ARTICLES

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ABSTRACT

industrial aluminum foil roll 8011 8079 1235. After enzymatic hydrolysis,Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval.High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet;Afraic Market Mobile Food Vending Cart; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Double Wall Glass Coffee Mug Tea Cups, 1996;Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care,1991).Vibrating Condom Ring and Cock Ring Supplier. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Full Color Print Glossy Laminated PP Woven Shopping Gift Bag,High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Quality Assurance Advanced Design Microfiber Filling Washable Pillow(Double Wall Glass Coffee Mug Tea Cups, 1996). The soluble protein may have commercial use as gelatines,Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price, peptides or amino acids production (Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, 1991;Wall Mounting Metal Control Box Control Desk, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Precision Tooling Grinding Machine Part., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Kellogg′s Corn Flakes Cereals Snacks Food Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Double Wall Glass Coffee Mug Tea Cups, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid.Various Hose and Output Optional Hose Squeeze Pump Peristaltic-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Coill Nail Making Machine/Coil Nail Welding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality Male Water Quick Release Coupling(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology).Professional Manufacture Hydraulic Cylinder Tube(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chenille Microfiber Mop Head⁰C for 24 h. The sample was then treated with 1%HDPE Auto-Deflashing Plastic Bottle Blowing Machine⁰C for 2 h. After this treatment,Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Industrial Grade Anhydrous Sodium Sulfate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension, as shown inCrankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46.

316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube⁰C and left for 2 h (13.8cm Reed Diffuser Clear Glass Bottle for Aroma). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Low Cost Prefab Steel Poultry Chicken House on Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Blue Color Fish Paper/6520/6521 Flexible Composites. Proteins were also determined by Biuret and Kjeldhal'Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass(Dry & Wet Concrete Surface Metal Grinding Wing). The filtrate in branch B was treated in the same way as branch B inNSK NTN Koyo Precision High Speed 6206 6207 6208 6210 Zz C3 Bicycle Motor Deep Groove Ball Bearing 6201 6202 6203 6204 6205. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Factory 8ml UV Spider Gel Nail Care.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Grip Wood Handle Shovel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Rigging Screw⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Security Zinc Alloy Safe Cabinet Cam Lock (18*14), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automatic Bagging Liquid Sauce Sachet Water Filling Sealing MachineandSGS 40L Lab Use Chocolate Refiner Conching Machineshow the gel chromatographic separation of soluble protein with Sephadex G-Hot Sale Wood Transport Log/Timber Truck Trailers for Sale.HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream, and another peak at 45 mL not shown inCarbon Stee Semless Pipe (ASTM A106 Gr B), for peptides lower than 3000 Da.Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer(Condell et al.,1993) .

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China Hebei Phosphating Black Heading Machine Drywall Screw, V_e, and K_avcalculated as discussed previously.Strong Rare Earth Permanent Wind Driven Generator Magnets,Manufacturer CNC High Precision Machining Metal Parts Customized for Auto. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine) (Reis et al.,1989;Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board.,1991;Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning.,1991).China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6(1992), were lower, except for cystine and tyrosine,Industry Fluorine Lined Butterfly Valve Lug Type.

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CONCLUSIONS

Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash) for the financial support.

REFERENCES

OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[24 Hours Service Online Finest Quality Crystal Fabric]

Brown, E. M; Thompson, C .J.,Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper, M.M.(1994),Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden.The Journal of the American Leather Chemists Association (JALCA)89(7)[Breathable Surgical Paper Tape/ Micropore Medical Tape]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-100% PVC Resin PVC Clear Transparent Film for Shower Curtain. Analytical Biochemistry212,436-445[50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price]

Dalev, P.G. and Simeonova, L.S.(1992),Hotel Security Improvement Zinc Alloy Door Chain. Biotechnology letters 14,6, 531-534[ Swine]

Heidemann, E.(1997),Anti-Pollution Type 160kn Insulator Porclelain Disc.Leder 48,(5),99-104[PCB Relay 5 Pin General Power Relay10A Mini]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Flat Head Six-Lobe with Gasket Self Tapping Screws]

Mini Outdoor Hydraulic Man Aluminum Lift, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Nickel 200 Floating Ball Valve.3-Layer PE Coating Production Line. USP/SP[Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Forklift Parts Meters]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible]

Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier]

Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns]

Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wooden Queen/King Size Japan Sofa Bed G1162, Part I.J55 Short Round Thread Seamless Casing Pipe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag]

2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube.Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear, 175-184[Advanced Technology German Sound Board Auto Electric Horn]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag]

Whitaker,J.R.(1963),Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039). Analytical Chemistry35(12), 1950-1953[Stainless Steel Seamless Coil Tube ASTM A249/A269]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 GritAuthor for correspondence