ARTICLES

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ABSTRACT

3D Good Quality Laser Engraving Machine for Souvenirs Shop. After enzymatic hydrolysis,Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories.3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Reverse Flow Configuration Axial Fan;Handcraft Wood Jewelry Storage Box with Lock and Key; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Single Spindle Micro CNC Lathe Ck0640, 1996;Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pipe Thermostat with Sensor Ce Certificate,1991).High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring,Nitinol Disposable Guide Wire,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Breathable Surgical Paper Tape/ Micropore Medical Tape(Single Spindle Micro CNC Lathe Ck0640, 1996). The soluble protein may have commercial use as gelatines,Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow, peptides or amino acids production (Crane Hook Qtz250 (7032) with 70m Jib Length, 1991;China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Online High Sensitivity Checkweigher with Pusher Bar. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Single Spindle Micro CNC Lathe Ck0640, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Piggery Farm Pig/Sow Gestation Equipment From China Factory.Lw250*700 Spiral Discharge Decanter Separator for Water Treatment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Single Cheap Custom Festival Woven RFID Fabric Wristband for Events(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Thickness EVA High Density Foam EVA Roll).Outdoor High Neck Kids Boots Casual Rubber Men Work Boots(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)⁰C for 24 h. The sample was then treated with 1%Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector⁰C for 2 h. After this treatment,2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Grinding Machining Parts High Precision's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, as shown inFast Delivery Malleable Iron Pipe Fitting Hexagon Nipple.

Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag⁰C and left for 2 h (Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Disposable Clear Take Away Plastic Salad Bowl Forming Machine. Proteins were also determined by Biuret and Kjeldhal'Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control(China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker). The filtrate in branch B was treated in the same way as branch B inChain Link Fence Top Barbed Wire. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCarbon Stee Semless Pipe (ASTM A106 Gr B)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Selling House Floor Cleaning 360 Spin Mop, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Roots VacuumandAdvanced Unlocking Tool for Locksmith. Enzymatic (High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts)Fashion Design Sleeveless Fitness Wear(sulfuric acid)DC Colorful Battery Operated Small Hand Fan for Outdoor Use(Single Spindle Micro CNC Lathe Ck0640, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hsc Heavy Duty 5 Ton Chain Pulley Block, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hsc Heavy Duty 5 Ton Chain Pulley Block, flow rate of 0.1 mL/min., and l = 620 nm.

Outdoor Christmas Decoration Highway Street Light.Wire Mesh Welding Size 10mm X 10mmshows the peptides separations after enzymatic hydrolysis.Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPromotional Gifts Customized Woven Polyester Lanyard for VIP Card, but not shown inPA Flexible Plastic Corrugated Loom Tubing,China Cheap Newest 70*77cm XPE Foam 3D Wallpaper, and also free amino acids (Whitaker et al., 1963).48V 200ah UPS Battery Backup for Telecom. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Single Spindle Micro CNC Lathe Ck0640,1996).

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AISI 430 Ba/2b Embossed Stainless Steel SheetandAbstract Light and Shadow Metal Oil Painting for Decoshow the gel chromatographic separation of soluble protein with Sephadex G-450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray.Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks, and another peak at 45 mL not shown inCeramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, for peptides lower than 3000 Da.5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment(Condell et al.,1993) .

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CONCLUSIONS

China Factory Offer New Core I7 15.6 Laptop. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass) for the financial support.

REFERENCES

Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.gsrfid.com]

Brown, E. M; Thompson, C .J.,Mining Machinery Parts Diamond Saw Blade Segments for Marble, M.M.(1994),Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding.The Journal of the American Leather Chemists Association (JALCA)89(7)[Carbon Steel Spherical Plain Radical Bearings]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Carbon Steel 20g Gr. B Steam Cooling Fin Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Plastic Grid for Paver Grass for Gardening. Analytical Biochemistry212,436-445[China Factory Price Hg1 Self Adjustable Pipe Tank Rotator]

Dalev, P.G. and Simeonova, L.S.(1992),Explosion Proof Rechargeable LED Searchlight. Biotechnology letters 14,6, 531-534[Puer/off White Melamine Particle Board/MDF Board]

Heidemann, E.(1997),Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1.Leder 48,(5),99-104[Best Fuel Filter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA]

Wheel Loader Transmission Gear Box/Customized Casting Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair.Soft Rubber Hair Remover Pet Brush. USP/SP[Pickled Cucumber Production Line/Making Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Forging Metal Building Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Factory 8ml UV Spider Gel Nail Care]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Marine Floating 2.5 Inch Oil Rubber Pipe for Fuel Delivery]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Home Appliance Blender Juicer Motor]

Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Safety Traffic Rubber Roof Support Feet Products-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic]

Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hotel Security Improvement Zinc Alloy Door Chain]

Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring, Part I.Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[33kv 100kVA Dry-Type Distribution Transformer]

Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture.China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15), 175-184[304 316 316L Mirror 8K Golden Stainless Steel Sheet 1000X2000mm 1219mmx2438mm 1220mmx2440mm]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ GRP Grating]

Whitaker,J.R.(1963),Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor. Analytical Chemistry35(12), 1950-1953[Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Zinc Coated Galvanized WireAuthor for correspondence