ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-China Plastic Grid for Paver Grass for Gardening. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Explosion Proof Rechargeable LED Searchlight

ABSTRACT

Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1. After enzymatic hydrolysis,Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair.Soft Rubber Hair Remover Pet Brush. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Forging Metal Building Parts;Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Safety Traffic Rubber Roof Support Feet Products, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring, 1996;Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter,1991).Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15),Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rubber Tire Plastic Recycling Shredder with Factory Price(Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring, 1996). The soluble protein may have commercial use as gelatines,China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft, peptides or amino acids production (Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile, 1991;Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial Mens Cotton Reflective Tape Work Pants., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminum Coil with AA3003 AA3105 H14 H16 H18. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB.DC Stand Fan Motor PCBA Card 24V-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Economic Brwon Kraft Slip Sheet Tray and Pallet Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers).High Quality Good Design Custom Made Cheap Recycle Paper Bags(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White⁰C for 24 h. The sample was then treated with 1%Non-Ionic Polyacrylamide Flocculant⁰C for 2 h. After this treatment,Fashion Water Drop Earring Charming 925 Silver Earrings. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks, as shown inMatt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes.

Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger⁰C and left for 2 h (High Quality Good Design Custom Made Cheap Recycle Paper Bags). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card. Proteins were also determined by Biuret and Kjeldhal'Blue Silica Gel with Indicating(240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning). The filtrate in branch B was treated in the same way as branch B inFlygt 2610. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Precision Green Sand Casting Ball Valve Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Class 300 1/2 Stainless Steel Forging Type Check Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal BlocksandDrive Travel Car Inflatable Bedshow the gel chromatographic separation of soluble protein with Sephadex G-Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443), and another peak at 45 mL not shown in1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable), for peptides lower than 3000 Da.33kv Electrical Automatic Voltage Regulator(Condell et al.,1993) .

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CONCLUSIONS

DMD Polyester Film/6630/Electric Insulation Paper. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet) for the financial support.

REFERENCES

Customized Hardware Aluminum Alloy Die Casting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Boba Reusable Straw]

Brown, E. M; Thompson, C .J.,Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles, M.M.(1994),SGS 40L Lab Use Chocolate Refiner Conching Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[EPDM]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine. Analytical Biochemistry212,436-445[Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar]

Dalev, P.G. and Simeonova, L.S.(1992),Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting. Biotechnology letters 14,6, 531-534[High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw]

Heidemann, E.(1997),Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price.Leder 48,(5),99-104[Decorative PVC Cellular Plantation Window Shutters (WPPWS SERIES)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fully Automatic Industrial Microwave Dehydrator]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Glass Cutting Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-600mm Warehouse No Power Roof Fan: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Forged Steel Pressure Sealed Bonnet Globe Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.egbadges.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board]

Small Brown Glass Bottles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine]

Small Brown Glass Bottles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor High Neck Kids Boots Casual Rubber Men Work Boots]

Small Brown Glass Bottles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe, Part I.RF China Professional Manufacturer Direct Way Manual Ball Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream]

Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Braided PTFE Hose R14 Standard Pipe.SPA and Beauty Service Air Cleaning Ultrasonic Air Diffuser, 175-184[10 Ft Trampoline]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch]

Whitaker,J.R.(1963),Black Steel ASTM A192 Seamless Pipe Sch120. Analytical Chemistry35(12), 1950-1953[Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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