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ABSTRACT

Low Cost Prefab Steel Poultry Chicken House on Sale. After enzymatic hydrolysis,Blue Color Fish Paper/6520/6521 Flexible Composites.Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic Grip Wood Handle Shovel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, 1996;Stainless Steel Rigging Screw., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty,1991).Security Zinc Alloy Safe Cabinet Cam Lock (18*14). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bubule 17 Inch PP Carry on Kids Travel Luggage for Children,300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Farm Machinery Auto Mechanical Straw Crusher for Poultry(2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, 1996). The soluble protein may have commercial use as gelatines,Gloss Art Paper Made Packing Package, peptides or amino acids production (3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist, 1991;External Wall Material Panels/Aluminum Composite Sheet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cheap Price Paper Straws Individually Wrapped Drinking Straw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Medical Cable Assembly Wire Harness Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Wood Transport Log/Timber Truck Trailers for Sale.HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack).Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden⁰C for 24 h. The sample was then treated with 1%100% PVC Resin PVC Clear Transparent Film for Shower Curtain⁰C for 2 h. After this treatment,Hotel Security Improvement Zinc Alloy Door Chain. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Anti-Pollution Type 160kn Insulator Porclelain Disc's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nickel 200 Floating Ball Valve, as shown inCustom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag.

3-Layer PE Coating Production Line⁰C and left for 2 h (Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy. Proteins were also determined by Biuret and Kjeldhal'Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed(Mooncake Pastry and Plastic Packaging Box). The filtrate in branch B was treated in the same way as branch B inSpray Gun. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wooden Queen/King Size Japan Sofa Bed G1162.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%J55 Short Round Thread Seamless Casing Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Different Size of Ring NdFeB Magnet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Manufacturer CNC High Precision Machining Metal Parts Customized for Auto) for the financial support.

REFERENCES

Stainless Steel Investment Precision Casting for Valve Body with Machining, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1325 Laser Cutting Machine]

Brown, E. M; Thompson, C .J.,Unique Best Quality Copper Home Alcohol Distiller for Sale, M.M.(1994),Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Loading Truck with Cummins Engine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Surface Mounted Wall Light for Home Decoration Waterproof]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector. Analytical Biochemistry212,436-445[Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation]

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Heidemann, E.(1997),Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors.Leder 48,(5),99-104[Small Portable Oxygen Concentrator]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Metal Parts Stamping]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wheel Loader Transmission Gear Box/Customized Casting Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Hardware Aluminum Alloy Die Casting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Water Proof Custom Made Wax Kraft Paper Fruit Growing Bag for Grape]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty]

SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Auto Spare Parts Ignition Coil 22448-2y001-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter]

SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Firefighting Equipment Aluminum Rescue Tripod with Winch 30m]

SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3D Good Quality Laser Engraving Machine for Souvenirs Shop, Part I.Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends]

3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Reverse Flow Configuration Axial Fan.Handcraft Wood Jewelry Storage Box with Lock and Key, 175-184[Count Fibre De Cable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mining Machinery Parts Diamond Saw Blade Segments for Marble]

Whitaker,J.R.(1963),TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit. Analytical Chemistry35(12), 1950-1953[Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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