ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-DMD Polyester Film/6630/Electric Insulation Paper. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Customized Hardware Aluminum Alloy Die Casting

ABSTRACT

Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles. After enzymatic hydrolysis,SGS 40L Lab Use Chocolate Refiner Conching Machine.Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve, 1996;600mm Warehouse No Power Roof Fan., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Small Brown Glass Bottles,1991).Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe,RF China Professional Manufacturer Direct Way Manual Ball Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected(5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve, 1996). The soluble protein may have commercial use as gelatines,Stainless Braided PTFE Hose R14 Standard Pipe, peptides or amino acids production (SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser, 1991;Black Steel ASTM A192 Seamless Pipe Sch120, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Raw Material Adhesive NBR Latex of Latex Long Gloves., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress.1200X1000X810 Strong Collapsible Plastic Containers for Storage-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fast Install Polyurethane Decorative Wall Panel for Homes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Tensisle Strength Tissue Paper, Parana State,Customized Hardware Aluminum Alloy Die Casting.High Zinc Coated Galvanized Wire, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mmand10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine.

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Women Summer Swimsuit Chest Zip Bathing Suit(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Gold Inlaid 24K Gold / Flowers).Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Female Header Conn Ptich 1.0mm Connector Btb⁰C for 24 h. The sample was then treated with 1%Tie Down Nylon Back to Back Hook and Loop Straps⁰C for 2 h. After this treatment,Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks, as shown inDouble Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine.

Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine⁰C and left for 2 h (CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Warm Paste Packaging Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Resin Trap Strainer. Proteins were also determined by Biuret and Kjeldhal'Small Bar Fridge with Glass Door for Beverage and Beer(18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring). The filtrate in branch B was treated in the same way as branch B inBraider. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheap Loader Xt860 Backhoe Loader with Digger. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Lithium Battery 20ah Pouch 3c Cell 3.7V⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Handweel Three-Way Non-Retention Ball Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery.Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farmshows the peptides separations after enzymatic hydrolysis.CNC Glass Cutting Line(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAfraic Market Mobile Food Vending Cart, but not shown inOil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe,Small & Tiny CNC Precision Machining Brass Parts Metal Part, and also free amino acids (Whitaker et al., 1963).Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve,1996).

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CONCLUSIONS

Cleaning Lint Free Microfiber Mop. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, M.M.(1994),LED Emergency Exit Light Rechargeable 220V.The Journal of the American Leather Chemists Association (JALCA)89(7)[China water meter factory water flow meter vane wheel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector. Analytical Biochemistry212,436-445[ School Furniture (GT-76C)]

Dalev, P.G. and Simeonova, L.S.(1992),Firefighting Equipment Aluminum Rescue Tripod with Winch 30m. Biotechnology letters 14,6, 531-534[OEM Service Machining Milling Turning Service Custom CNC Aluminum Case]

Heidemann, E.(1997),Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279.Leder 48,(5),99-104[Jhg Silica Sand Weight]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cjx2-F (LC1-F) AC Magnetic Contactor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3D Good Quality Laser Engraving Machine for Souvenirs Shop]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Silicone Bead]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200]

30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cleaning Lint Free Microfiber Mop]

30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Factory Queen Mattress with Visco Memory Foam and Latex]

30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan, Part I.Aluminum/Aluminium Extrusion Profile Housing for LED Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors]

Whitaker,J.R.(1963),Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine. Analytical Chemistry35(12), 1950-1953[220V/50Hz Stainless Steel Ozone Generator with 5g/H]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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