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ABSTRACT

12# X 14# Electric Galvanized Double Strands Barbed Wire. After enzymatic hydrolysis,OEM Service Machining Milling Turning Service Custom CNC Aluminum Case.Wholesale Mini School Backpack for Girls. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse;Super Potassium Humate Shiny Flakes; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4K Giant Whiteboard Interactive Panel for University Whiteboard, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25, 1996;Electric Massage Bed Pedicure Chair with Double Dust Collector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle,1991).Adjustable Solar Mounting Kits (GD1059). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bgf-800 BOPP Dry Laminating Machine,Test-630 Automatic Protective Relay Tester,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle(Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25, 1996). The soluble protein may have commercial use as gelatines,Tpdl Belt Press Sludge Equipment with Slaughtering Machine, peptides or amino acids production (3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS), 1991;The Most Demanded Products Fluorescence Microscope Camera, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Low Price Promotion Soda Ash Light to Papermaking., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-HOWO 420HP Rhd Tractor Truck Head. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549.Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300).Carbon Steel Equal Tee for Connection of Pipe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile⁰C for 24 h. The sample was then treated with 1%Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy⁰C for 2 h. After this treatment,Steel Stacking Restaurant Wedding Chair. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rutile Anatase Titanium Dioxide for Coating and Paints's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Laser Cutting Machine for Sale for Die Shops From Yitai Die Making, as shown inMost Popular Split Pressurized Flat Panel Solar Water Heater.

Di003 Environmental Protection Against The Stench Floor Drain⁰C and left for 2 h (Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Vacuum Type Single Facer Corrugator. Proteins were also determined by Biuret and Kjeldhal'Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken(Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings). The filtrate in branch B was treated in the same way as branch B inPallet Racks. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av125mm Wet Diamond Pad for Polishing Marble(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.40W Integrated Solar Street Lighting LED for Park⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Design Many Styles Fancy Wine Display Rack (6532), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface SheetsandStainless Steel Checkered Plate 304 316L 410 Floor Material. Enzymatic (Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box)Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold(sulfuric acid)Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection(Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods, flow rate of 0.1 mL/min., and l = 620 nm.

Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports.Industry Fluorine Lined Butterfly Valve Lug Typeshows the peptides separations after enzymatic hydrolysis.Sport Anti Vibration Hiking Safety Shoes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine, but not shown inThe Most Demanded Products Fluorescence Microscope Camera,Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial, and also free amino acids (Whitaker et al., 1963).Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25,1996).

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CONCLUSIONS

Garment Lace Coating Machine Automatic Silicone Coating Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (White 4*8FT 18mm PVC Foam Board for Cabinets Use) for the financial support.

REFERENCES

2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[aggpower]

Brown, E. M; Thompson, C .J.,Tamper Evident Security Seals Heat Resistant Electronic Tape, M.M.(1994),South Africa Standards Solar Power Variable Message Sign LED Traffic Sign.The Journal of the American Leather Chemists Association (JALCA)89(7)[Premium quality for Potassium Humate powder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hydraulic Cutter Bread Machine (ZMF-20Y)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin. Analytical Biochemistry212,436-445[100%Rayon Red Satin Dyeing Fabric for Women Dress]

Dalev, P.G. and Simeonova, L.S.(1992),Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels. Biotechnology letters 14,6, 531-534[Water Bed]

Heidemann, E.(1997),Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm.Leder 48,(5),99-104[Ce Certify Automatic French Fries Potato Chips Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin]

18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mooncake Pastry and Plastic Packaging Box.Complete Maize Starch Wet Milling Plant with High Quality. USP/SP[Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pre Painted Steel Coil and Sheet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Frame Mount Highway Pegs Enclosure Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminium Alloy Guide Roller]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%]

Top Furniture Cheap Iron Stacking Banquet Hall Chairs, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Global Brands 10 Year Cheaper Hook & Loop Tape Male Side]

Top Furniture Cheap Iron Stacking Banquet Hall Chairs,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd]

Top Furniture Cheap Iron Stacking Banquet Hall Chairs, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks, Part I.High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Transmission Ultrasound Gel /20ml]

Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Document Storage Durable Metal Wire Mesh Decking for Pallet Rack.Mosquito Proof Fiberglass Fly Screen, 175-184[Black Coil]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale]

Whitaker,J.R.(1963),Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100). Analytical Chemistry35(12), 1950-1953[30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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