ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Electric Winch With Groove Drum. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump

ABSTRACT

Truck Air Dryer cartridge 14bar. After enzymatic hydrolysis,Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors.3mm White Waterproof PVC Foam Board. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Women Party Jewelry 45cm Sequins Choker, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity, 1996;High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Freqency PCB with Blind Via,1991).Strong Aluminum Armrest Reclining Hydraulic Barber Chair. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory SMD3528 60LEDs/M Flexible LED Strip,Hot-Press Small Continuous Diamond Saw Blade Cutting Disc,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage(New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity, 1996). The soluble protein may have commercial use as gelatines,Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick, peptides or amino acids production (Comfort Furniture Natural Spring Memory Foam Mattress, 1991;Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4.2A Type a Type C Pd QC3.0 USB Charger., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ferrous Sulphate Monohydrate 30%Min Factory Wholesale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Small Portable Scooters Folding Electric Scooters for Adults. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant.DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Horizontal Window Shade Blind Zebra Dual Roller Blinds Day).RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles⁰C for 24 h. The sample was then treated with 1%Cog Mono Round Display LCD Module⁰C for 2 h. After this treatment,4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Products Suppliers Camlock Coupling Quick Couplings Female, as shown inEco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPrime Gi Steel Strip Galvanized Metal Strip Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

OEM High Pressure Machined Part Die Aluminum Casting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine) for the financial support.

REFERENCES

AISI 430 Ba/2b Embossed Stainless Steel Sheet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fecal Occult Blood Sample Tube Suppliers for Sale]

Brown, E. M; Thompson, C .J.,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, M.M.(1994),Drug Delivery ABS Hospital Medical Treatment Medicine Cart.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2070 4th Axis CNC]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black. Analytical Biochemistry212,436-445[China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net]

Dalev, P.G. and Simeonova, L.S.(1992),Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress. Biotechnology letters 14,6, 531-534[Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected]

Heidemann, E.(1997),Colorful Electric English Funny Music Story Module Printing Book.Leder 48,(5),99-104[Pigtail Fastener Supplier & Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor Christmas Decoration Highway Street Light]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Single Flute End Mill Milling Cutter Tools for Wood Milling]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tilt-and-Turn-with-Hidden-Hinge: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized High Quality Cast Iron Manhole Cover]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Graphic 240X128 Dots Monochrome LCD Display Module]

Automatic Soft PVC Granule Used for Refrigerator Door Gasket, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),0.45um Dia 47mm PVDF Filter Membrane-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Power Extension Cord AC Cable Plug for Office Equipment]

Automatic Soft PVC Granule Used for Refrigerator Door Gasket,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts]

Automatic Soft PVC Granule Used for Refrigerator Door Gasket, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Commecial Use Watch Display Cabinet for Watch Store Interior Design, Part I.Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2018 New Style High Quality Enamel Bowl with Lid]

Slotted Round Head Aluminum Self Tapping Screw, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike.Classic Black Color Commercial Glass Door Multi Deck Refrigerator, 175-184[Cnc Frame For Milling Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager]

Whitaker,J.R.(1963),MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker. Analytical Chemistry35(12), 1950-1953[2018 Cheap PU Leather Bracelet in Alloy Jewelry]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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