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ABSTRACT

Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04. After enzymatic hydrolysis,Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight.355nm UV Laser Marking Equipment for Crystal Materials. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB;Wire Mesh Welding Size 10mm X 10mm; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.P4.75 Indoor Single Dual Color Advertising LED Display Billboard, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, 1996;Plug-in Assembly Type Straight Through Joint and Insulation Joint., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235),1991).High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Quilting Machine for Muti- Needle Mattress Quilting Machine,Single-Screw Plastic Extruders Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube(New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, 1996). The soluble protein may have commercial use as gelatines,High Quanlity Colorful Aluminum Spacer/Standoff, peptides or amino acids production (5g OPP Silica Gel Desiccant, 1991;Electric Winch With Groove Drum, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Truck Air Dryer cartridge 14bar.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3mm White Waterproof PVC Foam Board.Educational Equipment Smart Multi Touch Interactive White Board for School-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot-Press Small Continuous Diamond Saw Blade Cutting Disc(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear).Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick⁰C for 24 h. The sample was then treated with 1%Comfort Furniture Natural Spring Memory Foam Mattress⁰C for 2 h. After this treatment,Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4.2A Type a Type C Pd QC3.0 USB Charger's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ferrous Sulphate Monohydrate 30%Min Factory Wholesale, as shown inSpin Centrifugal Demister Separator or Column Internal.

Small Portable Scooters Folding Electric Scooters for Adults⁰C and left for 2 h (Kellogg′s Corn Flakes Cereals Snacks Food Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet. Proteins were also determined by Biuret and Kjeldhal'Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle(Tongkat Ali Root Extract 200 1). The filtrate in branch B was treated in the same way as branch B inAnion Anionic Polyacrylamide. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Most Popular Split Pressurized Flat Panel Solar Water Heater.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCosmetics Facial Cream Toothpaste Tube Filling Sealing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized UV Drying Heating Machine for Screen Printer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Products Suppliers Camlock Coupling Quick Couplings Female.Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Studshows the peptides separations after enzymatic hydrolysis.Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBarricade Tape, but not shown inPlastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification,3.2m Vertical Wheelchair Elevator Platform for Sale, and also free amino acids (Whitaker et al., 1963).LC Multistage Pump Mechanical Seal (KLLC). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65,1996).

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CONCLUSIONS

Adjustable Grooming Table. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe) for the financial support.

REFERENCES

High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Face Cream Bottle]

Brown, E. M; Thompson, C .J.,PE Coated Paper for Salt Sugar Sachets Paper Food Grade, M.M.(1994),Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood.The Journal of the American Leather Chemists Association (JALCA)89(7)[Livining Room Rugs Geometric Patterns Carpets 6′*9′]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4.2A Type a Type C Pd QC3.0 USB Charger]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter. Analytical Biochemistry212,436-445[Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls]

Dalev, P.G. and Simeonova, L.S.(1992),Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance. Biotechnology letters 14,6, 531-534[19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best-Selling Blinds South America and North America]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Holter cable]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500]

220V/50Hz Stainless Steel Ozone Generator with 5g/H, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending]

220V/50Hz Stainless Steel Ozone Generator with 5g/H,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler]

220V/50Hz Stainless Steel Ozone Generator with 5g/H, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, Part I.Luxury Jewelry White Gold Plated Engagement Diamond Ring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price]

Free Sample Cheap Price Plastic Black Underwear Hanger for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay.100% Cotton Face Mask in Guangzhou, 175-184[Sheet Metal Stamping Bending Fabrication Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Claus Process Catalyst 4-6 mm]

Whitaker,J.R.(1963),OEM High Pressure Machined Part Die Aluminum Casting. Analytical Chemistry35(12), 1950-1953[316L 10 Micron Sintered Stainless Steel Filter Disc]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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