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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (14 Gauge Twist Galvanized Black Annealed Steel Wire, 1996;2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fully Automatic Digital Pid Temperature Controller,1991).High Quality Anti-Corrosion Fiberglass Sheet and Roll. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter,Full-Automatic Folding Napkin Paper Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Frame Mount Highway Pegs Enclosure Parts(14 Gauge Twist Galvanized Black Annealed Steel Wire, 1996). The soluble protein may have commercial use as gelatines,Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling, peptides or amino acids production (10mm Tempered Bathroom Glass Safety Glass for Building, 1991;3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cattle Feed Factory Used Dust Collector and Filter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Group 152cm Picnic Folding in Half Table. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (14 Gauge Twist Galvanized Black Annealed Steel Wire, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tactile Custom Branded Membrane Switch Keyboard with Foil.304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Medical Cable Assembly Wire Harness Manufacturer).Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pickled Cucumber Production Line/Making Machine⁰C for 24 h. The sample was then treated with 1%Rectangular Lunch Tin Box with Handle⁰C for 2 h. After this treatment,Inflatable Cheering Stick for Promotional Gift. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook, as shown inASTM A335 Seamless Steel Pipe by Grade P91.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avindustrial aluminum foil roll 8011 8079 1235(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Afraic Market Mobile Food Vending Cart, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Australia Two Pins Power Cord with SAA Certification) for the financial support.

REFERENCES

2017 Hot Diped Galvanized Welded Gabion Basket, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[0280156318 Fuel Injector for Peugeot Citroen 1.6L High Impedance]

Brown, E. M; Thompson, C .J.,Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash, M.M.(1994),100%Rayon Red Satin Dyeing Fabric for Women Dress.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Agrochemicals]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Small Brown Glass Bottles]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Promotional Gifts Customized Woven Polyester Lanyard for VIP Card. Analytical Biochemistry212,436-445[Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins]

Dalev, P.G. and Simeonova, L.S.(1992),High Density Artificial Grass Football Playground Surfaces (STO). Biotechnology letters 14,6, 531-534[High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB]

Heidemann, E.(1997),High Quality Male Water Quick Release Coupling.Leder 48,(5),99-104[Diy Building Structure]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer]

Heavy Duty Steel Warehouse Storage Pallet Rack, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Professional Manufacture Hydraulic Cylinder Tube.Chenille Microfiber Mop Head. USP/SP[Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-HDPE Auto-Deflashing Plastic Bottle Blowing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Coill Nail Making Machine/Coil Nail Welding Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Boston Round Bottle With Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[10mm Tempered Bathroom Glass Safety Glass for Building]

Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Industrial Grade Anhydrous Sodium Sulfate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[American NEMA Straight Blade Plug 30A 125V/250V Power Plug]

Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector]

Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension, Part I.316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Advanced Unlocking Tool for Locksmith]

Low Cost Prefab Steel Poultry Chicken House on Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Blue Color Fish Paper/6520/6521 Flexible Composites.Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass, 175-184[Electric Magnetic Lifter for Steel Scraps on Overhead Crane]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[P4.75 Indoor Single Dual Color Advertising LED Display Billboard]

Whitaker,J.R.(1963),High Quality Factory 8ml UV Spider Gel Nail Care. Analytical Chemistry35(12), 1950-1953[China Cheap Newest 70*77cm XPE Foam 3D Wallpaper]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

500kg Capacity 14m Height Semi Electric Scissor LiftAuthor for correspondence