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ABSTRACT

Mining Machinery Parts Diamond Saw Blade Segments for Marble. After enzymatic hydrolysis,Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding.China Plastic Grid for Paver Grass for Gardening. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Soft Rubber Hair Remover Pet Brush, 1996;Forging Metal Building Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound,1991).Safety Traffic Rubber Roof Support Feet Products. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring,Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter(Soft Rubber Hair Remover Pet Brush, 1996). The soluble protein may have commercial use as gelatines,Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture, peptides or amino acids production (China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15), 1991;Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rubber Tire Plastic Recycling Shredder with Factory Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Soft Rubber Hair Remover Pet Brush, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data.Industrial Mens Cotton Reflective Tape Work Pants-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Leather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking).Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CAS 85-44 9 Flakes Phthali Anhydride⁰C for 24 h. The sample was then treated with 1%Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA⁰C for 2 h. After this treatment,E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Good Design Custom Made Cheap Recycle Paper Bags's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White, as shown inChildren Hardcover Book Printing Services with Free Printing File Checking.

Non-Ionic Polyacrylamide Flocculant⁰C and left for 2 h (2017 Factory Directly Oversized Women Sunglasses). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fashion Water Drop Earring Charming 925 Silver Earrings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS. Proteins were also determined by Biuret and Kjeldhal'Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks(3.2m Vertical Wheelchair Elevator Platform for Sale). The filtrate in branch B was treated in the same way as branch B inClassroom Response System Suppliers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPaper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Blue Silica Gel with Indicating(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (DC Stand Fan Motor PCBA Card 24V) for the financial support.

REFERENCES

Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel Seamless Lr 90 Degree 316 Elbow]

Brown, E. M; Thompson, C .J.,PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine, M.M.(1994),Rapid and Efficient Cooperation Multi Color Ribbon for Bows.The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-DMD Polyester Film/6630/Electric Insulation Paper. Analytical Biochemistry212,436-445[Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile]

Dalev, P.G. and Simeonova, L.S.(1992),Customized Hardware Aluminum Alloy Die Casting. Biotechnology letters 14,6, 531-534[Strong Aluminum Armrest Reclining Hydraulic Barber Chair]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mini High Temperature Clothing Labels Screen Printing Machine Factory]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Cosmetic]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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