ARTICLES

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ABSTRACT

Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2). After enzymatic hydrolysis,Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture.Cute Cartoon Coin Purse Children Zipper Purse. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tamper Evident Security Seals Heat Resistant Electronic Tape, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (South Africa Standards Solar Power Variable Message Sign LED Traffic Sign, 1996;2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels,1991).Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mooncake Pastry and Plastic Packaging Box,Complete Maize Starch Wet Milling Plant with High Quality,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole(South Africa Standards Solar Power Variable Message Sign LED Traffic Sign, 1996). The soluble protein may have commercial use as gelatines,Top Furniture Cheap Iron Stacking Banquet Hall Chairs, peptides or amino acids production (Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle, 1991;Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Document Storage Durable Metal Wire Mesh Decking for Pallet Rack. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (South Africa Standards Solar Power Variable Message Sign LED Traffic Sign, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mosquito Proof Fiberglass Fly Screen.Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cooling Tower Replaced Fill Media, Parana State,Geotextile for Airport Runways/High Way, Railway.A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (South Africa Standards Solar Power Variable Message Sign LED Traffic Sign, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGas Calcined Anthracite Carburizing Agentand5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure.

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Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Crane Hook Qtz250 (7032) with 70m Jib Length).Stainless Steel Expanded Mesh(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2⁰C for 24 h. The sample was then treated with 1%Lf + Hf Contactless Smart Hybrid Card⁰C for 2 h. After this treatment,Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LED Fairy Star Light for All Kinds of Decoration Skylike's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply, as shown in120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay.

Linear Vibrator Screener, for Granule and Powder Selection and Classification⁰C and left for 2 h (Men Casual Short Pants Custom Quality Running Sports Wear Shorts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Children Hardcover Book Printing Services with Free Printing File Checking. Proteins were also determined by Biuret and Kjeldhal'One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid(Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course). The filtrate in branch B was treated in the same way as branch B inP Trap Factory & Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Global Brands 10 Year Cheaper Hook & Loop Tape Male Side.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Large Size Stone Aluminum Honeycomb Panel for Facade, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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12oz 16oz Glass Mason Jar with Handle and Straw.12/24V 150ah Power Battery Chargershows the peptides separations after enzymatic hydrolysis.Mini Freeze Dryer for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inV Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine, but not shown inWomen Summer Swimsuit Chest Zip Bathing Suit,5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber, and also free amino acids (Whitaker et al., 1963).Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (South Africa Standards Solar Power Variable Message Sign LED Traffic Sign,1996).

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Flat Head Six-Lobe with Gasket Self Tapping Screws, V_e, and K_avcalculated as discussed previously.DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw,Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg) (Reis et al.,1989;Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle.,1991;2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin.,1991).0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting(1992), were lower, except for cystine and tyrosine,Weiying Full Computerized Plain and Invisible Sock knitting Machine.

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CONCLUSIONS

Hot Double Cereal Airtight Food Dispenser. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Replica Car Wheels with 20inch for BMW Car) for the financial support.

REFERENCES

Disposable Food Packaging Paper Soup Bowl with Lids, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Men′s Travel Bag Trolley Luggage Duffle Bag Oxford Travel Luggage]

Brown, E. M; Thompson, C .J.,500ml PP Sport Water Bottle with Ball, M.M.(1994),Stamping Fine Blanking Sheet Metal Fabrication Part.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins. Analytical Biochemistry212,436-445[Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker]

Dalev, P.G. and Simeonova, L.S.(1992),Trade Assurance Beautiful Silk Lace Closure. Biotechnology letters 14,6, 531-534[LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W]

Heidemann, E.(1997),High Quality Needle Rollers for Bearings.Leder 48,(5),99-104[Customized Inflatable Paradise Bouncers Toy (AQ1370)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2-100mm Waterproof High Density Polyethylene PE HDPE Plastic Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty]

High Quality Bulk Price Yk-11 Powder for Bodybuilding, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Trade Assurance Beautiful Silk Lace Closure]

High Quality Bulk Price Yk-11 Powder for Bodybuilding,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings]

High Quality Bulk Price Yk-11 Powder for Bodybuilding, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer, Part I.Meet All Industry Needs with Openly Design High Temperature Oven. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2019 Kiet Hydraulic Pipe Bender Machine]

Whitaker,J.R.(1963),2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery. Analytical Chemistry35(12), 1950-1953[Disposable Sugarcane Pulp Paper Bowl 500ml]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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