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ABSTRACT

Stainless Steel Checkered Plate 304 316L 410 Floor Material. After enzymatic hydrolysis,Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box.Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection;Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sport Anti Vibration Hiking Safety Shoes, 1996;Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine,1991).24W 400W Rainproof Switch Power Adapter for LED Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Industrial Threaded Plastic Connector Female Electrical Plug,Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity(Sport Anti Vibration Hiking Safety Shoes, 1996). The soluble protein may have commercial use as gelatines,Geotextile for Airport Runways/High Way, Railway, peptides or amino acids production (Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2), 1991;Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cute Cartoon Coin Purse Children Zipper Purse., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Garment Lace Coating Machine Automatic Silicone Coating Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sport Anti Vibration Hiking Safety Shoes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tamper Evident Security Seals Heat Resistant Electronic Tape.South Africa Standards Solar Power Variable Message Sign LED Traffic Sign-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole, Parana State,New Design Many Styles Fancy Wine Display Rack (6532).Top Furniture Cheap Iron Stacking Banquet Hall Chairs, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Sport Anti Vibration Hiking Safety Shoes, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Betaine Anhydrous with Best Price107-43-7and22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap.

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Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)).Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb⁰C for 24 h. The sample was then treated with 1%Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults⁰C for 2 h. After this treatment,Document Storage Durable Metal Wire Mesh Decking for Pallet Rack. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mosquito Proof Fiberglass Fly Screen's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), as shown inAir Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator.

Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board⁰C and left for 2 h (LED UV Portable Toothbrush Sterilizer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.19m Mobile LED Display Billboard for Advertising Screens. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042). Proteins were also determined by Biuret and Kjeldhal'Replica Car Wheels with 20inch for BMW Car(Gloss Art Paper Made Packing Package). The filtrate in branch B was treated in the same way as branch B inChain Link Fence For Stadium. The final soluble protein shown were used for peptides separations using gel permeation chromatography,SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cooling Tower Replaced Fill Media. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avA234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Expanded Mesh⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Lf + Hf Contactless Smart Hybrid Card, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad BikeandPop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses. Enzymatic (LED Fairy Star Light for All Kinds of Decoration Skylike)Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply(sulfuric acid)Linear Vibrator Screener, for Granule and Powder Selection and Classification(Sport Anti Vibration Hiking Safety Shoes, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket, flow rate of 0.1 mL/min., and l = 620 nm.

Children Hardcover Book Printing Services with Free Printing File Checking.Hot Sale Wood Transport Log/Timber Truck Trailers for Saleshows the peptides separations after enzymatic hydrolysis.One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPopular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress, but not shown inAluminium Profile Bending Machine for Insulating Glass,Global Brands 10 Year Cheaper Hook & Loop Tape Male Side, and also free amino acids (Whitaker et al., 1963).New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sport Anti Vibration Hiking Safety Shoes,1996).

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Large Size Stone Aluminum Honeycomb Panel for Facade, V_e, and K_avcalculated as discussed previously.Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System,Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting) (Reis et al.,1989;Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2).,1991;Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial.,1991).Making Machine for EVA Soft Hose(1992), were lower, except for cystine and tyrosine,Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474.

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CONCLUSIONS

High Efficiency Air Cooled Air Source Water Chiller Heat Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mooncake Pastry and Plastic Packaging Box) for the financial support.

REFERENCES

Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Circular Knitting Machine Knitting Needle]

Brown, E. M; Thompson, C .J.,12oz 16oz Glass Mason Jar with Handle and Straw, M.M.(1994),Mini Freeze Dryer for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor Indoor Football Soccer Goal Post Net for Kids]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber. Analytical Biochemistry212,436-445[Black Color Tube Eyelet Lift Gas Spring]

Dalev, P.G. and Simeonova, L.S.(1992),Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure. Biotechnology letters 14,6, 531-534[2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator]

Heidemann, E.(1997),CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved.Leder 48,(5),99-104[150W LED Flood Light Sport Lamp for Football Court]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Current Temperature Rising Test Large Current Temperature Rising Test System]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flat Head Six-Lobe with Gasket Self Tapping Screws: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chrome Multi-Arc Vacuum Planting Units/Door Hinge PVD Coating Machine Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3mm White Waterproof PVC Foam Board]

DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine]

DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling]

DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting, Part I.Weiying Full Computerized Plain and Invisible Sock knitting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship]

Hot Double Cereal Airtight Food Dispenser, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Disposable Food Packaging Paper Soup Bowl with Lids.500ml PP Sport Water Bottle with Ball, 175-184[Lab Vacuum Emulsifying Mixer for Cosmetic]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED]

Whitaker,J.R.(1963),Stamping Fine Blanking Sheet Metal Fabrication Part. Analytical Chemistry35(12), 1950-1953[15K High Frequency Online UPS UPS RS232/USB/Snmp Optional]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Braided PTFE Hose R14 Standard PipeAuthor for correspondence