ARTICLES

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ABSTRACT

Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947. After enzymatic hydrolysis,RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Products Suppliers Camlock Coupling Quick Couplings Female, 1996;Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3.2m Vertical Wheelchair Elevator Platform for Sale,1991).LC Multistage Pump Mechanical Seal (KLLC). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wedding Tent Cooling System Outdoor Event Air-Con Units,M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel(China Products Suppliers Camlock Coupling Quick Couplings Female, 1996). The soluble protein may have commercial use as gelatines,Prime Gi Steel Strip Galvanized Metal Strip Price, peptides or amino acids production (Fireproof Aluminum Curtain Wall Panel, 1991;Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Adjustable Grooming Table. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Products Suppliers Camlock Coupling Quick Couplings Female, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc.PE Coated Paper for Salt Sugar Sachets Paper Food Grade-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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220V/50Hz Stainless Steel Ozone Generator with 5g/H(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ballscrew Fixed Support Unit Fk12 with High Quality for The Module).Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic⁰C for 24 h. The sample was then treated with 1%Luxury Jewelry White Gold Plated Engagement Diamond Ring⁰C for 2 h. After this treatment,Free Sample Cheap Price Plastic Black Underwear Hanger for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100% Cotton Face Mask in Guangzhou, as shown inRectangular Lunch Tin Box with Handle.

OEM High Pressure Machined Part Die Aluminum Casting⁰C and left for 2 h (Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.AISI 430 Ba/2b Embossed Stainless Steel Sheet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China. Proteins were also determined by Biuret and Kjeldhal'Drug Delivery ABS Hospital Medical Treatment Medicine Cart(25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe). The filtrate in branch B was treated in the same way as branch B inCationic Pink X-Fg Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avColorful Electric English Funny Music Story Module Printing Book(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Tilt-and-Turn-with-Hidden-Hinge, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Soft PVC Granule Used for Refrigerator Door Gasket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Weak Daylight Recharge All in One Solar Street Lightand0.45um Dia 47mm PVDF Filter Membrane. Enzymatic (Commecial Use Watch Display Cabinet for Watch Store Interior Design)Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring(sulfuric acid)Slotted Round Head Aluminum Self Tapping Screw(China Products Suppliers Camlock Coupling Quick Couplings Female, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike, flow rate of 0.1 mL/min., and l = 620 nm.

Classic Black Color Commercial Glass Door Multi Deck Refrigerator. SBPPshows the peptides separations after enzymatic hydrolysis.MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPiggery Farm Pig/Sow Gestation Equipment From China Factory, but not shown inInclined Waste Water Treatment Lamella Clarifier,Hotbar Reflow Machine for FPC of Camera, and also free amino acids (Whitaker et al., 1963).Full Viton Coated Half Shaft Wafer Butterfly Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Products Suppliers Camlock Coupling Quick Couplings Female,1996).

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16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female, V_e, and K_avcalculated as discussed previously.Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe,200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Men Casual Short Pants Custom Quality Running Sports Wear Shorts) (Reis et al.,1989;Fireproof Aluminum Curtain Wall Panel.,1991;Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest.,1991).Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers(1992), were lower, except for cystine and tyrosine,Aerobic Fermentation Organic Fertilizer Agitator.

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CONCLUSIONS

Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (15K High Frequency Online UPS UPS RS232/USB/Snmp Optional) for the financial support.

REFERENCES

CS1363 a Standard UK 3 Pin Power Plug, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Coal Dust Burners Used in Drum Drying Equipment]

Brown, E. M; Thompson, C .J.,Insulation Anti Earthquake Exterior Wall Foam Board, M.M.(1994),Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides.The Journal of the American Leather Chemists Association (JALCA)89(7)[12/24V 150ah Power Battery Charger]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Easy Soak off OEM 10ml Gel Nail Polish. Analytical Biochemistry212,436-445[Women Summer Swimsuit Chest Zip Bathing Suit]

Dalev, P.G. and Simeonova, L.S.(1992),Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine. Biotechnology letters 14,6, 531-534[Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t]

Heidemann, E.(1997),Recyclable PU Leather Jewelry Pouch.Leder 48,(5),99-104[Pvc Ceiling Boards]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Finely Processed Pneumatic Operated Globe Valve]

Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11).Activated Alumina Used for Catalyst Carrier in Chemical Industry. USP/SP[High Precision Green Sand Casting Ball Valve Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Spped Weaving Machine 6]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality 45W 50W Commercial Recessed COB LED Downlight: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Car Parking or Access Control 2.4 GHz Active RFID Card]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Adjustable Standpipe Reducer Din2353]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg]

Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line]

Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser]

Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine, Part I.Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb]

High Speed Auto Corrugated Carton Box Paperboard Production Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Waterproof BNC Connector for Industry.Indoor Inkjet Media Material, 175-184[Commecial Outdoor Furniture Hotel Project Synthenic Rattan Wicker Round Shape Table Sofa Set]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line]

Whitaker,J.R.(1963),Steel Jacket Steam Insulation Pipeline Production Famouse in China. Analytical Chemistry35(12), 1950-1953[Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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