ARTICLES

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ABSTRACT

DC Colorful Battery Operated Small Hand Fan for Outdoor Use. After enzymatic hydrolysis,Hsc Heavy Duty 5 Ton Chain Pulley Block.Outdoor Christmas Decoration Highway Street Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.48V 200ah UPS Battery Backup for Telecom, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray, 1996;Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment,1991).Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,8L Mini Fridge Freezer for Home and Car Fridge,Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,23m 4 R Arms 2017 New Design Concrete Placing Boom(450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray, 1996). The soluble protein may have commercial use as gelatines,Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle, peptides or amino acids production (China Factory Offer New Core I7 15.6 Laptop, 1991;Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mining Machinery Parts Diamond Saw Blade Segments for Marble., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Plastic Grid for Paver Grass for Gardening. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Explosion Proof Rechargeable LED Searchlight.Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter).Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15)⁰C for 24 h. The sample was then treated with 1%Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor⁰C for 2 h. After this treatment,Rubber Tire Plastic Recycling Shredder with Factory Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile, as shown inWholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood.

Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data⁰C and left for 2 h (Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Industrial Mens Cotton Reflective Tape Work Pants. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye. Proteins were also determined by Biuret and Kjeldhal'Aluminum Coil with AA3003 AA3105 H14 H16 H18(Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering). The filtrate in branch B was treated in the same way as branch B inDouble Screws Textured Soy Protein Artificial Meat Tvp Tsp Extruder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DC Stand Fan Motor PCBA Card 24V. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEconomic Brwon Kraft Slip Sheet Tray and Pallet Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Power Tools Lithium Battery Cordless Drill (GBK2-66108TS), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Non-Ionic Polyacrylamide Flocculant.Stainless Steel 304 Wire Rope Fork Terminalshows the peptides separations after enzymatic hydrolysis.Fashion Water Drop Earring Charming 925 Silver Earrings(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2, but not shown inMini Outdoor Hydraulic Man Aluminum Lift,Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS, and also free amino acids (Whitaker et al., 1963).Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray,1996).

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Blue Silica Gel with Indicating, V_e, and K_avcalculated as discussed previously.Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system,Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Dyed Colored Sand) (Reis et al.,1989;China Factory Offer New Core I7 15.6 Laptop.,1991;Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks.,1991).Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)(1992), were lower, except for cystine and tyrosine,Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board.

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CONCLUSIONS

High Precision Green Sand Casting Ball Valve Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound) for the financial support.

REFERENCES

Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[74% Calcium Chloride High Quality Supplier]

Brown, E. M; Thompson, C .J.,Class 300 1/2 Stainless Steel Forging Type Check Valve, M.M.(1994),CAD Cam Dental Machine 3D Dental Model Printer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automotive OEM Copper Wire Harness and Connectors for Industry]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Low Volume Production Aluminum CNC Machining Auto Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-900kg Pneumatic Motorcycle Lift. Analytical Biochemistry212,436-445[Seashell Special Shape Magnetic Eye Shadow Empty Palette]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Power Extension Cord AC Cable Plug for Office Equipment. Biotechnology letters 14,6, 531-534[300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Zinc Plating White DIN 934 Hexagon Nut]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler]

Etched Stainless Steel Titanium Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes]

Etched Stainless Steel Titanium Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[DMD Polyester Film/6630/Electric Insulation Paper]

Etched Stainless Steel Titanium Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, Part I.Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Small Poultry Pig Feed Farm Steel Silo for Sale]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board]

Whitaker,J.R.(1963),Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6. Analytical Chemistry35(12), 1950-1953[1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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