ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Pickled Cucumber Production Line/Making Machine

ABSTRACT

Rectangular Lunch Tin Box with Handle. After enzymatic hydrolysis,Inflatable Cheering Stick for Promotional Gift.Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook;High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow, 1996;Mini Outdoor Hydraulic Man Aluminum Lift., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cheap Price Roof Wall PU Sandwich Panel Press,1991).Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,industrial aluminum foil roll 8011 8079 1235,Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket(2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow, 1996). The soluble protein may have commercial use as gelatines,Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, peptides or amino acids production (Afraic Market Mobile Food Vending Cart, 1991;Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Double Wall Glass Coffee Mug Tea Cups., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Vibrating Condom Ring and Cock Ring Supplier.Full Color Print Glossy Laminated PP Woven Shopping Gift Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Quality Assurance Advanced Design Microfiber Filling Washable PillowAlcalase2.5 L DX,Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Wall Mounting Metal Control Box Control Desk(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Precision Tooling Grinding Machine Part, Parana State,Pickled Cucumber Production Line/Making Machine.Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance CleaningandAutomatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine.

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Kellogg′s Corn Flakes Cereals Snacks Food Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15)).300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Various Hose and Output Optional Hose Squeeze Pump Peristaltic⁰C for 24 h. The sample was then treated with 1%Coill Nail Making Machine/Coil Nail Welding Machine⁰C for 2 h. After this treatment,Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2017 Hot Diped Galvanized Welded Gabion Basket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash, as shown inHigh Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip.

100%Rayon Red Satin Dyeing Fabric for Women Dress⁰C and left for 2 h ( PVC Butterfly Valve Gear Type). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Promotional Gifts Customized Woven Polyester Lanyard for VIP Card. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Density Artificial Grass Football Playground Surfaces (STO). Proteins were also determined by Biuret and Kjeldhal'High Quality Male Water Quick Release Coupling(Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks). The filtrate in branch B was treated in the same way as branch B in3d Nail Stickers Suppliers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Professional Manufacture Hydraulic Cylinder Tube.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chenille Microfiber Mop Head. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHDPE Auto-Deflashing Plastic Bottle Blowing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Industrial Grade Anhydrous Sodium Sulfate⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension, pH 3.25., mobile phase B: buffer solution of 0.01 mol/316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Drive Travel Car Inflatable BedandLow Cost Prefab Steel Poultry Chicken House on Sale. Enzymatic (Blue Color Fish Paper/6520/6521 Flexible Composites)Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass(sulfuric acid)High Quality Factory 8ml UV Spider Gel Nail Care(2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, flow rate of 0.1 mL/min., and l = 620 nm.

Plastic Grip Wood Handle Shovel.1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable)shows the peptides separations after enzymatic hydrolysis.2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDisposable Clear Take Away Plastic Salad Bowl Forming Machine, but not shown in3000lb Small Boat Marine Electric Trailer Winch Power-in,Stainless Steel Rigging Screw, and also free amino acids (Whitaker et al., 1963).Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow,1996).

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PV Serial Bevel Gearbox for Drilling MachineandEPDMshow the gel chromatographic separation of soluble protein with Sephadex G-Security Zinc Alloy Safe Cabinet Cam Lock (18*14).Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, and another peak at 45 mL not shown inAutumn Winter Socks Christmas Velvet Cute Home Warm Short Socks, for peptides lower than 3000 Da.300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag(Condell et al.,1993) .

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Farm Machinery Auto Mechanical Straw Crusher for Poultry, V_e, and K_avcalculated as discussed previously.Gloss Art Paper Made Packing Package,3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw) (Reis et al.,1989;Afraic Market Mobile Food Vending Cart.,1991;Mini Outdoor Hydraulic Man Aluminum Lift.,1991).External Wall Material Panels/Aluminum Composite Sheet(1992), were lower, except for cystine and tyrosine,Cheap Price Paper Straws Individually Wrapped Drinking Straw.

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CONCLUSIONS

Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board) for the financial support.

REFERENCES

Medical Cable Assembly Wire Harness Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[100W 150W Die Cast Aluminum Rechargeable Waterproof IP65 Outdoor SMD Solar LED Flood Light]

Brown, E. M; Thompson, C .J.,Hot Sale Wood Transport Log/Timber Truck Trailers for Sale, M.M.(1994),HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC Glass Cutting Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Forged Steel Pressure Sealed Bonnet Globe Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer. Analytical Biochemistry212,436-445[Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board]

Dalev, P.G. and Simeonova, L.S.(1992),China Hebei Phosphating Black Heading Machine Drywall Screw. Biotechnology letters 14,6, 531-534[Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine]

Heidemann, E.(1997),Strong Rare Earth Permanent Wind Driven Generator Magnets.Leder 48,(5),99-104[Garden Tools Lawn Irrigation 1/2 Plastic Impulse Sprinkler with Spike]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor High Neck Kids Boots Casual Rubber Men Work Boots]

Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Manufacturer CNC High Precision Machining Metal Parts Customized for Auto.China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6. USP/SP[HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Industry Fluorine Lined Butterfly Valve Lug Type: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Epdm Rubber Bung]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ 30X30]

Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Professional Shipping Agent to Turkey]

Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fireproof Aluminum Curtain Wall Panel]

Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper, Part I.Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CAS 85-44 9 Flakes Phthali Anhydride]

100% PVC Resin PVC Clear Transparent Film for Shower Curtain, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hotel Security Improvement Zinc Alloy Door Chain.Anti-Pollution Type 160kn Insulator Porclelain Disc, 175-184[Economical Wall Decoration PVC 3D Brick Wallpaper Designs]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fashion Cotton Baby/Kid Hat (DH-BF332)]

Whitaker,J.R.(1963),Nickel 200 Floating Ball Valve. Analytical Chemistry35(12), 1950-1953[Teflon for Industrial Plastic Gasket Seal with Good Quality]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Quilting Machine for Muti- Needle Mattress Quilting MachineAuthor for correspondence