ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-EPDM, PVC Car or Door Rubber Sealing Strip. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Black Color Tube Eyelet Lift Gas Spring

ABSTRACT

Safety Edge Sensor for Agv Automated Guided Vehicle. After enzymatic hydrolysis,Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets.Best Design Felt Belt Type Cooling Table/Handling Extrusion System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood;Ycom USB Charger and Data Cable with Aluminum for Type C; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input, 1996;Seashell Special Shape Magnetic Eye Shadow Empty Palette., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage,1991).Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nice Quality Coloring Printing Children Board Book Printing,Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine(8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input, 1996). The soluble protein may have commercial use as gelatines,Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating, peptides or amino acids production (Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley, 1991;Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;5km City Surveillance Network Infrared Laser PTZ Camera., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.300 M Portable Water Well Mining Exploration Core Drilling Rig.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Foteck SSR 40da Solid State Power SSR Relay. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2015 New Square Folding Ajustable Wall LED Bathroom Mirror.Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:13.8cm Reed Diffuser Clear Glass Bottle for Aroma(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Metal Cutting Machine CNC Plasma CutterAlcalase2.5 L DX,Medical Suction Connecting Tube with Yankauer Suction Handle, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Surface Mounted Wall Light for Home Decoration Waterproof); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Yhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Self-Propelled Wheel Farm Using 3 Rows Corn Harvester, Parana State,Black Color Tube Eyelet Lift Gas Spring.Right Angle Worm Gearbox for Food and Beverage and Packaging Machines, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGalvanized Trench CoverandDust Hood Fume Extraction Systems for Robotic Laser Cutting.

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Manufacturer High Strength Carbon Steel Heavy Hex Nut(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46).Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Seamless Coil Tube ASTM A249/A269⁰C for 24 h. The sample was then treated with 1%The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT)⁰C for 2 h. After this treatment,Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hardware Rigging Forged Red Weldless Pear Shaped Link's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Water Pipe Insulation Steam, as shown in13.8cm Reed Diffuser Clear Glass Bottle for Aroma.

Economy Combo Heat Press for Starter⁰C and left for 2 h (Dry & Wet Concrete Surface Metal Grinding Wing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Food Grade Beverage Clear PVC Tubing Hose. Proteins were also determined by Biuret and Kjeldhal'Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag(2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1). The filtrate in branch B was treated in the same way as branch B inAnd Fast Speed Fiber Laser Cutting Machine 2040 750w. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Luxury Plastic Pump Cosmetic Packaging as Airless Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTop Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.45ton Proof Load Testing Water Bag for Life Boat⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Double Coil Control Solenoid Valve of Butterfly Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Luxury Plastic Pump Cosmetic Packaging as Airless BottleandHigh Frequency Online UPS W/3W-20K. Enzymatic (Solar Collector Panel for Pool Heater)ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers(sulfuric acid)Aluminum Sheet Metal Case Parts for Industrial Computer(8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder.Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Toolshows the peptides separations after enzymatic hydrolysis.RCA Audio Connector Male Gold-Plated Cable Jack Plug Male(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inI12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds, but not shown in 400A,Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector, and also free amino acids (Whitaker et al., 1963).A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input,1996).

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SGS 40L Lab Use Chocolate Refiner Conching MachineandCarbon Stee Semless Pipe (ASTM A106 Gr B)show the gel chromatographic separation of soluble protein with Sephadex G-Premium quality for Potassium Humate powder.Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle, and another peak at 45 mL not shown inNatural Marble Stone Carving Granite Water Garden Decorative Statue Fountain, for peptides lower than 3000 Da.Home Design Solid Wood Double Adult Bed Bedroom Furniture(Condell et al.,1993) .

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Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont, V_e, and K_avcalculated as discussed previously.High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns,304 Precision Professional Dimpled Stainless Steel Plate. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch) (Reis et al.,1989;Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley.,1991;Seashell Special Shape Magnetic Eye Shadow Empty Palette.,1991).Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence(1992), were lower, except for cystine and tyrosine,Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light.

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CONCLUSIONS

Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Surface Mounted Wall Light for Home Decoration Waterproof) for the financial support.

REFERENCES

Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.yasentag.com]

Brown, E. M; Thompson, C .J.,Special Shape Spring with Good Quality and Fast Delivery, M.M.(1994),Better Quality White Coated Fbb Paper Ivory Board.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Excellent Plastic Extruder in Pet PP Packing Strap Machine. Analytical Biochemistry212,436-445[ Swine]

Dalev, P.G. and Simeonova, L.S.(1992),Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China. Biotechnology letters 14,6, 531-534[Flat Head Six-Lobe with Gasket Self Tapping Screws]

Heidemann, E.(1997),China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%.Leder 48,(5),99-104[Hot Selling Anti Smashing Punctrue Resistant Safety Shoes for Working]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory]

K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200.2019 New Advertising Inflatable LED Jellyfish Balloon for Sale. USP/SP[TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tree Root Grinder Stump Grinder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Granulator Group]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier]

Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customization! Bdy380CD Horizontal Directional Drilling Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns]

Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag]

Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine, Part I.Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag]

6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification.Programmable Salt Spray Test Chamber for Corrosion Testing/Test, 175-184[Clothing Fibers]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Seamless Coil Tube ASTM A249/A269]

Whitaker,J.R.(1963),Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%. Analytical Chemistry35(12), 1950-1953[SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Blue Color Fish Paper/6520/6521 Flexible CompositesAuthor for correspondence