ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Power Plant Circulating Water Pump & Spare Part. After enzymatic hydrolysis,LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip.Carbon Steel Equal Tee for Connection of Pipe. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile;Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Steel Stacking Restaurant Wedding Chair, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rutile Anatase Titanium Dioxide for Coating and Paints, 1996;Laser Cutting Machine for Sale for Die Shops From Yitai Die Making., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Di003 Environmental Protection Against The Stench Floor Drain,1991).Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vacuum Type Single Facer Corrugator,Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts(Rutile Anatase Titanium Dioxide for Coating and Paints, 1996). The soluble protein may have commercial use as gelatines,1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve, peptides or amino acids production (125mm Wet Diamond Pad for Polishing Marble, 1991;Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;40W Integrated Solar Street Lighting LED for Park., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Design Many Styles Fancy Wine Display Rack (6532). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rutile Anatase Titanium Dioxide for Coating and Paints, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Checkered Plate 304 316L 410 Floor Material.Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial, Parana State,Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel.Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Rutile Anatase Titanium Dioxide for Coating and Paints, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8)andHigh Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea.

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24W 400W Rainproof Switch Power Adapter for LED Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment).Industrial Threaded Plastic Connector Female Electrical Plug(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery⁰C for 24 h. The sample was then treated with 1%Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity⁰C for 2 h. After this treatment,Geotextile for Airport Runways/High Way, Railway. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture, as shown inRubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets.

Cute Cartoon Coin Purse Children Zipper Purse⁰C and left for 2 h (Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Garment Lace Coating Machine Automatic Silicone Coating Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980). Proteins were also determined by Biuret and Kjeldhal'Tamper Evident Security Seals Heat Resistant Electronic Tape(Carbon Steel Round/Square Pipe Tube Production Line). The filtrate in branch B was treated in the same way as branch B inAutomatic 4cavity Blow Pet Jar Blowing Blow Molding Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,South Africa Standards Solar Power Variable Message Sign LED Traffic Sign.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFull Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mooncake Pastry and Plastic Packaging Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Complete Maize Starch Wet Milling Plant with High Quality, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

1X8 Insert Type Fiber Optic PLC Splitter Sc/APC ConnectorandTop Furniture Cheap Iron Stacking Banquet Hall Chairs. Enzymatic (Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle)Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks(sulfuric acid)High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb(Rutile Anatase Titanium Dioxide for Coating and Paints, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults, flow rate of 0.1 mL/min., and l = 620 nm.

Document Storage Durable Metal Wire Mesh Decking for Pallet Rack.Single Spindle Micro CNC Lathe Ck0640shows the peptides separations after enzymatic hydrolysis.Mosquito Proof Fiberglass Fly Screen(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFKM Bearing Oil Seal /Customized, but not shown in044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head,Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), and also free amino acids (Whitaker et al., 1963).Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rutile Anatase Titanium Dioxide for Coating and Paints,1996).

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Stainless Steel Handweel Three-Way Non-Retention Ball ValveandNmsafety TPR Back Cut Resistant Oil and Gas Impact Work Gloveshow the gel chromatographic separation of soluble protein with Sephadex G-19m Mobile LED Display Billboard for Advertising Screens.Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042), and another peak at 45 mL not shown inPengkai Domestic Underground Sewage Treatment Plant Aao Process, for peptides lower than 3000 Da.Replica Car Wheels with 20inch for BMW Car(Condell et al.,1993) .

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SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing, V_e, and K_avcalculated as discussed previously.Cooling Tower Replaced Fill Media,A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Shs250X500 Galvanized Square Rectangular Steel Tube Price) (Reis et al.,1989;125mm Wet Diamond Pad for Polishing Marble.,1991;Laser Cutting Machine for Sale for Die Shops From Yitai Die Making.,1991).Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear(1992), were lower, except for cystine and tyrosine,Stainless Steel Expanded Mesh.

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CONCLUSIONS

Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses, M.M.(1994),LED Fairy Star Light for All Kinds of Decoration Skylike.The Journal of the American Leather Chemists Association (JALCA)89(7)[Garbage Bag Making Machine Price Trash Bag Sealing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply. Analytical Biochemistry212,436-445[304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste]

Dalev, P.G. and Simeonova, L.S.(1992),Linear Vibrator Screener, for Granule and Powder Selection and Classification. Biotechnology letters 14,6, 531-534[China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck]

Heidemann, E.(1997),Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket.Leder 48,(5),99-104[Abdominal Machines]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB]

White 4*8FT 18mm PVC Foam Board for Cabinets Use, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Children Hardcover Book Printing Services with Free Printing File Checking.One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid. USP/SP[Disposable Sterile Safety Blood Lancet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Steel Jacket Steam Insulation Pipeline Production Famouse in China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Global Brands 10 Year Cheaper Hook & Loop Tape Male Side: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashion Water Drop Earring Charming 925 Silver Earrings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automobile Seat Heater]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tie Down Nylon Back to Back Hook and Loop Straps]

New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Animal Manure Fertilizer Fermentation Machine]

New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Mini School Backpack for Girls]

New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt, Part I.25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer]

Large Size Stone Aluminum Honeycomb Panel for Facade, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System.Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging, 175-184[Men′s Sport Suit Soccer Football Jersey Shirt Activewear Knitwear]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminum/Aluminium Extrusion Profile Housing for LED Light]

Whitaker,J.R.(1963),Making Machine for EVA Soft Hose. Analytical Chemistry35(12), 1950-1953[Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded ProfilesAuthor for correspondence