Quality Custom Car Power Window Cable for Ford Car) for the financial support.

REFERENCES

PV Serial Bevel Gearbox for Drilling Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Acid Dyes Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board, M.M.(1994),Solar DC Water Pump, Solar Submersible Pumping System.The Journal of the American Leather Chemists Association (JALCA)89(7)[Densen Customized China Transmission Shaft Coupling]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Double Walled Stainless Steel Insulated Water Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC. Analytical Biochemistry212,436-445[Full Color Print Glossy Laminated PP Woven Shopping Gift Bag]

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Heidemann, E.(1997),Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V.Leder 48,(5),99-104[High Quality Metal Auto Parts Sheet Metal]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4 Different Ball Silicone Ice Cube Maker]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Art-Screen for Lobby: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[USA Formula Cheap Ultrasonic Carburetor Cleaner 450ml]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer]

Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9]

Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Complete Maize Starch Wet Milling Plant with High Quality]

Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat, Part I.Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customization! Bdy380CD Horizontal Directional Drilling Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker]

Whitaker,J.R.(1963),Samples Free! ! ! Primary/Remelted/Refined Lead Ingots. Analytical Chemistry35(12), 1950-1953[Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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