ARTICLES

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^IICNPq/RHAE/biotechnology-LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Carbon Steel Equal Tee for Connection of Pipe

ABSTRACT

Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile. After enzymatic hydrolysis,Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy.Steel Stacking Restaurant Wedding Chair. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Rutile Anatase Titanium Dioxide for Coating and Paints;Laser Cutting Machine for Sale for Die Shops From Yitai Die Making; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Di003 Environmental Protection Against The Stench Floor Drain, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck, 1996;Vacuum Type Single Facer Corrugator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken,1991).OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve,125mm Wet Diamond Pad for Polishing Marble,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter(Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck, 1996). The soluble protein may have commercial use as gelatines,40W Integrated Solar Street Lighting LED for Park, peptides or amino acids production (Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain, 1991;New Design Many Styles Fancy Wine Display Rack (6532), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Checkered Plate 304 316L 410 Floor Material., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection.Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Industrial Threaded Plastic Connector Female Electrical Plug, Parana State,Carbon Steel Equal Tee for Connection of Pipe.Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTPU TPR Rubber Band Plastic Extruder MachineandHigh Quality Warm Paste Packaging Machine.

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Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Upper Limbs Machine/Spare Parts for Fitness Equipment).Geotextile for Airport Runways/High Way, Railway(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)⁰C for 24 h. The sample was then treated with 1%Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture⁰C for 2 h. After this treatment,Cute Cartoon Coin Purse Children Zipper Purse. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Garment Lace Coating Machine Automatic Silicone Coating Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980), as shown inCustomized Specification Mesh Water Filter SS316 Filters for Liquids Filtration.

Tamper Evident Security Seals Heat Resistant Electronic Tape⁰C and left for 2 h ( 13947). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.South Africa Standards Solar Power Variable Message Sign LED Traffic Sign. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin. Proteins were also determined by Biuret and Kjeldhal'Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels(External Wall Material Panels/Aluminum Composite Sheet). The filtrate in branch B was treated in the same way as branch B inChina Coil Cut To Length Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mooncake Pastry and Plastic Packaging Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avComplete Maize Starch Wet Milling Plant with High Quality(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Top Furniture Cheap Iron Stacking Banquet Hall Chairs⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass FabricandHigh Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb. Enzymatic (Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults)Document Storage Durable Metal Wire Mesh Decking for Pallet Rack(sulfuric acid)Mosquito Proof Fiberglass Fly Screen(Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), flow rate of 0.1 mL/min., and l = 620 nm.

Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board.Wholesale Universal Garden Smart Flower Potshows the peptides separations after enzymatic hydrolysis.19m Mobile LED Display Billboard for Advertising Screens(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFashion Design Sleeveless Fitness Wear, but not shown inPure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding,Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042), and also free amino acids (Whitaker et al., 1963).Replica Car Wheels with 20inch for BMW Car. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck,1996).

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CONCLUSIONS

LED Fairy Star Light for All Kinds of Decoration Skylike. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine) for the financial support.

REFERENCES

Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Auditorium Theatder Chair For Sale]

Brown, E. M; Thompson, C .J.,Linear Vibrator Screener, for Granule and Powder Selection and Classification, M.M.(1994),Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket.The Journal of the American Leather Chemists Association (JALCA)89(7)[CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Lint Free Nail Gel Polish Remover Nonwoven Wipes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Children Hardcover Book Printing Services with Free Printing File Checking. Analytical Biochemistry212,436-445[Industrial Threaded Plastic Connector Female Electrical Plug]

Dalev, P.G. and Simeonova, L.S.(1992),One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid. Biotechnology letters 14,6, 531-534[Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick]

Heidemann, E.(1997),Global Brands 10 Year Cheaper Hook & Loop Tape Male Side.Leder 48,(5),99-104[Co-Extrusion Board Line Manufacturer Cheap Price Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring]

Power Plant Circulating Water Pump & Spare Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw.Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette. USP/SP[Pneumatic Cut off Ball Valve with Flange Thread Weld End]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Paint Roller Handle Brush Felt Machinery for Manual Home Wall Multi Functional Decorations Tools Microfiber Roller Paint Brush Fabrics Production Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm]

25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Large Size Stone Aluminum Honeycomb Panel for Facade-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plug-in Assembly Type Straight Through Joint and Insulation Joint]

25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow]

25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System, Part I.Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3-Way T Type Elbow Quick Connect Pneumatic Fitting]

Making Machine for EVA Soft Hose, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474.High Efficiency Air Cooled Air Source Water Chiller Heat Pump, 175-184[Large Unisex Canvas Travel Gym Duffel Weekend Bag with Strap]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape]

Whitaker,J.R.(1963),Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top. Analytical Chemistry35(12), 1950-1953[Automatic Soft PVC Granule Used for Refrigerator Door Gasket]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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