ARTICLES

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ABSTRACT

Cooling Tower Replaced Fill Media. After enzymatic hydrolysis,A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow.Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Expanded Mesh;Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lf + Hf Contactless Smart Hybrid Card, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses, 1996;LED Fairy Star Light for All Kinds of Decoration Skylike., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply,1991).Linear Vibrator Screener, for Granule and Powder Selection and Classification. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket,Children Hardcover Book Printing Services with Free Printing File Checking,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid(Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses, 1996). The soluble protein may have commercial use as gelatines,Global Brands 10 Year Cheaper Hook & Loop Tape Male Side, peptides or amino acids production (New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw, 1991;Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Large Size Stone Aluminum Honeycomb Panel for Facade. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System.Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Making Machine for EVA Soft Hose(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Mini Freeze Dryer for Sale, Parana State,SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing.5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAnti-Pollution Type 160kn Insulator Porclelain DiscandA19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb.

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Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory).CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality and Efficient Combustion UHP Graphite Electrode⁰C for 24 h. The sample was then treated with 1%Graphite Ring Good Lubrication⁰C for 2 h. After this treatment,Flat Head Six-Lobe with Gasket Self Tapping Screws. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile, as shown in10in 1500lb Pressure Seal Forged Steel Swing Check Valve.

0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting⁰C and left for 2 h (Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Weiying Full Computerized Plain and Invisible Sock knitting Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Double Cereal Airtight Food Dispenser. Proteins were also determined by Biuret and Kjeldhal'Disposable Food Packaging Paper Soup Bowl with Lids(Professional Manufacture Hydraulic Cylinder Tube). The filtrate in branch B was treated in the same way as branch B inSteering Rack For Toyota Corona. The final soluble protein shown were used for peptides separations using gel permeation chromatography,500ml PP Sport Water Bottle with Ball.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stamping Fine Blanking Sheet Metal Fabrication Part. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFng Circular Push Pull Male Connectors with Lanyard 0b 6pins(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Needle Rollers for Bearings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heavy Duty Steel Warehouse Storage Pallet Rack, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Best Price Plastic Spc Click PVC Vinyl Flooring for 5mmandHigh Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire. Enzymatic (High Quality Bulk Price Yk-11 Powder for Bodybuilding)Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED(sulfuric acid)PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer(Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Meet All Industry Needs with Openly Design High Temperature Oven, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Meet All Industry Needs with Openly Design High Temperature Oven, flow rate of 0.1 mL/min., and l = 620 nm.

380V 3.0HP Peristaltic Pump Swimming Pool Pump.Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)shows the peptides separations after enzymatic hydrolysis.Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBest Selling Wooden Grain Metal Aluminium Fence Panels for Sale, but not shown inCS1363 a Standard UK 3 Pin Power Plug,14 Gauge Twist Galvanized Black Annealed Steel Wire, and also free amino acids (Whitaker et al., 1963).2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses,1996).

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Full-Automatic Folding Napkin Paper Machine, V_e, and K_avcalculated as discussed previously.Customized Frame Mount Highway Pegs Enclosure Parts,Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine) (Reis et al.,1989;New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw.,1991;LED Fairy Star Light for All Kinds of Decoration Skylike.,1991).10mm Tempered Bathroom Glass Safety Glass for Building(1992), were lower, except for cystine and tyrosine,3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet.

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CONCLUSIONS

Cattle Feed Factory Used Dust Collector and Filter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top) for the financial support.

REFERENCES

Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Milling Machining Parts]

Brown, E. M; Thompson, C .J.,Group 152cm Picnic Folding in Half Table, M.M.(1994),Tactile Custom Branded Membrane Switch Keyboard with Foil.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Solid Carbide Single Flute End Mills Oblique Angle Cutting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm. Analytical Biochemistry212,436-445[OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape]

Dalev, P.G. and Simeonova, L.S.(1992),Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America. Biotechnology letters 14,6, 531-534[2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine]

Heidemann, E.(1997),Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags.Leder 48,(5),99-104[Cellulose Professional Manufacturer Supplier & Factory]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[12V Car Cigarette Lighter Adapter Power Extension Cord]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PE100 Plasitc Pipe for Gas Water Chemcial Sewage System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Selling Round Cookware Pan with Induction Bottom Frying Pan]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ 36V]

Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Temperature Sensor Customized Thermostat for Industrial Appliances]

Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood]

Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage, Part I.Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Promotion Indian Market Underwear Adhesive Glue]

Pickled Cucumber Production Line/Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rectangular Lunch Tin Box with Handle.Inflatable Cheering Stick for Promotional Gift, 175-184[Custom Full Color Printing Overlocking Edge Gaming Mouse Pad]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube]

Whitaker,J.R.(1963),Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment. Analytical Chemistry35(12), 1950-1953[1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wind Protection Screen. Wind Dust NettingAuthor for correspondence