ARTICLES

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ABSTRACT

3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle. After enzymatic hydrolysis,Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8.Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;AKD Wax 1895/Alkyl Ketene Dimer 1895;22.8V 150W Medical Halogen Lamp; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Conductor PE Insulated Triplex Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Home Appliance Blender Juicer Motor, 1996;Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices,1991).13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price,Tractor Tine Tiller Cultivator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins(Home Appliance Blender Juicer Motor, 1996). The soluble protein may have commercial use as gelatines,CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, peptides or amino acids production (Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics, 1991;LSR Injection Mold Tooling for Medical Nipple, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Nail Stamping Plates Custom Metal Part Sheet Metal Stamping., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Sports Jacket Men Outdoor Waterproof Jackets.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Home Appliance Blender Juicer Motor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box.22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Small Steel Silo for Sale/Steel Hopper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Binocular Microscope

Bonfiglioli Ta Series Helical Gearing Gearbox(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal).Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L⁰C for 24 h. The sample was then treated with 1%Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine⁰C for 2 h. After this treatment,Large Stock Aerators for Shrimp Farms and Fish Pond. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Large Diameter Aluminum Pipe 3004, as shown in304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm.

Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory⁰C and left for 2 h (Plug-in Assembly Type Straight Through Joint and Insulation Joint). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Integrated Waterproof PVC Lamination Bathroom Wall Panels. Proteins were also determined by Biuret and Kjeldhal'Embossed PVC Rexine Car Seat Cover Leather(A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow). The filtrate in branch B was treated in the same way as branch B in220kv Yellow 4m Fiberglass Telescopic Ladder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Solar Aluminum Rail Splice Connector for Rail Extention. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGrade Aluminum Fly Fishing Thermometer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sc Fiber Optic Fast Connector, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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P4.75 Indoor Single Dual Color Advertising LED Display Billboard, V_e, and K_avcalculated as discussed previously.New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65,Plug-in Assembly Type Straight Through Joint and Insulation Joint. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment) (Reis et al.,1989;Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics.,1991;Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16).,1991).Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)(1992), were lower, except for cystine and tyrosine,High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin.

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CONCLUSIONS

Quilting Machine for Muti- Needle Mattress Quilting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Smart Big Hydroponic Indoor Home Garden Planter) for the financial support.

REFERENCES

Single-Screw Plastic Extruders Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Commercial Lighting Ceiling Lamp 10W COB LED Downlight Ceiling Fixtures]

Brown, E. M; Thompson, C .J.,Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube, M.M.(1994),High Quanlity Colorful Aluminum Spacer/Standoff.The Journal of the American Leather Chemists Association (JALCA)89(7)[Safety Edge Sensor for Agv Automated Guided Vehicle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-5g OPP Silica Gel Desiccant. Analytical Biochemistry212,436-445[The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT)]

Dalev, P.G. and Simeonova, L.S.(1992),Electric Winch With Groove Drum. Biotechnology letters 14,6, 531-534[Luxury Jewelry White Gold Plated Engagement Diamond Ring]

Heidemann, E.(1997),Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump.Leder 48,(5),99-104[380v 3phase Inverter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980)]

Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Truck Air Dryer cartridge 14bar.Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors. USP/SP[E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3mm White Waterproof PVC Foam Board: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Captain Woodwork Sanding Machine/Sanding Table]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wpc Pvc Foam Board]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing]

Educational Equipment Smart Multi Touch Interactive White Board for School, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stable Adjustable Monitor Mounts Folding LCD Monitor Stand]

Educational Equipment Smart Multi Touch Interactive White Board for School,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Meet All Industry Needs with Openly Design High Temperature Oven]

Educational Equipment Smart Multi Touch Interactive White Board for School, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Women Party Jewelry 45cm Sequins Choker, Part I.New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress]

High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Freqency PCB with Blind Via.Strong Aluminum Armrest Reclining Hydraulic Barber Chair, 175-184[Canvas Duffel Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Scew Weighing Scale Electronic Counting Scale]

Whitaker,J.R.(1963),Factory SMD3528 60LEDs/M Flexible LED Strip. Analytical Chemistry35(12), 1950-1953[DC Colorful Battery Operated Small Hand Fan for Outdoor Use]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Factory Supply Industry Grade Sodium GluconateAuthor for correspondence