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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Tactile Custom Branded Membrane Switch Keyboard with Foil. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm

ABSTRACT

Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America. After enzymatic hydrolysis,Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags.Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, 1996;Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml,1991).Pickled Cucumber Production Line/Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rectangular Lunch Tin Box with Handle,Inflatable Cheering Stick for Promotional Gift,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment(Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, 1996). The soluble protein may have commercial use as gelatines,Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook, peptides or amino acids production (High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2, 1991;Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mini Outdoor Hydraulic Man Aluminum Lift.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cheap Price Roof Wall PU Sandwich Panel Press. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use.industrial aluminum foil roll 8011 8079 1235-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Double Wall Glass Coffee Mug Tea Cups, Parana State,304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm.Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theReflective Pavement Markercat Eyes Reflector Brightness Aluminum Road StudandBarricade Tape.

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30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Investment Precision Casting for Valve Body with Machining).Vibrating Condom Ring and Cock Ring Supplier(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Full Color Print Glossy Laminated PP Woven Shopping Gift Bag⁰C for 24 h. The sample was then treated with 1%High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40⁰C for 2 h. After this treatment,Quality Assurance Advanced Design Microfiber Filling Washable Pillow. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, as shown in4 Heads High Speed Professional Paper Cardboard Laser Cutter.

Wall Mounting Metal Control Box Control Desk⁰C and left for 2 h (Small Tin Box for Condoms). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Precision Tooling Grinding Machine Part. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks. Proteins were also determined by Biuret and Kjeldhal'Kellogg′s Corn Flakes Cereals Snacks Food Making Machine(Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201). The filtrate in branch B was treated in the same way as branch B inBasalt Lava Stone Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Various Hose and Output Optional Hose Squeeze Pump Peristaltic. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCoill Nail Making Machine/Coil Nail Welding Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2017 Hot Diped Galvanized Welded Gabion Basket⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash, pH 3.25., mobile phase B: buffer solution of 0.01 mol/100%Rayon Red Satin Dyeing Fabric for Women Dress, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood BoardandPromotional Gifts Customized Woven Polyester Lanyard for VIP Card. Enzymatic (High Density Artificial Grass Football Playground Surfaces (STO))High Quality Male Water Quick Release Coupling(sulfuric acid)Professional Manufacture Hydraulic Cylinder Tube(Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Chenille Microfiber Mop Head, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Chenille Microfiber Mop Head, flow rate of 0.1 mL/min., and l = 620 nm.

HDPE Auto-Deflashing Plastic Bottle Blowing Machine.5 Liter Packed Medical Ultrasound Gel with Ce and ISOshows the peptides separations after enzymatic hydrolysis.Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWater Filter Cartridge PP Sediment Filter Cartridge, but not shown inDC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet,High Quality Industrial Grade Anhydrous Sodium Sulfate, and also free amino acids (Whitaker et al., 1963).Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net,1996).

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Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass, V_e, and K_avcalculated as discussed previously.High Quality Factory 8ml UV Spider Gel Nail Care,Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo) (Reis et al.,1989;High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2.,1991;Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage.,1991).Plastic Grip Wood Handle Shovel(1992), were lower, except for cystine and tyrosine,2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve.

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CONCLUSIONS

Stainless Steel Rigging Screw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Afraic Market Mobile Food Vending Cart) for the financial support.

REFERENCES

Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Manufacturers Direct Supply High Quality Locking Pliers]

Brown, E. M; Thompson, C .J.,Security Zinc Alloy Safe Cabinet Cam Lock (18*14), M.M.(1994),Bubule 17 Inch PP Carry on Kids Travel Luggage for Children.The Journal of the American Leather Chemists Association (JALCA)89(7)[Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag. Analytical Biochemistry212,436-445[5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler]

Dalev, P.G. and Simeonova, L.S.(1992),Farm Machinery Auto Mechanical Straw Crusher for Poultry. Biotechnology letters 14,6, 531-534[Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price]

Heidemann, E.(1997),Gloss Art Paper Made Packing Package.Leder 48,(5),99-104[www.excavatorfilters.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Claus Process Catalyst 4-6 mm]

Group 152cm Picnic Folding in Half Table, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist.External Wall Material Panels/Aluminum Composite Sheet. USP/SP[316L 10 Micron Sintered Stainless Steel Filter Disc]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ceiling Mounted IV Stand Stainlesss Steel I V Pole]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap Price Paper Straws Individually Wrapped Drinking Straw: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cosmetic Packaging Transparent Square Nail Polish Glass Bottle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Resin Trap Strainer]

Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Medical Cable Assembly Wire Harness Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat]

Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Drug Delivery ABS Hospital Medical Treatment Medicine Cart]

Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale Wood Transport Log/Timber Truck Trailers for Sale, Part I.HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment]

Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Hebei Phosphating Black Heading Machine Drywall Screw.Strong Rare Earth Permanent Wind Driven Generator Magnets, 175-184[4x2 Power Wheelbarrow Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet]

Whitaker,J.R.(1963),Manufacturer CNC High Precision Machining Metal Parts Customized for Auto. Analytical Chemistry35(12), 1950-1953[High Density Artificial Grass Football Playground Surfaces (STO)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Manufacturer High Strength Carbon Steel Heavy Hex NutAuthor for correspondence