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ABSTRACT

LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip. After enzymatic hydrolysis,Carbon Steel Equal Tee for Connection of Pipe.Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy;Steel Stacking Restaurant Wedding Chair; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rutile Anatase Titanium Dioxide for Coating and Paints, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Laser Cutting Machine for Sale for Die Shops From Yitai Die Making, 1996;Di003 Environmental Protection Against The Stench Floor Drain., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck,1991).Vacuum Type Single Facer Corrugator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken,OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve(Laser Cutting Machine for Sale for Die Shops From Yitai Die Making, 1996). The soluble protein may have commercial use as gelatines,125mm Wet Diamond Pad for Polishing Marble, peptides or amino acids production (Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter, 1991;40W Integrated Solar Street Lighting LED for Park, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Design Many Styles Fancy Wine Display Rack (6532).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Checkered Plate 304 316L 410 Floor Material. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Laser Cutting Machine for Sale for Die Shops From Yitai Die Making, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box.Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine, Parana State,Power Plant Circulating Water Pump & Spare Part.24W 400W Rainproof Switch Power Adapter for LED Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Laser Cutting Machine for Sale for Die Shops From Yitai Die Making, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Dental Primary Molar Crown Stainless Steel Kids CrownsandCompact New Design IP65 Toggle Limit Switch.

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Industrial Threaded Plastic Connector Female Electrical Plug(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Carbon Steel Equal Tee for Connection of Pipe).Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity⁰C for 24 h. The sample was then treated with 1%Geotextile for Airport Runways/High Way, Railway⁰C for 2 h. After this treatment,Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cute Cartoon Coin Purse Children Zipper Purse, as shown inRutile Titanium Dioxide Exterior Emulsion Paint.

Garment Lace Coating Machine Automatic Silicone Coating Machine⁰C and left for 2 h (Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tamper Evident Security Seals Heat Resistant Electronic Tape. Proteins were also determined by Biuret and Kjeldhal'South Africa Standards Solar Power Variable Message Sign LED Traffic Sign(Stainless Steel Hole Punching Machine/ CNC Perforation Machine). The filtrate in branch B was treated in the same way as branch B inRain Downspout Pipe Roll Forming Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avParty Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Complete Maize Starch Wet Milling Plant with High Quality⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Top Furniture Cheap Iron Stacking Banquet Hall Chairs, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mosquito Proof Fiberglass Fly Screen.China Factory Offer New Core I7 15.6 Laptopshows the peptides separations after enzymatic hydrolysis.Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable, but not shown inHeater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A,Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board, and also free amino acids (Whitaker et al., 1963).19m Mobile LED Display Billboard for Advertising Screens. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Laser Cutting Machine for Sale for Die Shops From Yitai Die Making,1996).

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Cooling Tower Replaced Fill Media, V_e, and K_avcalculated as discussed previously.A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow,Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370) (Reis et al.,1989;Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter.,1991;Di003 Environmental Protection Against The Stench Floor Drain.,1991).Stainless Steel Expanded Mesh(1992), were lower, except for cystine and tyrosine,Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2.

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CONCLUSIONS

Lf + Hf Contactless Smart Hybrid Card. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sport Anti Vibration Hiking Safety Shoes) for the financial support.

REFERENCES

Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.vendingmachinechina.net]

Brown, E. M; Thompson, C .J.,LED Fairy Star Light for All Kinds of Decoration Skylike, M.M.(1994),Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply.The Journal of the American Leather Chemists Association (JALCA)89(7)[Screw Press Dewatering Machine for Handling The Reject From Paper Mill]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Linear Vibrator Screener, for Granule and Powder Selection and Classification. Analytical Biochemistry212,436-445[Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders]

Dalev, P.G. and Simeonova, L.S.(1992),Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket. Biotechnology letters 14,6, 531-534[Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet]

Heidemann, E.(1997),Children Hardcover Book Printing Services with Free Printing File Checking.Leder 48,(5),99-104[NSK NTN Koyo Precision High Speed 6206 6207 6208 6210 Zz C3 Bicycle Motor Deep Groove Ball Bearing 6201 6202 6203 6204 6205]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card]

Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid.Global Brands 10 Year Cheaper Hook & Loop Tape Male Side. USP/SP[High Quality Cheap Price Disposable IV Infusion Set with Precise Filter]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Female Header Conn Ptich 1.0mm Connector Btb]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wooden Queen/King Size Japan Sofa Bed G1162]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Multi-Touch Interactive White Board for Multi-Media Classroom]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Nitinol Disposable Guide Wire]

Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price]

Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price]

Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, Part I.Large Size Stone Aluminum Honeycomb Panel for Facade. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag]

Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging.Making Machine for EVA Soft Hose, 175-184[Custom Design Drawer Gift Paper Box High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Educational Equipment Whiteboard]

Whitaker,J.R.(1963),Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474. Analytical Chemistry35(12), 1950-1953[Graphite Ring Good Lubrication]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

AgrochemicalsAuthor for correspondence