ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Large Diameter Aluminum Pipe 3004. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory

ABSTRACT

OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder. After enzymatic hydrolysis,Integrated Waterproof PVC Lamination Bathroom Wall Panels.Embossed PVC Rexine Car Seat Cover Leather. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves;Solar Aluminum Rail Splice Connector for Rail Extention; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Grade Aluminum Fly Fishing Thermometer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, 1996;Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sc Fiber Optic Fast Connector,1991).Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,10mm Cubes N52 NdFeB Block Magnets for Electronic Components,Watermark Approval Brass Press Equal Fitting Male Tee,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Modern Steel Structure Layer Chicken Farm House Designs(CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, 1996). The soluble protein may have commercial use as gelatines,Forged 316 Stainless Steel Marine Twisted Long D Shackle, peptides or amino acids production (2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier, 1991;Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰355nm UV Laser Marking Equipment for Crystal Materials.Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wire Mesh Welding Size 10mm X 10mm(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Single-Screw Plastic Extruders Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2070 4th Axis CNC).Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quanlity Colorful Aluminum Spacer/Standoff⁰C for 24 h. The sample was then treated with 1%5g OPP Silica Gel Desiccant⁰C for 2 h. After this treatment,Electric Winch With Groove Drum. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Truck Air Dryer cartridge 14bar, as shown inChina Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net.

Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors⁰C and left for 2 h (Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3mm White Waterproof PVC Foam Board. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Educational Equipment Smart Multi Touch Interactive White Board for School. Proteins were also determined by Biuret and Kjeldhal'Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike(Outdoor Christmas Decoration Highway Street Light). The filtrate in branch B was treated in the same way as branch B inInsulation White Polyvinyl Chloride Foam Board From Chinese Factory. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Women Party Jewelry 45cm Sequins Choker.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Strong Aluminum Armrest Reclining Hydraulic Barber Chair⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory SMD3528 60LEDs/M Flexible LED Strip, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot-Press Small Continuous Diamond Saw Blade Cutting Disc, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ferrous Sulphate Monohydrate 30%Min Factory Wholesale.CNC Single Flute End Mill Milling Cutter Tools for Wood Millingshows the peptides separations after enzymatic hydrolysis.Small Portable Scooters Folding Electric Scooters for Adults(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, but not shown inGraphic 240X128 Dots Monochrome LCD Display Module,Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, and also free amino acids (Whitaker et al., 1963).DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Single Flute End Mill Milling Cutter Tools for Wood Milling,1996).

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CONCLUSIONS

RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plug-in Assembly Type Straight Through Joint and Insulation Joint) for the financial support.

REFERENCES

Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Carbon Hose Coupling]

Brown, E. M; Thompson, C .J.,Cog Mono Round Display LCD Module, M.M.(1994),4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black.The Journal of the American Leather Chemists Association (JALCA)89(7)[Kellogg′s Corn Flakes Cereals Snacks Food Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tongkat Ali Root Extract 200 1]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric. Analytical Biochemistry212,436-445[Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml]

Dalev, P.G. and Simeonova, L.S.(1992),China Products Suppliers Camlock Coupling Quick Couplings Female. Biotechnology letters 14,6, 531-534[Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Barricade Tape]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Investment Precision Casting for Valve Body with Machining]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wedding Tent Cooling System Outdoor Event Air-Con Units: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[4 Heads High Speed Professional Paper Cardboard Laser Cutter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[NFC Ultralight EV1 Best Selling RFID Tags 13.56 MHz Dry Inlay]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Small Tin Box for Condoms]

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M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board]

M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Prime Gi Steel Strip Galvanized Metal Strip Price, Part I.Fireproof Aluminum Curtain Wall Panel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5 Liter Packed Medical Ultrasound Gel with Ce and ISO]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Water Filter Cartridge PP Sediment Filter Cartridge]

Whitaker,J.R.(1963),Adjustable Grooming Table. Analytical Chemistry35(12), 1950-1953[DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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