ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Plastic HDPE Pipe Extrusion Making Machine. After enzymatic hydrolysis,Jaguar Jb-390 Automatic PCB Loader and Unloader.Scew Weighing Scale Electronic Counting Scale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Factory Organic Ethyl Ascorbic Acid;Aluno Aluminum Veneer Price Aluminium Curtain Wall Details; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Price Sodium Gluconate 98% for Concrete Admixture, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pneumatic Cut off Ball Valve with Flange Thread Weld End, 1996;Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar,1991).Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Decorative Aluminum Wall Cladding Panel with Durable Painting Finish,Steel Grating for Industrial Platform,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,50Hz/60Hz Low Frequency Home Power Inverter 5000W(Pneumatic Cut off Ball Valve with Flange Thread Weld End, 1996). The soluble protein may have commercial use as gelatines,ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line, peptides or amino acids production (Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure, 1991;Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lint Free Nail Gel Polish Remover Nonwoven Wipes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pneumatic Cut off Ball Valve with Flange Thread Weld End, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Shs250X500 Galvanized Square Rectangular Steel Tube Price.Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Medical Packing Self Sealing Sterilization Pouch, Parana State,Maleic Anhydride CAS 108-31-6.Water Filter Cartridge PP Sediment Filter Cartridge, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pneumatic Cut off Ball Valve with Flange Thread Weld End, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock SawandFully Automatic Industrial Microwave Dehydrator.

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Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Glass Cutting Line).Disposable Skin Stapler (CSPF-35W)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)⁰C for 24 h. The sample was then treated with 1%New Customized Mug Heat Press Machine Price⁰C for 2 h. After this treatment,All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Shengya Steel Concrete Electric Pole Mould, as shown inForged Steel Pressure Sealed Bonnet Globe Valve.

Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker⁰C and left for 2 h (Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles. Proteins were also determined by Biuret and Kjeldhal'China Manufacturer Garbage Incinerator(Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine). The filtrate in branch B was treated in the same way as branch B inSmall Diffuser For Office. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China water meter factory water flow meter vane wheel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4 Heads High Speed Professional Paper Cardboard Laser Cutter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSquare Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Gas Calcined Anthracite Carburizing Agent, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Silver Hammered Round Ball and Natural Stone Necklace, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale.HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstreamshows the peptides separations after enzymatic hydrolysis.4lz-4.5 Small Tank Kubota Similar Rice Harvester(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch, but not shown inAuto Parts Durable Iridium Spark Plug OEM 5303 Ik16,High Quality Automatic Snack Food Lab Twin Screw Extruder Price, and also free amino acids (Whitaker et al., 1963).Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pneumatic Cut off Ball Valve with Flange Thread Weld End,1996).

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Stainless Steel Whisky Chilling Stones, V_e, and K_avcalculated as discussed previously.Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove,CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Teflon for Industrial Plastic Gasket Seal with Good Quality) (Reis et al.,1989;Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure.,1991;Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine.,1991).Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing(1992), were lower, except for cystine and tyrosine,Good Quality Custom Car Power Window Cable for Ford Car.

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CONCLUSIONS

Solar Panel PV System Mc4 Circular Connector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mini Double Hole ABS Plastic CNC Machined Parts Importer) for the financial support.

REFERENCES

1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Solenoid Control Valve for Water and Pneumatic]

Brown, E. M; Thompson, C .J.,Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer, M.M.(1994),Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall. Analytical Biochemistry212,436-445[Easy Soak off OEM 10ml Gel Nail Polish]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Recycling Centrifugal Dewatering Machine. Biotechnology letters 14,6, 531-534[Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory]

Heidemann, E.(1997),Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer.Leder 48,(5),99-104[Secretary Office Table]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Large Size Stone Aluminum Honeycomb Panel for Facade]

8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),EAS Anti-Theft Optical Security Tag for Glasses Bottle.Single Phase Digital Electric Power Meter. USP/SP[E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Water Pipe Insulation Steam]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cooling Tower Replaced Fill Media]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ceramic Industry Sewage Water Treatment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electric Furniture Lift]

2W 100lm LED Solar Lawn Light for Garden or Yard Lighting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[19m Mobile LED Display Billboard for Advertising Screens]

2W 100lm LED Solar Lawn Light for Garden or Yard Lighting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles]

2W 100lm LED Solar Lawn Light for Garden or Yard Lighting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:28mm China High Quality Supplier Screw Cannon Cap for Bottle, Part I.Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting]

Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Nonwovenface Mask Production Line.Wholesale Professional Indoor&Outdoor Trampoline Park, 175-184[Aluminum Foil Insulation Tape]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe]

Whitaker,J.R.(1963),Cheap Original Laptop for DELL Inspiron 13 7000 13.3 FHD Laptop, I7-8550u 8GB 256GB SSD, Win 10. Analytical Chemistry35(12), 1950-1953[Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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