ARTICLES

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ABSTRACT

24K Gold Rolling Paper Shine Papers Cigarette Paper. After enzymatic hydrolysis,Two - Way Double Row Thrust Tapered Roller Bearings.Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Color Coated Corrugated Galvanized Roofing Steel Plate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting, 1996;Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Precision Diamond Wire Saw Rope for Cutting Concrete Stone,1991).Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Blince High Torque OMR Hydro Motor for Injection Moulding Machine,6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,TV Tower Antenna Supports(Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting, 1996). The soluble protein may have commercial use as gelatines,Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring, peptides or amino acids production (Retractable Multi Core Cable Reel, 1991;New Xcm 120 Asphalt Mixing Plant for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Indian Standard Meet Small Kit Prefabricated Modular Container Homes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED Indoor Light Linear Light Aluminium LED Profile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price.Graphic 240X128 Dots Monochrome LCD Display Module-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dry & Wet Concrete Surface Metal Grinding Wing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003), Parana State,Wind Protection Screen. Wind Dust Netting, Wind Dust Net.Cheapest Container Living House/Office/Kiosk, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLeisure Popular Hotel Waiting Office Leather Sofa with Wooden ArmrestandPractical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts.

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Spin Centrifugal Demister Separator or Column Internal(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rectangular Lunch Tin Box with Handle).Baby Wet Wipe Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump⁰C for 24 h. The sample was then treated with 1%Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric⁰C for 2 h. After this treatment,360 Degree Reciprocating Car Wash Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Temperature Sensor Customized Thermostat for Industrial Appliances's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Die Aluminum Castings, as shown inBest Selling Sequin Pillows / Reversible Cushions Square (YFC0061).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Reflective Tape for Vehicle Reflective Markings (tape-02). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWhite Crystal Powder Betaine Anhydrous CAS#107-43-7(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Weak Daylight Recharge All in One Solar Street Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ybz16-150/140 Vane Steering Hydraulic Pump, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Factory Supply Industry Grade Sodium Gluconate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket) for the financial support.

REFERENCES

Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Capacitor Polyester Film]

Brown, E. M; Thompson, C .J.,En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube, M.M.(1994),Mini High Temperature Clothing Labels Screen Printing Machine Factory.The Journal of the American Leather Chemists Association (JALCA)89(7)[V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Women Summer Swimsuit Chest Zip Bathing Suit]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.. Analytical Biochemistry212,436-445[Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale. Biotechnology letters 14,6, 531-534[Finely Processed Pneumatic Operated Globe Valve]

Heidemann, E.(1997),Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts.Leder 48,(5),99-104[Commercial Led Pendant Lighting]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Precision Green Sand Casting Ball Valve Parts]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Car Parking or Access Control 2.4 GHz Active RFID Card]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ce Marked Medical Syringe Pump (WP08N): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.startexindustrial.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line]

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New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb]

New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera, Part I.Electro Galvanized Low Carbon Straight Cut Wire. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line]

Geotextile for Roofs, Civil Applications. Railway Work, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pneumatic Shuttle Valve Flange Ends.Stx825 Dual 15 Powerful PA Speaker, Speaker Box, 175-184[Waterproof Swim Trunks]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker]

Whitaker,J.R.(1963),Ce Approval Biscuit Trayless Packing Machine (MZ-250B). Analytical Chemistry35(12), 1950-1953[LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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