ARTICLES

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ABSTRACT

1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll. After enzymatic hydrolysis,UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.Oil Wax Furniture PVC Leather (HONGJIU-498#). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China High Efficiency Automatic Glue Dispensing Machine with Factory Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Machining Mechanical Parts Fabrication Service, 1996;Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall,1991).Electronic Pulse Gas Stove Burner Igniter Transformer Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Turned Aluminum Anodized Auto Part,Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Commercial Vegetable Mushroom Coconut Meat Drying Machine(CNC Machining Mechanical Parts Fabrication Service, 1996). The soluble protein may have commercial use as gelatines,4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass, peptides or amino acids production (Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, 1991;Hydraulic Fold-Bend Machine Hydraulic Bending Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Delphi Control Valve 28239295 (622B)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Precision Aluminum Alloy/Zinc Alloy Die Casting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Machining Mechanical Parts Fabrication Service, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bathroom Makeup LED Mirror Glass with Light.Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

PP Polypropylene Two Layer Film Blowing Machine, Parana State,Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials.Disposable Sugarcane Pulp Paper Bowl 500ml, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CNC Machining Mechanical Parts Fabrication Service, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Bedding Set Hotel 100% Cotton Bed Sheets WhiteandDsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector.

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Flat Roof Insulation Board Heat Insulation Board(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production).2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Female Threaded Tee T Shape Pipe Fittings⁰C for 24 h. The sample was then treated with 1%Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection⁰C for 2 h. After this treatment,Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste, as shown inBaby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042).

Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools⁰C and left for 2 h (Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ballscrew Fixed Support Unit Fk12 with High Quality for The Module. Proteins were also determined by Biuret and Kjeldhal'Natural OEM Private Label Customized Skin Care Face Serum(China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps). The filtrate in branch B was treated in the same way as branch B inDiamond Tile Cutting Disc. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Screw Press Dewatering Machine for Cassava Residue. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor Indoor Football Soccer Goal Post Net for Kids(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale High Speed Mixer Blender for Extruder Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Single Block Vertical Wire Drawing Machine for Screw and Bolt Making.UV Resistant Durable HDPE Reinforced Geomembrane HDPEshows the peptides separations after enzymatic hydrolysis.China Professional Shipping Agent to Turkey(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlat Prefab Shipping Container Home Prefabricated Modular Pack Movable House, but not shown in10mm AISI304 Carbon Steel Balls Motor Bearing for Slide,White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment, and also free amino acids (Whitaker et al., 1963).Crusher for Animal Waste/ Chicken Manure. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Machining Mechanical Parts Fabrication Service,1996).

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Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card, V_e, and K_avcalculated as discussed previously.Disposable Sterile Safety Blood Lancet,Plush Comfort Spring Mattress Cheap Price for Wholesale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent) (Reis et al.,1989;Headband Big Headphone EVA Bag Tool Case Specialized Storage Box.,1991;Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch.,1991).3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss(1992), were lower, except for cystine and tyrosine,Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway.

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CONCLUSIONS

Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Drinking Mineral Water Filling Line/Water Bottling Line) for the financial support.

REFERENCES

1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car Led Advertising Display]

Brown, E. M; Thompson, C .J.,Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain, M.M.(1994),Waterproof LED Flash Shoe Light Crystal Epoxy Resin.The Journal of the American Leather Chemists Association (JALCA)89(7)[Nail Stamping Plates Custom Metal Part Sheet Metal Stamping]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Shopping Paper Bag for Clothing Package. Analytical Biochemistry212,436-445[Seasoned Cast Iron South Africa Three Legged Potjie Pot]

Dalev, P.G. and Simeonova, L.S.(1992),Qingdao Us Type Load Binder Lever Chain Tensioner. Biotechnology letters 14,6, 531-534[Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway]

Heidemann, E.(1997),Stainless Steel Elbow SS304 SS316L.Leder 48,(5),99-104[Aluminum Plate 7075 T6]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator]

Water Bed, Water Slide, Water Roller for Indoor Playground, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD.Outdoor 15kv Pole Mounted Automatic Circuit Recloser. USP/SP[New Design Many Styles Fancy Wine Display Rack (6532)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[European Hotel Decorative Natual Shell Square Standing Floor Lamp]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Indoor Kiosk]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4 Gang 1 Way Electrical Fitting Ce Certificate]

14 Heads Double Door Multihead Weigher, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale High Speed Mixer Blender for Extruder Machine]

14 Heads Double Door Multihead Weigher,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old]

14 Heads Double Door Multihead Weigher, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:White Marble, Carrara White, Bianco Carrara Marble for Sale, Part I.Upper Limbs Machine/Spare Parts for Fitness Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material]

Cvkr Silent Check Valve Dn350, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Best Shatterproof Gold Rim Wine Glasses.10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting, 175-184[Gaf]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2017 New Design Vinyl 3D Stone Wallpaper Factory in China]

Whitaker,J.R.(1963),High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea. Analytical Chemistry35(12), 1950-1953[New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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