ARTICLES

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ABSTRACT

9mm/12mm Seven Color Exposed Luminous Pixel String LED. After enzymatic hydrolysis,Construction Scaffolding Steel Galvanized Acrow Prop.3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8;Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.AKD Wax 1895/Alkyl Ketene Dimer 1895, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (22.8V 150W Medical Halogen Lamp, 1996;Aluminum Conductor PE Insulated Triplex Cable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Home Appliance Blender Juicer Motor,1991).Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices,13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price(22.8V 150W Medical Halogen Lamp, 1996). The soluble protein may have commercial use as gelatines,Tractor Tine Tiller Cultivator, peptides or amino acids production (Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins, 1991;CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LSR Injection Mold Tooling for Medical Nipple.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Nail Stamping Plates Custom Metal Part Sheet Metal Stamping. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (22.8V 150W Medical Halogen Lamp, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Sports Jacket Men Outdoor Waterproof Jackets.Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Smart Big Hydroponic Indoor Home Garden Planter, Parana State,Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t.China Quality Heavy Duty Globe Control Valve, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (22.8V 150W Medical Halogen Lamp, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID CardandHigh Quality Cheap Price Disposable IV Infusion Set with Precise Filter.

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Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wooden Queen/King Size Japan Sofa Bed G1162).China Suppliers Laser Marking Equipment of Fiber Laser(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Bonfiglioli Ta Series Helical Gearing Gearbox⁰C for 24 h. The sample was then treated with 1%Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+⁰C for 2 h. After this treatment,LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Large Stock Aerators for Shrimp Farms and Fish Pond, as shown inNitinol Disposable Guide Wire.

750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.⁰C and left for 2 h (0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Large Diameter Aluminum Pipe 3004. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory. Proteins were also determined by Biuret and Kjeldhal'OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder(Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price). The filtrate in branch B was treated in the same way as branch B in4.0 Big Tyre Bicycle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Integrated Waterproof PVC Lamination Bathroom Wall Panels.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Embossed PVC Rexine Car Seat Cover Leather. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Grade Aluminum Fly Fishing Thermometer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Forged 316 Stainless Steel Marine Twisted Long D Shackle. Educational Equipment Whiteboardshows the peptides separations after enzymatic hydrolysis.2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGraphite Ring Good Lubrication, but not shown in Agrochemicals,Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels, and also free amino acids (Whitaker et al., 1963).Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (22.8V 150W Medical Halogen Lamp,1996).

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Pressure Resistance Al2O3 Alumina Ceramic Mold Guide PinsandHigh Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCBshow the gel chromatographic separation of soluble protein with Sephadex G-Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04.Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight, and another peak at 45 mL not shown inBiostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer, for peptides lower than 3000 Da.355nm UV Laser Marking Equipment for Crystal Materials(Condell et al.,1993) .

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CONCLUSIONS

Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality) for the financial support.

REFERENCES

High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China ISO Manufacturer Cheap Plastic CNC Machining Rapid Prototype Fabrication Service]

Brown, E. M; Thompson, C .J.,Quilting Machine for Muti- Needle Mattress Quilting Machine, M.M.(1994),Single-Screw Plastic Extruders Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[American NEMA Straight Blade Plug 30A 125V/250V Power Plug]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube. Analytical Biochemistry212,436-445[Advanced Unlocking Tool for Locksmith]

Dalev, P.G. and Simeonova, L.S.(1992),High Quanlity Colorful Aluminum Spacer/Standoff. Biotechnology letters 14,6, 531-534[P4.75 Indoor Single Dual Color Advertising LED Display Billboard]

Heidemann, E.(1997),5g OPP Silica Gel Desiccant.Leder 48,(5),99-104[Off Road Skateboard]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Cheap Newest 70*77cm XPE Foam 3D Wallpaper]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Geotextile for Airport Runways/High Way]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Truck Air Dryer cartridge 14bar: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Backward Spinning SEGMENT]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce]

Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3mm White Waterproof PVC Foam Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Sensitivity Ground Metal Detector Searching for Treasure]

Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Anti-Corrosion Fiberglass Sheet and Roll]

Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Educational Equipment Smart Multi Touch Interactive White Board for School, Part I.Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[E6000 Handheld Three Phase Power Quality and Energy Analyzer]

Women Party Jewelry 45cm Sequins Choker, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity.High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe, 175-184[Swinging Fitting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools]

Whitaker,J.R.(1963),High Freqency PCB with Blind Via. Analytical Chemistry35(12), 1950-1953[Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

2018 New Design Red-CNC-006 CNC Machining Aluminum PrecisionAuthor for correspondence