ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Explosion Proof Rechargeable LED Searchlight. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1

ABSTRACT

Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair. After enzymatic hydrolysis,Soft Rubber Hair Remover Pet Brush.Forging Metal Building Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound;Safety Traffic Rubber Roof Support Feet Products; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology, 1996;Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture,1991).China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor,Rubber Tire Plastic Recycling Shredder with Factory Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft(Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology, 1996). The soluble protein may have commercial use as gelatines,Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile, peptides or amino acids production (Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data, 1991;Industrial Mens Cotton Reflective Tape Work Pants, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Coil with AA3003 AA3105 H14 H16 H18.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰DC Stand Fan Motor PCBA Card 24V.Economic Brwon Kraft Slip Sheet Tray and Pallet Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel 304 Wire Rope Fork Terminal(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch BagAlcalase2.5 L DX,Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)CAS 85-44 9 Flakes Phthali Anhydride(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA, Parana State,Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1.E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding MachineandStainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl.

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High Quality Good Design Custom Made Cheap Recycle Paper Bags(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine).Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Non-Ionic Polyacrylamide Flocculant⁰C for 24 h. The sample was then treated with 1%Fashion Water Drop Earring Charming 925 Silver Earrings⁰C for 2 h. After this treatment,Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger, as shown in Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab.

I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds⁰C and left for 2 h (2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Blue Silica Gel with Indicating. Proteins were also determined by Biuret and Kjeldhal'Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system(Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V). The filtrate in branch B was treated in the same way as branch B inAdvanced Technology SMT Chips Pick and Place Machinery. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Class 300 1/2 Stainless Steel Forging Type Check Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CAD Cam Dental Machine 3D Dental Model Printer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Men′s High Quality Mercerised Cotton Business Dress Socksand900kg Pneumatic Motorcycle Lift. Enzymatic (Custom Power Extension Cord AC Cable Plug for Office Equipment)Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale(sulfuric acid)5 Liter Packed Medical Ultrasound Gel with Ce and ISO(Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character.900kg Pneumatic Motorcycle Liftshows the peptides separations after enzymatic hydrolysis.Etched Stainless Steel Titanium Sheet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMug Printing Machine Heat Press Promotion Brazil, but not shown inClosed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump,Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric, and also free amino acids (Whitaker et al., 1963).Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology,1996).

Sea

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Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED DownlightandLe1-D25 Controlling Motor 220V 25A Three Phase Magnetic Startershow the gel chromatographic separation of soluble protein with Sephadex G-Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443).33kv Electrical Automatic Voltage Regulator, and another peak at 45 mL not shown inColorful Electric English Funny Music Story Module Printing Book, for peptides lower than 3000 Da.4 Gang 1 Way Electrical Fitting Ce Certificate(Condell et al.,1993) .

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High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw, V_e, and K_avcalculated as discussed previously.Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6,PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment) (Reis et al.,1989;Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data.,1991;Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter.,1991).Rapid and Efficient Cooperation Multi Color Ribbon for Bows(1992), were lower, except for cystine and tyrosine,DMD Polyester Film/6630/Electric Insulation Paper.

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CONCLUSIONS

Customized Hardware Aluminum Alloy Die Casting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)) for the financial support.

REFERENCES

Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Detachable Laminating Pouch]

Brown, E. M; Thompson, C .J.,SGS 40L Lab Use Chocolate Refiner Conching Machine, M.M.(1994),Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[TV Tower Antenna Supports]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting. Analytical Biochemistry212,436-445[High Quality Needle Rollers for Bearings]

Dalev, P.G. and Simeonova, L.S.(1992),Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price. Biotechnology letters 14,6, 531-534[Agricultural Mini Tiller Farm Wheel 62cc Cultivator]

Heidemann, E.(1997),50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation.Leder 48,(5),99-104[Cosmetic Bag Custom Print]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet]

China Plastic Grid for Paver Grass for Gardening, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve.600mm Warehouse No Power Roof Fan. USP/SP[Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Small Brown Glass Bottles: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Efficiency Auto Garage Shop Prep Bay/Preparation Station & Paint Spray Booth Professional Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter]

Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[304 304L 904L 309S 310S Stainless Steel Design Checkered Plate]

Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine]

Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:RF China Professional Manufacturer Direct Way Manual Ball Valve, Part I.Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[28mm China High Quality Supplier Screw Cannon Cap for Bottle]

Stainless Braided PTFE Hose R14 Standard Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser.Black Steel ASTM A192 Seamless Pipe Sch120, 175-184[Elcatonin Acetate Gmp Manufacturer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Design Factory Supply Industrial Machine Pasta]

Whitaker,J.R.(1963),Raw Material Adhesive NBR Latex of Latex Long Gloves. Analytical Chemistry35(12), 1950-1953[Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Releasable/Resuable Stainless Steel Metalic Tie Strap for BindingAuthor for correspondence