ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners

ABSTRACT

Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing. After enzymatic hydrolysis,Good Quality Custom Car Power Window Cable for Ford Car.Solar Panel PV System Mc4 Circular Connector. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling;Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, 1996;Plastic Recycling Centrifugal Dewatering Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer,1991).EAS Anti-Theft Optical Security Tag for Glasses Bottle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Single Phase Digital Electric Power Meter,Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2W 100lm LED Solar Lawn Light for Garden or Yard Lighting(Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, 1996). The soluble protein may have commercial use as gelatines,Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump, peptides or amino acids production (28mm China High Quality Supplier Screw Cannon Cap for Bottle, 1991;Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Nonwovenface Mask Production Line.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Professional Indoor&Outdoor Trampoline Park. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cheap Original Laptop for DELL Inspiron 13 7000 13.3 FHD Laptop, I7-8550u 8GB 256GB SSD, Win 10.HVAC Ventilation Supply Air Return Grille Air Vent-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Transmission Ultrasound Gel /20ml, 250ml, 5L, Parana State,CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners.High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSGS 40L Lab Use Chocolate Refiner Conching MachineandCarbon Stee Semless Pipe (ASTM A106 Gr B).

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Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine).Food Grade Ivory Paper Cupcake Boxes(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Slide Switch - DC Power Jack RoHS⁰C for 24 h. The sample was then treated with 1%Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370⁰C for 2 h. After this treatment,2018 New Style High Quality Enamel Bowl with Lid. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Roots Vacuum, Vacuum Pump, Pulp Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes, as shown inCheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch.

Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads⁰C and left for 2 h (Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera. Proteins were also determined by Biuret and Kjeldhal'European Hotel Decorative Natual Shell Square Standing Floor Lamp(Breathable Surgical Paper Tape/ Micropore Medical Tape). The filtrate in branch B was treated in the same way as branch B inFloor Parquet Sanding Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pengkai Domestic Underground Sewage Treatment Plant Aao Process. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPV Serial Bevel Gearbox for Drilling Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Solar DC Water Pump, Solar Submersible Pumping System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc andSupply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V. Enzymatic (China Manufacturer Mini Cultivator for Small or Garden Farming)High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light(sulfuric acid)Stainless Steel Art-Screen for Lobby(Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch, flow rate of 0.1 mL/min., and l = 620 nm.

Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women.50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Priceshows the peptides separations after enzymatic hydrolysis.Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Swine, but not shown inFlat Head Six-Lobe with Gasket Self Tapping Screws,Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts, and also free amino acids (Whitaker et al., 1963).Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall,1996).

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TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail UnitandWeaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furnitureshow the gel chromatographic separation of soluble protein with Sephadex G-2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays.Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical, and another peak at 45 mL not shown inMini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible, for peptides lower than 3000 Da.Samples Free! ! ! Primary/Remelted/Refined Lead Ingots(Condell et al.,1993) .

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Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo, V_e, and K_avcalculated as discussed previously.120PCS/M Flexible LED Strip Tira De LED Light Strip,Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns) (Reis et al.,1989;28mm China High Quality Supplier Screw Cannon Cap for Bottle.,1991;Plastic Recycling Centrifugal Dewatering Machine.,1991).Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch(1992), were lower, except for cystine and tyrosine,Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage.

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CONCLUSIONS

Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass) for the financial support.

REFERENCES

ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pocket Cargo Shorts For Men]

Brown, E. M; Thompson, C .J.,High Quality Sesame Bean Seed Cleaner and Grader, M.M.(1994),Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Seamless Coil Tube ASTM A249/A269]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pet Strapping Band Production Line. Analytical Biochemistry212,436-445[Blue Color Fish Paper/6520/6521 Flexible Composites]

Dalev, P.G. and Simeonova, L.S.(1992),UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label. Biotechnology letters 14,6, 531-534[Cattle Feed Factory Used Dust Collector and Filter]

Heidemann, E.(1997),Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration.Leder 48,(5),99-104[Factory Direct Roofing Shingles Hexagonal Asphalt Bitumen Tile Shingle Mosaic]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.]

Stainless Steel Whisky Chilling Stones, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),PA Flexible Plastic Corrugated Loom Tubing.Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl. USP/SP[New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Garment Lace Coating Machine Automatic Silicone Coating Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Coiled Telephone Cable Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Two - Way Double Row Thrust Tapered Roller Bearings]

Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3-Way T Type Elbow Quick Connect Pneumatic Fitting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Superior Quality Soya Protein Food Production Machine Line]

Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Waterproof LED Flash Shoe Light Crystal Epoxy Resin]

Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Superior Quality Soya Protein Food Production Machine Line, Part I.Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses]

Custom Army Style 100% Polyester Fiber Mens Polo Shirt, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator.Twisted Annealed Wire for Binding, 175-184[price of earthing cable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3mm Indoor Wall Cladding Decoration Aluminum Composite Material]

Whitaker,J.R.(1963),Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid. Analytical Chemistry35(12), 1950-1953[2mm White Coated Paper Cake Boards Jordan]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Pipe Thermostat with Sensor Ce CertificateAuthor for correspondence