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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Car Trunk Organizer Car Storage Organizer


ABSTRACT

Fashion Cotton Baby/Kid Hat (DH-BF332). After enzymatic hydrolysis,Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line.OEM Round Stainless Steel Fruit Plate Tray. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory Supply Industry Grade Sodium Gluconate;Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mini High Temperature Clothing Labels Screen Printing Machine Factory, 1996;Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale,1991).Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Conventional Best-Selling Household Bamboo Craft Chopsticks,High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ce Marked Medical Syringe Pump (WP08N)(Mini High Temperature Clothing Labels Screen Printing Machine Factory, 1996). The soluble protein may have commercial use as gelatines,New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty, peptides or amino acids production (Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet, 1991;CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electro Galvanized Low Carbon Straight Cut Wire., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Geotextile for Roofs, Civil Applications. Railway Work.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pneumatic Shuttle Valve Flange Ends. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mini High Temperature Clothing Labels Screen Printing Machine Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Stx825 Dual 15 Powerful PA Speaker, Speaker Box.Ce Approval Biscuit Trayless Packing Machine (MZ-250B)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm, Parana State,Car Trunk Organizer Car Storage Organizer.White Pattern Acrylic Sheet for Decoration Produced by Senpu, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Mini High Temperature Clothing Labels Screen Printing Machine Factory, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWalnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Boardand5 Liter Packed Medical Ultrasound Gel with Ce and ISO.

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Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet).SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production0C for 24 h. The sample was then treated with 1%110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator0C for 2 h. After this treatment,Supply 20-110mm PP Reducing Tee for Pipe Systems. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)5 Gallon Pure Water Bottle Non-Spill Cap's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks, as shown inLivining Room Rugs Geometric Patterns Carpets 6′*9′.

7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care0C and left for 2 h (4.2A Type a Type C Pd QC3.0 USB Charger). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Solid Carbide Single Flute End Mills Oblique Angle Cutting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies. Proteins were also determined by Biuret and Kjeldhal'ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat(Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls). The filtrate in branch B was treated in the same way as branch B in90 Grams Menta Original Fresh Breath Whitening Toothpaste. The final soluble protein shown were used for peptides separations using gel permeation chromatography,400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Small Capacity Automatic Cup Filling Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavSeasoned Cast Iron South Africa Three Legged Potjie Pot(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 New Release Doypack Sachet Powder Packing Machine Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Amplifier Tube Glass Vacuum Tube (833C), pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High SchoolandGalvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools. Enzymatic (New Style Custom Logo Makeup Multi Colored Eyeshadow Palette)Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending(sulfuric acid)20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale(Mini High Temperature Clothing Labels Screen Printing Machine Factory, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu, flow rate of 0.1 mL/min., and l = 620 nm.

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Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500andSupplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bendingshow the gel chromatographic separation of soluble protein with Sephadex G-700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit.Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files, and another peak at 45 mL not shown in5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler, for peptides lower than 3000 Da.New Industrial Wheeled Wooden Shoe Display Fixtures for Shop(Condell et al.,1993) .

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CONCLUSIONS

Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler) for the financial support.

REFERENCES

China Spplier 70W Solar Street Light with Camera Monitoring System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Disposable Femoral Transradial Medical Device Radial Catheter Introducer Sheath]

Brown, E. M; Thompson, C .J.,Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud, M.M.(1994),Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Resin Trap Strainer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape. Analytical Biochemistry212,436-445[ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat]

Dalev, P.G. and Simeonova, L.S.(1992),Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader. Biotechnology letters 14,6, 531-534[Drug Delivery ABS Hospital Medical Treatment Medicine Cart]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment]

Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory.Masturbators Adult Sex Toys Japanese Love Doll. USP/SP[Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Density Artificial Grass Football Playground Surfaces (STO)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Manufacturer High Strength Carbon Steel Heavy Hex Nut]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3D Self-Adhesive Faux Foam Bricks Wallpaper for TV Walls]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Anti-Pollution Type 160kn Insulator Porclelain Disc]

Carbon Steel Spherical Plain Radical Bearings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb]

Carbon Steel Spherical Plain Radical Bearings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone]

Carbon Steel Spherical Plain Radical Bearings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike, Part I.IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[10in 1500lb Pressure Seal Forged Steel Swing Check Valve]

Whitaker,J.R.(1963),Petrochemical Loading Platform Folding Stairs. Analytical Chemistry35(12), 1950-1953[Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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