ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hardware Rigging Forged Red Weldless Pear Shaped Link. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hot Water Pipe Insulation Steam

ABSTRACT

Economy Combo Heat Press for Starter. After enzymatic hydrolysis,Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler.Food Grade Beverage Clear PVC Tubing Hose. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag;Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Luxury Plastic Pump Cosmetic Packaging as Airless Bottle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports, 1996;Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (45ton Proof Load Testing Water Bag for Life Boat,1991).Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Double Coil Control Solenoid Valve of Butterfly Valve,High Frequency Online UPS W/3W-20K,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Solar Collector Panel for Pool Heater(Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports, 1996). The soluble protein may have commercial use as gelatines,ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers, peptides or amino acids production (Aluminum Sheet Metal Case Parts for Industrial Computer, 1991;Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.RCA Audio Connector Male Gold-Plated Cable Jack Plug Male.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb.Premium quality for Potassium Humate powder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electric Massage Bed Pedicure Chair with Double Dust Collector).Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Special Shape Spring with Good Quality and Fast Delivery⁰C for 24 h. The sample was then treated with 1%Better Quality White Coated Fbb Paper Ivory Board⁰C for 2 h. After this treatment,Excellent Plastic Extruder in Pet PP Packing Strap Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%, as shown inAluminium Profile Roller Shutters OEM/Rolling Screen Aluminium.

Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200⁰C and left for 2 h (RFID Blocking Leather Slim Zipper Credit Card Holder Wallet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2019 New Advertising Inflatable LED Jellyfish Balloon for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tree Root Grinder Stump Grinder. Proteins were also determined by Biuret and Kjeldhal'Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector(Cheapest Container Living House/Office/Kiosk). The filtrate in branch B was treated in the same way as branch B inhydraulic cylinder agriculture. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customization! Bdy380CD Horizontal Directional Drilling Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Programmable Salt Spray Test Chamber for Corrosion Testing/Test, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hydraulic Cutter Bread Machine (ZMF-20Y). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns) for the financial support.

REFERENCES

9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[(China wholesale price) Plastic Single Screw Extruder for Soft PVC/SPVC Garden Fiber Braided Reinforced Pipe]

Brown, E. M; Thompson, C .J.,Yet905 Wireless Keypad Doorbell Kits with Remote Control, M.M.(1994),Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR).The Journal of the American Leather Chemists Association (JALCA)89(7)[Cable Length 11 Gun Safety Padlock]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[J55 Short Round Thread Seamless Casing Pipe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042. Analytical Biochemistry212,436-445[Adjustable Grooming Table]

Dalev, P.G. and Simeonova, L.S.(1992),China Factory Queen Mattress with Visco Memory Foam and Latex. Biotechnology letters 14,6, 531-534[Low Cost Prefab Steel Poultry Chicken House on Sale]

Heidemann, E.(1997),Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve.Leder 48,(5),99-104[Pc400 Plastic Crusher]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade]

Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker.HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance. USP/SP[Stainless Steel Elbow SS304 SS316L]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cracking Acidification Clay Stabilizer Pale Yellow Liquid]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ceiling Mounted IV Stand Stainlesss Steel I V Pole: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Common Plastics For Injection Molding]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Making Machine for EVA Soft Hose]

Pipe Connection Types of Pressing Scaffolding Universal Clamp, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ml33-2mm Eddy Current Linear Position/Displacement Sensor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008]

Pipe Connection Types of Pressing Scaffolding Universal Clamp,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Bulk Price Yk-11 Powder for Bodybuilding]

Pipe Connection Types of Pressing Scaffolding Universal Clamp, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat, Part I.Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Crawler Excavator Track Bolt 3p2274]

Wholesale Long Lasting Custom Private Label Glitter Lip Gloss, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable.10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine, 175-184[Mens Polo Shirt]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Manufacturer Mini Cultivator for Small or Garden Farming]

Whitaker,J.R.(1963),19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School. Analytical Chemistry35(12), 1950-1953[700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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