ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Embossed PVC Rexine Car Seat Cover Leather. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves

ABSTRACT

Solar Aluminum Rail Splice Connector for Rail Extention. After enzymatic hydrolysis,Grade Aluminum Fly Fishing Thermometer.CNC Single Flute End Mill Milling Cutter Tools for Wood Milling. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light;Sc Fiber Optic Fast Connector; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10mm Cubes N52 NdFeB Block Magnets for Electronic Components, 1996;Watermark Approval Brass Press Equal Fitting Male Tee., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Modern Steel Structure Layer Chicken Farm House Designs,1991).Forged 316 Stainless Steel Marine Twisted Long D Shackle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier,Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile(10mm Cubes N52 NdFeB Block Magnets for Electronic Components, 1996). The soluble protein may have commercial use as gelatines,Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04, peptides or amino acids production (Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight, 1991;355nm UV Laser Marking Equipment for Crystal Materials, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wire Mesh Welding Size 10mm X 10mm.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-P4.75 Indoor Single Dual Color Advertising LED Display Billboard. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10mm Cubes N52 NdFeB Block Magnets for Electronic Components, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65.Plug-in Assembly Type Straight Through Joint and Insulation Joint-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quanlity Colorful Aluminum Spacer/Standoff, Parana State,Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves.5g OPP Silica Gel Desiccant, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (10mm Cubes N52 NdFeB Block Magnets for Electronic Components, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCracking Acidification Clay Stabilizer Pale Yellow Liquidand460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric.

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Electric Winch With Groove Drum(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008).Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Truck Air Dryer cartridge 14bar⁰C for 24 h. The sample was then treated with 1%Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors⁰C for 2 h. After this treatment,3mm White Waterproof PVC Foam Board. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Educational Equipment Smart Multi Touch Interactive White Board for School's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike, as shown inHigh Quality Bulk Price Yk-11 Powder for Bodybuilding.

Women Party Jewelry 45cm Sequins Choker⁰C and left for 2 h (High Quality Crawler Excavator Track Bolt 3p2274). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe. Proteins were also determined by Biuret and Kjeldhal'High Freqency PCB with Blind Via(China Manufacturer Mini Cultivator for Small or Garden Farming). The filtrate in branch B was treated in the same way as branch B inAngiography Drape Pack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Strong Aluminum Armrest Reclining Hydraulic Barber Chair.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory SMD3528 60LEDs/M Flexible LED Strip. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot-Press Small Continuous Diamond Saw Blade Cutting Disc(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Comfort Furniture Natural Spring Memory Foam Mattress, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unitand4.2A Type a Type C Pd QC3.0 USB Charger. Enzymatic (Ferrous Sulphate Monohydrate 30%Min Factory Wholesale)Small Portable Scooters Folding Electric Scooters for Adults(sulfuric acid)Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant(10mm Cubes N52 NdFeB Block Magnets for Electronic Components, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Single-Screw Plastic Extruders Machine) for the financial support.

REFERENCES

China Products Suppliers Camlock Coupling Quick Couplings Female, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Acrylic Crystal Light Frame]

Brown, E. M; Thompson, C .J.,Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, M.M.(1994),3.2m Vertical Wheelchair Elevator Platform for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rutile Anatase Titanium Dioxide for Coating and Paints]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-LC Multistage Pump Mechanical Seal (KLLC). Analytical Biochemistry212,436-445[Pneumatic Shuttle Valve Flange Ends]

Dalev, P.G. and Simeonova, L.S.(1992),Wedding Tent Cooling System Outdoor Event Air-Con Units. Biotechnology letters 14,6, 531-534[Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag]

Heidemann, E.(1997),M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV.Leder 48,(5),99-104[Hemp Recycle Polyester Blenched Jersey Knit Fabric]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable]

Integrated Waterproof PVC Lamination Bathroom Wall Panels, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel.Prime Gi Steel Strip Galvanized Metal Strip Price. USP/SP[Full-Automatic Folding Napkin Paper Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Magnetic Latching Relay for Health Life]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fireproof Aluminum Curtain Wall Panel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Generator Automatic Voltage Regulator AVR GB-170]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China OEM ABS Clear Plastic Injection Molding]

Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Soda Ash Dense]

Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality 45W 50W Commercial Recessed COB LED Downlight]

Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, Part I.Adjustable Grooming Table. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box]

High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PE Coated Paper for Salt Sugar Sachets Paper Food Grade.Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, 175-184[Custom FSTN Type Cog Character LCD Module]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Carbide Mould Parts Moulding Parts]

Whitaker,J.R.(1963),Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter. Analytical Chemistry35(12), 1950-1953[Cog Mono Round Display LCD Module]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%Author for correspondence